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A test method for detecting the potential toxic influence of stomach poisoning insecticide compounds on folsomia candida

A test method and compound technology, applied in the direction of animal husbandry, etc., can solve the problems such as the direct toxicity effect of Bai Fu jumping, which is not relatively perfect, and achieve the effect of a simple method.

Inactive Publication Date: 2015-11-25
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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Problems solved by technology

However, previous work mainly focused on the impact of transgenic insect-resistant plants on the dynamics of collembola communities through field investigations. At present, there is no relatively complete method for evaluating the direct toxicity of insecticides or transgenic insecticidal proteins on Baifutiao. test system

Method used

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  • A test method for detecting the potential toxic influence of stomach poisoning insecticide compounds on folsomia candida
  • A test method for detecting the potential toxic influence of stomach poisoning insecticide compounds on folsomia candida

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Embodiment Construction

[0017] 1 Construction of toxicity assay system

[0018] The following describes the specific implementation of the test method for detecting the potential toxicity of the stomach poisonous insecticidal compounds (including transgenic insecticidal proteins such as Bt protein) provided by the present invention on the potential toxicity of soil springtails.

[0019] Baifutiao Feed: To establish and determine the direct toxicity of insecticidal compounds to test insects, a suitable insect feed is first required, through which the insecticidal compounds are fed to the test insects. In this toxicity test system, the feed used must be able to meet the normal growth and development of the tested insects (generally require survival rate >80%) (Romeis et al., 2011; Lietal., 2013). Through experimental research, it is found that the survival rate of Baifutiao is >85% ( figure 1 ), meeting the basic requirements for establishing a toxicity assay system.

[0020] Baifutiao feeding method...

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Abstract

The invention provides a test method for detecting the potential toxic influence of stomach poisoning insecticide compounds on folsomia candida. The method is characterized in that an insecticide or transgenic insecticidal protein is added into yeast powder (feed) uniformly and the mixture is directly delivered to folsomia candida; yeast mixed with a protease inhibitor with a concentration of 75 micrograms E-64 / g is used for positive control treatment and feed without any insecticide compounds is used for negative control treatment; potential toxicity of the tested insecticide compounds on spring tails is assessed by comparing the important life parameters such as the survival rate, the head breadth, the body length and the egg laying amount of folsomia candida of the treatment group and the control groups. By using the method, the biological reaction of folsomia candida to potassium arsenate [PA], E-64 and galanthus nivalis agglutinin (GNA) with different concentrations can be tested. It is shown that the different life parameters of folsomia candida show an obvious dose-effect relationship along with the change of the concentrations of PA, E-64 and GNA, so that the effectiveness and the sensitivity of the method are testified.

Description

technical field [0001] The invention relates to a test method for detecting the potential toxic effects of gastric poisonous insecticidal compounds (including transgenic insecticidal proteins such as Bt protein) on the potential toxicity of soil collembola. Background technique [0002] Springtails, also known as springtails, are a class of small soil arthropods that are widely distributed. They are important decomposers in the soil ecosystem and one of the driving forces of the material cycle in nature. They play a very important role in regulating the cycle of soil nutrients. role. Because collembola is very sensitive to soil quality and toxic chemicals in soil, the diversity, community structure and species composition of collembola can play a good indicator role in soil quality and pollution status. [0003] Folsomiacandida is the most common type of collembola, the most sensitive to chemical substances, parthenogenetic and easy to raise artificially. Therefore, it is w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
Inventor 李云河杨艳林克剑陈秀萍彭于发
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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