Enzyme process extracted Radix angelicae anomalae polysaccharide, and preparation method and application thereof

An enzymatic extraction and Angelica polysaccharide technology, applied in the field of biomedicine, can solve the problems of affecting enzyme activity, not yet carried out research on the structure and activity of Angelica polysaccharide, and high cost, and achieve the effects of reducing reaction steps, good purification effect and low production cost.

Active Publication Date: 2017-08-04
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Enzyme belongs to protein, its type, enzymatic reaction temperature, pH, time and substrate amount will all affect the activity of the enzyme, too high or too low will cause the inactivation of the enzyme, thus affecting the extraction efficiency
At the same time, because of the large amount of enzyme preparation and high cost of enzymatic hydrolysis, its large-scale application in the polysaccharide extraction industry is limited.
[0007] At present, the research on polysaccharides of Angelica dahurica is not deep enough
Only a few scholars have analyzed the extraction of polysaccharides of Angelica dahurica, anti-oxidation and enhancing the immune function of animal skin tissue, and no research has been carried out on the structure and activity of polysaccharides extracted by enzymatic methods

Method used

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  • Enzyme process extracted Radix angelicae anomalae polysaccharide, and preparation method and application thereof
  • Enzyme process extracted Radix angelicae anomalae polysaccharide, and preparation method and application thereof
  • Enzyme process extracted Radix angelicae anomalae polysaccharide, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Preparation of Polysaccharides from Radix Angelica dahurica

[0044] The preparation method of Radix Angelica dahurica polysaccharide comprises the following steps:

[0045] A: degreasing and decolorization

[0046] Take the root of Baizhi root, wash it, dry it at 45°C, and crush it through a 40-mesh sieve. Weigh the powder sample of Angelica dahurica, add petroleum ether to reflux and degrease for 4 hours, dry at 45°C, then add 80% ethanol and extract at 80°C for 4 hours to remove part of the pigment and oligosaccharides, evaporate the ethanol and crush it, dry at 45°C for later use . The consumption of sherwood oil is calculated as 3mL by every gram of Chuan Baizhi powder, and the consumption of 80% ethanol is calculated as 2mL per gram of Chuan Baizhi powder.

[0047] B: extract

[0048] Weigh 500g of the powder after degreasing and depigmentation, add 4 times the amount of citric acid-sodium citrate buffer solution containing 0.45% cellulase and pH value of 4.5,...

Embodiment 2

[0062] Preparation of Polysaccharides from Radix Angelica dahurica

[0063] The preparation method of Radix Angelica dahurica polysaccharide comprises the following steps:

[0064] Step A: Compared with Step A of Example 1, the drying temperature is 50° C., the amount of petroleum ether is 2 mL per gram of Baizhi powder, and the rest of the conditions are the same.

[0065] Step B: Weigh 500g of the powder after degreasing and depigmentation, add 2 times the amount of citric acid-sodium citrate buffer solution containing 0.45% cellulase and pH value of 4.5, extract in a water bath at 40°C for 240 minutes, and inactivate the enzyme in a boiling water bath for 15 minutes live, centrifuged at 4000r / min for 5min to collect the supernatant, repeated the extraction 4 times, combined the extracts and concentrated to obtain a concentrate.

[0066] Step C to step G are the same as in Example 1, to obtain.

Embodiment 3

[0068] Preparation of Polysaccharides from Radix Angelica dahurica

[0069] The preparation method of Radix Angelica dahurica polysaccharide comprises the following steps:

[0070] Step A: Compared with Step A of Example 1, the drying temperature is 40°C, the amount of petroleum ether is 4mL per gram of Baizhi powder, and the rest of the conditions are the same.

[0071] Step B: Weigh 500g of the powder after degreasing and depigmentation, add 6 times the amount of citric acid-sodium citrate buffer solution containing 0.45% cellulase and pH value of 4.5, extract in a water bath at 60°C for 120 minutes, and in a boiling water bath for 5 minutes to inactivate the enzyme live, centrifuged at 4000r / min for 5min to collect the supernatant, extracted once, combined the extracts and concentrated to obtain a concentrate.

[0072] Step C to step G are the same as in Example 1, to obtain.

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Abstract

The invention discloses an enzyme process extracted Radix angelicae anomalae polysaccharide, and a preparation method and an application thereof. The Radix angelicae anomalae polysaccharide has alpha-configuration and beta-configuration polysaccharide linking modes, the apparent molecular weight of the Radix angelicae anomalae polysaccharide is 3.93 kDa. The Radix angelicae anomalae polysaccharide is obtained through enzyme process extraction of a Radix angelicae anomalae medicinal material, alcohol precipitation and impurity removal, protein and salt removal, and DEAE-agarose gel FF column and agarose gel 6FF gel column chromatography separation. The Radix angelicae anomalae polysaccharide obtained in the invention has good whitening activity and has an inhibitory effect on the tyrosinase activity, and the tyrosinase inhibition rate gradually increases with the increase of concentration, and the dose-effect relationship is obvious. The method for extracting the Radix angelicae anomalae polysaccharide has the advantages of small dosage of an enzyme preparation, simple process and low production cost.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to an enzymatically extracted polysaccharide of Radix Angelica dahurica, a preparation method and application thereof. Background technique [0002] Angelica dahurica (Fisch.ex Hoffm) Benth.etHook.f. or Hangbaizhi Angelica dahurica (Fisch.ex Hoffm) Benth.et Hook.f.var.formosana (Boiss) Shan et Yuan Dried roots. Angelica dahurica is pungent in taste, warm in nature, and returns to the stomach, large intestine, and lung meridians. It has the effects of relieving exterior cold, expelling wind and relieving pain, dredging nasal orifices, drying dampness and stopping belt, reducing swelling and discharging pus, and is used for cold headache, brow bone pain, nasal congestion, runny nose, nasal congestion, nasal sinusitis, toothache, belt Next, the sores are swollen and painful. [0003] Angelica dahurica is one of the 87 kinds of Chinese herbal medicines announced by th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12P19/14C08B37/00
CPCC08B37/0003C08B37/006C12P19/04C12P19/14
Inventor 吴卫唐小琴侯凯何婧芝何梦竹汪丽霞温小冬王齐飞
Owner SICHUAN AGRI UNIV
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