Preparation method and application of absorbable collagen sponge

A technology of collagen sponge and reaction, which can be used in absorbent pads, pharmaceutical formulations, medical science, etc., and can solve the problems of biocompatibility, poor mechanical properties, and molecular weight reduction.

Inactive Publication Date: 2021-02-12
HANGZHOU SINGCLEAN MEDICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Gelatin sponge is no longer biologically active due to the destruction of the three-dimensional helical structure of collagen, and its molecular weight is reduced, resulting in poor mechanical properties and is extremely easy to degrade; while the cross-linked collagen sponge has significantly improved mechanical properties, but is biocompatible. Properties are compromised by the presence of potentially toxic crosslinkers

Method used

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  • Preparation method and application of absorbable collagen sponge
  • Preparation method and application of absorbable collagen sponge
  • Preparation method and application of absorbable collagen sponge

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Bovine Achilles Tendon Preconditioning:

[0031] Rinse and thaw the bovine Achilles tendon, peel off the fascia, soak it in 0.9% sodium chloride solution, take it out and wash it, and soak it in 75% ethanol. Rinse with purified water, freeze and slice.

[0032] Collagen Extraction and Purification:

[0033] Weigh 400g of beef Achilles tendon slices, thaw with 0-10°C pre-cooled water for injection and soak for about 15 minutes;

[0034] Use 3000ml 2g·L -1 Calcium chloride solution was used to wash the bovine Achilles tendon slices twice, each time for 20 minutes.

[0035] Soak the beef Achilles tendon slices in 1000ml 75% ethanol for 20 minutes. Then wash three times with pre-cooled injection.

[0036] The beef Achilles tendon slices were placed in a tissue masher several times to mash them, and placed in 100-mesh silk, squeezed and shredded.

[0037] Add 8000ml of 4% acetic acid to the shredded Achilles tendon to make it swell, and then place it in a constant temp...

Embodiment 2

[0045] Bovine Achilles Tendon Preconditioning:

[0046]Rinse and thaw the bovine Achilles tendon, peel off the fascia, soak it in 0.9% sodium chloride solution, take it out and wash it, and soak it in 75% ethanol. Rinse with purified water, freeze and slice.

[0047] Collagen Extraction and Purification:

[0048] Take out the beef Achilles tendon slices, thaw them with 8°C pre-cooled water for injection and soak them for about 30 minutes;

[0049] Use 2000ml 1.5g·L -1 Calcium chloride solution was used to wash the bovine Achilles tendon slices twice, each time for 30 minutes.

[0050] Soak the beef Achilles tendon slices in 700ml 75% ethanol for 30 minutes. Then wash three times with pre-cooled injection.

[0051] The beef Achilles tendon slices were placed in a tissue masher several times to mash them, and placed in 50-mesh silk, squeezed and shredded.

[0052] Add 4000ml of 2% citric acid to the shredded Achilles tendon to make it swell, and then place it in a constant...

Embodiment 3

[0060] Embodiment 3: the collagen sponge of embodiment 1, embodiment 2 is carried out physicochemical and biological index detection, and the result is as follows:

[0061]

[0062]

[0063] The product has a high water absorption capacity, which is beneficial to clinical hemostasis; and has excellent tensile properties. When used as a dressing, it can maintain a good shape and is not easy to deform.

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Abstract

The invention relates to a preparation method and application of absorbable collagen sponge and belongs to the technical field of biomedical materials. The collagen sponge is prepared through low-temperature extraction, freeze drying and electron beam irradiation sterilization, the triple helix structure of the collagen sponge is not damaged, and good biological activity is kept. Through biological evaluation and animal experiment research, the prepared sample has a remarkable hemostatic effect, and the degradation performance and the anti-adhesion effect of the collagen sponge remained in thebody after hemostasis can better meet the requirements. The sample has no obvious influence on blood routine examination, blood coagulation, blood biochemistry, immune organs, liver and kidney functions and the like. The product has great potential in the aspects of clinical hemostasis, wound healing, bone defect, nerve repair and the like.

Description

technical field [0001] The invention relates to a preparation method and application of an absorbable collagen sponge, belonging to the technical field of biomedical materials. Background technique [0002] Collagen is the protein with the highest content and the widest distribution in animals. It is the main structural protein of the body and the main component of supporting tissues and connective tissues. It is a fibrous protein with a unique three-dimensional helical structure ( figure 1 ). It consists of two α 1 chain and 1 alpha 2 Composed of polypeptide chains, three α-peptide chains are intertwined to form a rope-like superhelical structure, each collagen chain is a left-handed helical configuration, and the three left-handed helical chains are intertwined to form a right-handed helical structure, forming a unique triple helix of collagen structure, making its molecular structure very stable. The unique structural characteristics of collagen endow it with many us...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/32A61L15/42A61L15/44
CPCA61L15/325A61L15/425A61L15/44A61L2300/412A61L2300/418A61L2400/04
Inventor 刘超远高田芳黄伟
Owner HANGZHOU SINGCLEAN MEDICAL PROD
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