Biological detection method used for quality evaluation on and quality control over heat-clearing and detoxifying traditional Chinese medicines

A technology for clearing away heat and detoxification, and quality evaluation. It is applied in biochemical equipment and methods, microbial determination/inspection, and measurement devices. It can solve the problems of non-pharmacological components and low bioavailability, and achieve high-quality and effective results.

Active Publication Date: 2017-04-26
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, the quality standard of Grass Cortex is to control its quality through a single index of isopyridine, but isopyridine is obviously not its main active ingredient; The content determination of several isoquinoline alkaloids such as palmatine is used to control their quality, but the general oral bioavailability of Coptidis alkaloids is extremely low. Especially good at

Method used

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  • Biological detection method used for quality evaluation on and quality control over heat-clearing and detoxifying traditional Chinese medicines
  • Biological detection method used for quality evaluation on and quality control over heat-clearing and detoxifying traditional Chinese medicines
  • Biological detection method used for quality evaluation on and quality control over heat-clearing and detoxifying traditional Chinese medicines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Preparation of reference solution: take appropriate amount of quercetin, quercetin and gallic acid reference substance, accurately weigh, add methanol to make a mixed solution containing 15µg of quercetin, 15µg of quercetin and 20µg of gallic acid per 1ml. That's it.

[0033] Preparation of the test product: take an appropriate amount of the test product, mix well, take about 0.1g, accurately weigh it, put it in a 25mL volumetric flask, add 10mL of water to dissolve it (ultrasound can be used to dissolve it if necessary), and add methanol to the volume Scale, filter (0.45µm microporous membrane), take the continuous filtrate, that is, it is obtained.

[0034] 1. Chromatographic conditions

[0035] Mobile phase: A: B = acetonitrile: 0.1% phosphoric acid aqueous solution (gradient elution procedure is shown in Table 1 below), column temperature: 35 degrees, flow rate: 1.0 ml / min, wavelength: 254 nm.

[0036]

[0037] Precisely pipette the mixed reference solution (qu...

Embodiment 2

[0040] Example 2 The method of anti-rabbit erythrocyte aggregation to characterize the anti-influenza virus activity of traditional Chinese medicine for clearing heat and detoxifying

[0041] Alsever's solution: take 0.5g of citric acid monohydrate, 8.0g of trisodium citrate dihydrate, 20.5g of glucose, and 4.2g of sodium chloride, dissolve in water and dilute to 1L (about pH 6.2), and then dispense the bacteria, let it cool, and store at 4°C for later use.

[0042] Preparation of 1% erythrocyte suspension: Take 8 mL of rabbit blood, mix it with an equal volume of Ashford solution, wash 3 times with an appropriate amount of PBS, and wash with 2000 r·min for the last time. -1 Centrifuge for 10 min, take 1 mL of packed red blood cells, make 100 mL of 1% red blood cell suspension with PBS, and store at 4°C for later use.

[0043] Phosphate Buffered Saline (PBS): Take 1.10 g of anhydrous disodium hydrogen phosphate, 0.32 g of anhydrous sodium dihydrogen phosphate, and 8.50 g of ...

Embodiment 3

[0057] Example 3 The method of resisting spleen enlargement in mice to characterize the immune and anti-inflammatory activity of traditional Chinese medicines for clearing heat and detoxifying

[0058] Forty-eight SPF grade C57 female mice (13-20 g) were weighed, and their back hair was shaved with a razor to form a skin area of ​​about 2 cm × 3 cm, and were housed in a single cage. According to body weight, they were randomly divided into 6 groups, with 8 animals in each group, namely blank group (NG), model group (MG), high-dose group (BH, 0.112g / mL), and low-dose Angelica alcohol extract. group (BL, 0.056g / mL), high-dose group of monkey earring water extract (HH, 0.256g / mL) and low-dose group of monkey earring water extract (HL, 0.128g / mL).

[0059] The blank group smeared 80 mg of Vaseline on the back of the mice regularly every day, and at the same time, the mice were gavaged with normal saline (the dosage was 0.04 mL g -1 ), the model group smeared 80 mg of imiquimod cr...

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Abstract

The invention provides a biological detection method used for quality evaluation on and quality control over heat-clearing and detoxifying traditional Chinese medicines. The biological detection method comprises the steps of firstly adopting a method of anti-rabbit erythrocytea condensation outside the body to represent anti influenza virus activity of heat-clearing and detoxifying traditional Chinese medicines, then adopting a method of anti-mouse spleen swelling method inside the body to represent immune anti-inflammatory activity of heat-clearing and detoxifying traditional Chinese medicines, comparatively completely representing activity of anti-pathogenic microorganisms and anti-inflammatory-immune regulation of heat-clearing and detoxifying medicinal materials through the combination of identification experiments inside and outside the body, and identifying the quality of the medicinal materials through a biological evaluation method. The biological detection method used for the quality evaluation on and the quality control over the heat-clearing and detoxifying traditional Chinese medicines provides a more objective quantitative evaluation method for the clinical effectiveness of the medicinal materials, and guarantees high quality and effectiveness of the medicinal materials.

Description

technical field [0001] The invention relates to the technical field of quality control of traditional Chinese medicines, and more particularly, to a biological detection method for quality evaluation and quality control of traditional Chinese medicines for clearing heat and detoxifying. Background technique [0002] The development of Chinese herbal medicine identification methods has been synchronized with the development of Chinese medicine, which is reflected in the medical books and herbal records of past dynasties. After the introduction of western pharmacognostics identification methods into China, four major identification method systems of basic, morphological, microscopic and physicochemical identification of traditional Chinese medicine have been formed. Then my country innovatively applied powder identification in the microscopic identification method. At present, the thin-layer method has become the mainstream of physical and chemical identification methods of t...

Claims

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Application Information

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IPC IPC(8): C12Q1/02
CPCG01N33/5008G01N33/5044
Inventor 潘卫松潘超美
Owner GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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