Biological detection method used for quality evaluation on and quality control over heat-clearing and detoxifying traditional Chinese medicines
A technology for clearing away heat and detoxification, and quality evaluation. It is applied in biochemical equipment and methods, microbial determination/inspection, and measurement devices. It can solve the problems of non-pharmacological components and low bioavailability, and achieve high-quality and effective results.
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Embodiment 1
[0032] Preparation of reference solution: take appropriate amount of quercetin, quercetin and gallic acid reference substance, accurately weigh, add methanol to make a mixed solution containing 15µg of quercetin, 15µg of quercetin and 20µg of gallic acid per 1ml. That's it.
[0033] Preparation of the test product: take an appropriate amount of the test product, mix well, take about 0.1g, accurately weigh it, put it in a 25mL volumetric flask, add 10mL of water to dissolve it (ultrasound can be used to dissolve it if necessary), and add methanol to the volume Scale, filter (0.45µm microporous membrane), take the continuous filtrate, that is, it is obtained.
[0034] 1. Chromatographic conditions
[0035] Mobile phase: A: B = acetonitrile: 0.1% phosphoric acid aqueous solution (gradient elution procedure is shown in Table 1 below), column temperature: 35 degrees, flow rate: 1.0 ml / min, wavelength: 254 nm.
[0036]
[0037] Precisely pipette the mixed reference solution (qu...
Embodiment 2
[0040] Example 2 The method of anti-rabbit erythrocyte aggregation to characterize the anti-influenza virus activity of traditional Chinese medicine for clearing heat and detoxifying
[0041] Alsever's solution: take 0.5g of citric acid monohydrate, 8.0g of trisodium citrate dihydrate, 20.5g of glucose, and 4.2g of sodium chloride, dissolve in water and dilute to 1L (about pH 6.2), and then dispense the bacteria, let it cool, and store at 4°C for later use.
[0042] Preparation of 1% erythrocyte suspension: Take 8 mL of rabbit blood, mix it with an equal volume of Ashford solution, wash 3 times with an appropriate amount of PBS, and wash with 2000 r·min for the last time. -1 Centrifuge for 10 min, take 1 mL of packed red blood cells, make 100 mL of 1% red blood cell suspension with PBS, and store at 4°C for later use.
[0043] Phosphate Buffered Saline (PBS): Take 1.10 g of anhydrous disodium hydrogen phosphate, 0.32 g of anhydrous sodium dihydrogen phosphate, and 8.50 g of ...
Embodiment 3
[0057] Example 3 The method of resisting spleen enlargement in mice to characterize the immune and anti-inflammatory activity of traditional Chinese medicines for clearing heat and detoxifying
[0058] Forty-eight SPF grade C57 female mice (13-20 g) were weighed, and their back hair was shaved with a razor to form a skin area of about 2 cm × 3 cm, and were housed in a single cage. According to body weight, they were randomly divided into 6 groups, with 8 animals in each group, namely blank group (NG), model group (MG), high-dose group (BH, 0.112g / mL), and low-dose Angelica alcohol extract. group (BL, 0.056g / mL), high-dose group of monkey earring water extract (HH, 0.256g / mL) and low-dose group of monkey earring water extract (HL, 0.128g / mL).
[0059] The blank group smeared 80 mg of Vaseline on the back of the mice regularly every day, and at the same time, the mice were gavaged with normal saline (the dosage was 0.04 mL g -1 ), the model group smeared 80 mg of imiquimod cr...
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