Full-automatic biological evaluation and chemical analysis integrated machine and method

Abstract

The invention relates to a full-automatic biological evaluation and chemical analysis integrated machine and method. The integrated machine comprises a pretreatment module used for enriching, concentrating and purifying a to-be-analyzed sample, a component separation module used for separating various compounds in a mother liquor to be analyzed, a monitoring and identifying module used for carrying out real-time monitoring and collecting of chromatographic signals and quantitative detection of suspicious compounds on effluent, a component collecting module used for collecting, decreaming, redissolving and transferring the effluent, a biological evaluation module used for finishing cell culture and detection of cytotoxicity effects and toxic targets, and a data processing and automatic control module used for collecting, sorting and analyzing comprehensive data. According to the integrated machine and method, efficient, stable and normalized standard operation conditions are provided for toxicity screening of compounds in a complex system; the reliability of experimental data is improved while the labor cost required by detection is reduced; and the method is a relatively high-practicality technical method for high-throughput screening work.

Description

technical field [0001] The invention relates to a toxicology analyzer, in particular to a fully automatic biological evaluation and chemical analysis integrated machine and method for high-throughput screening of toxic compounds, belonging to the technical field of chemical analysis and toxicity determination. Background technique [0002] The combination of chemical analysis and toxicity determination technology can effectively solve the problem of qualitative and quantitative detection of traditional toxic chemicals, but complex analysis and detection procedures and throughput bottlenecks are obstacles that need to be overcome. High-throughput screening technology is based on in vitro experimental methods at the molecular or cellular level, using microplates and liquid workstations as experimental carriers and liquid handling equipment, equipped with high-quality, high-resolution chemical analysis instruments, and analyzing the experimental results. Collect, and finally an...

AI Technical Summary

Problems solved by technology

[0004] 1) The samples of the analyzer need to be pre-treated in the laboratory before they can be loaded and separated in the component separation module; 2) The component collection module of the analyzer can only determine the components in advance through pre-experiments. The collection method and process, but there are experimental errors in the pre-experiment and the actual collection process; 3) The 96-well plate of the analyzer needs to be put back on the plate storage rack after the sample is applied, and it will be taken out manually after the overall procedure is completed for subsequent biological activities 4) The compound determination results of the analyzer and the biological detection results are independent of each other, and it is difficult to rule out the false positive phenomenon in the test results; 5) The mechanical arm of the analyzer is used as an auxiliary device It exists alone, and there is no effective integrated integration scheme established between each module, which reduces the efficiency and throughput of the overall experiment; 6) The analyzer can only partially complete the identification of pollutants in complex systems, and part of the experimental work relies on other auxiliary instruments and venue

Images