Skin care composition containing ultra-low molecular hyaluronic acid and chlorella polysaccharide and application of skin care composition

Abstract

The invention relates to a skin care composition containing ultra-low molecular hyaluronic acid and chlorella polysaccharide and application of the skin care composition. The skin care composition comprises the chlorella polysaccharide, a hamamelis virginiana extract, madecassoside, tetrahydromethylpyrimidine carboxylic acid and the ultra-low molecular hyaluronic acid. The molecular weight of the ultra-low molecular hyaluronic acid is less than or equal to 5000Da. According to the skin care composition disclosed by the invention, specific chlorella polysaccharide, hamamelis virginiana extract, madecassoside, tetrahydromethylpyrimidine carboxylic acid and ultra-low molecular hyaluronic acid are scientifically matched, so that the skin care composition has a very good synergistic effect on whitening, anti-allergy and anti-oxidation effects; the composition can have excellent whitening, anti-allergy and anti-oxidation effects. In addition, the composition provided by the invention is mild in performance, free of irritation, safe and free of toxic and side effects, and can be better applied to cosmetic products.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and in particular relates to a skin care composition containing ultra-low molecular weight hyaluronic acid and chlorella polysaccharide and an application thereof. Background Art

[0002] As people age, are affected by the external environment and the body's metabolism, many people's skin begins to show signs of aging, which in turn leads to a series of problems such as spots and dullness, as well as wrinkles and sagging skin. As people's awareness of skin care increases and they pursue beauty, more and more skin care products with anti-aging, moisturizing, whitening, anti-inflammatory and soothing effects have emerged to meet people's needs.

[0003] CN118557455A discloses a skin care composition with anti-aging effect and its application. It includes the following components in weight percentage: 10-15% of epimedium extract and 5-10% of astragaloside. The skin care composition with anti-aging effect is used in skin care products, and is applied to essence, cream, and facial mask. The application gives full play to the synergistic effect of multiple effective ingredients such as epimedium extract, astragaloside, green pod leaf extract and sand east green extract by scientifically proportioning them. These ingredients work together in multiple aspects such as anti-oxidation, anti-inflammatory, promoting skin metabolism and repair, which can comprehensively improve the skin condition and effectively delay the skin aging process.

[0004] CN115154338A discloses a highly effective anti-aging essence and a preparation method thereof, which comprises oil, surfactant, additive, benefit agent and water. The invention adopts the anti-aging composition of acetyl hexapeptide-8, hydroxypinacolone retinoic acid ester, and golden button (gum button flower / leaf / stem extract, Astragalus membranaceus root extract ubiquinone (coenzyme Q10), VE, safflower seed oil) for three-dimensional directional anti-aging; at the same time, the synergistic antioxidant formula (cellgent2020 (ethyl ferulate, rosemary extract, VE)) + VC-IP helps the epidermis to resist oxidation, and is matched with the repair combination (Xinfuyi 1609, Yuefu Ning, bisabolol, oat kernel oil) to instantly soothe and repair the barrier, and work together from the three levels of the epidermis, dermis, and muscle, focusing on the five dimensions of skin elasticity, epidermal wrinkles, contour firming, anti-oxidation and brightening, and anti-inflammatory repair, using the mutual influence between the skin cortex and the synergistic effect between active ingredients to achieve better anti-aging + antioxidant soothing and repair effects.

[0005] At present, although more active ingredients are added to the cosmetic products on the market, due to the barrier function of human skin itself, the active ingredients are difficult to be effectively absorbed by the skin, and the skin care effect often fails to achieve the expected effect. Based on this, it is necessary to provide a skin care composition with multiple functions to meet the needs of modern people. Summary of the invention

[0006] In view of the deficiencies of the prior art, the object of the present invention is to provide a skin care composition containing ultra-low molecular weight hyaluronic acid and chlorella polysaccharide and its application.

[0007] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention provides a skin care composition containing ultra-low molecular weight hyaluronic acid and chlorella polysaccharide, wherein the skin care composition comprises chlorella polysaccharide, witch hazel extract, madecassoside, tetrahydromethylpyrimidine carboxylic acid and ultra-low molecular weight hyaluronic acid;

[0009] The molecular weight of the ultra-low molecular weight hyaluronic acid is ≤5000Da, for example, it may be 500Da, 1000Da, 1500Da, 2000Da, 2500Da, 3000Da, 3500Da, 4000Da or 4500Da.

[0010] The skin care composition of the present invention selects specific chlorella polysaccharides, witch hazel extract, madecassoside, tetrahydromethylpyrimidine carboxylic acid and ultra-low molecular hyaluronic acid for scientific matching, and has a good synergistic effect in whitening, anti-allergy and anti-oxidation effects, so that the composition has excellent whitening, anti-allergy and anti-oxidation effects. Among them, chlorella polysaccharides are rich in antioxidant components, and can promote collagen synthesis, promote skin cell regeneration, and soothe the skin; hyaluronic acid can supplement the endogenous hyaluronic acid of the skin, promote the supply of skin nutrition, and by controlling its molecular weight at 5000Da, it can be better absorbed by the skin; madecassoside can inhibit the activity of glucuronidase, thereby reducing the generation and deposition of melanin, and can inhibit the activity of neutrophils and alleviate skin allergy symptoms; tetrahydromethylpyrimidine carboxylic acid has an antioxidant effect and a soothing effect, and the addition of witch hazel extract further promotes the above-mentioned effects of the composition, making it more effective in whitening, anti-allergy and anti-aging.

[0011] Preferably, the skin care composition comprises, by weight, 0.5-5 parts of Chlorella polysaccharide, 6-8 parts of Hamamelis virginiana extract, 0.1-1 parts of Madecassoside, 1-5 parts of tetrahydromethylpyrimidine carboxylic acid and 0.5-10 parts of ultra-low molecular hyaluronic acid.

[0012] The amount of the chlorella polysaccharide added to the skin care composition of the present invention can be 0.7 parts, 0.9 parts, 1 parts, 1.1 parts, 1.3 parts, 1.5 parts, 1.7 parts, 1.9 parts, 2 parts, 2.1 parts, 2.3 parts, 2.5 parts, 2.8 parts, 3 parts, 3.2 parts, 3.5 parts, 3.7 parts, 3.9 parts, 4 parts, 4.2 parts, 4.5 parts, 4.7 parts or 4.9 parts, etc.;

[0013] The amount of the witch hazel extract added can be 6.1 parts, 6.3 parts, 6.5 parts, 6.7 parts, 6.9 parts, 7.0 parts, 7.1 parts, 7.3 parts, 7.5 parts, 7.7 parts or 7.9 parts, etc.;

[0014] The added amount of madecassoside can be 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts or 0.9 parts, etc.;

[0015] The amount of the ectoine carboxylic acid added can be 1.1 parts, 1.3 parts, 1.5 parts, 1.7 parts, 1.9 parts, 2 parts, 2.1 parts, 2.3 parts, 2.5 parts, 2.8 parts, 3 parts, 3.2 parts, 3.5 parts, 3.7 parts, 3.9 parts, 4 parts, 4.2 parts, 4.5 parts, 4.7 parts or 4.9 parts, etc.;

[0016] The added amount of the ultra-low molecular weight hyaluronic acid can be 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts or 9.5 parts, etc.

[0017] Preferably, the skin care composition further comprises other plant extracts.

[0018] Preferably, the skin care composition further comprises 10-30 parts by weight of other plant extracts, for example, 11 parts, 13 parts, 15 parts, 17 parts, 19 parts, 20 parts, 21 parts, 23 parts, 25 parts, 27 parts or 29 parts, etc.

[0019] Preferably, the other plant extracts include any one of Leontopodium alpinum callus extract, Centella asiatica leaf extract, and Dendrobium officinale stem extract, or a combination of at least two thereof.

[0020] Preferably, the other plant extracts include a combination of Leontopodium alpinum callus extract, Centella asiatica leaf extract and Dendrobium officinale stem extract.

[0021] By adding the alpine edelweiss callus extract, the centella asiatica leaf extract and the dendrobium officinale stem extract to the skin care composition of the present invention, the effect of the composition in improving skin problems can be made more significant, and the present invention creatively finds that the three have more or less synergistic effects in whitening, anti-allergy and anti-oxidation.

[0022] Preferably, the mass ratio of the Edelweiss callus extract, the Centella asiatica leaf extract and the Dendrobium officinale stem extract is (0.1-1):(2-3):(0.1-1).

[0023] The first "0.1-1" can be 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8 or 0.9, etc.;

[0024] "2-3" can be 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8 or 2.9, etc.;

[0025] The second "0.1-1" can be 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8 or 0.9, etc.

[0026] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0027] In a second aspect, the present invention provides a use of the skin care composition containing ultra-low molecular weight hyaluronic acid and chlorella polysaccharide as described in the first aspect in the preparation of cosmetics.

[0028] Preferably, the mass percentage of the skin care composition containing ultra-low molecular weight hyaluronic acid and chlorella polysaccharide in the cosmetic is 1-20%, for example, it can be 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18% or 19%, etc. Other specific point values ​​within the above numerical ranges can be selected, and they will not be described one by one here.

[0029] Preferably, the cosmetics include any one of essence, essence water, lotion, facial mask or gel.

[0030] In a third aspect, the present invention provides an essence water, the components of which include the skin care composition containing ultra-low molecular weight hyaluronic acid and chlorella polysaccharide as described in the first aspect, a moisturizer, an emulsifier, a thickener and water.

[0031] Preferably, the components of the essence water include, by weight, 1-20 parts of the skin care composition containing ultra-low molecular weight hyaluronic acid and chlorella polysaccharide as described in the first aspect, 5-20 parts of moisturizer, 1-10 parts of emulsifier, 0.1-2 parts of thickener and 50-95 parts of water.

[0032] The amount of the skin care composition containing ultra-low molecular weight hyaluronic acid and chlorella polysaccharide in the essence water of the present invention can be 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts or 19 parts, etc.;

[0033] The amount of the moisturizer can be 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts or 19 parts, etc.;

[0034] The amount of the emulsifier can be 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts or 9 parts, etc.;

[0035] The amount of the thickener can be 0.2 parts, 0.3 parts, 0.1 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, 0.9 parts, 1 parts, 1.1 parts, 1.2 parts, 1.3 parts, 1.4 parts, 1.5 parts, 1.6 parts, 1.7 parts, 1.8 parts or 1.9 parts, etc.;

[0036] The amount of water can be 55 parts, 60 parts, 65 parts, 70 parts, 75 parts, 80 parts, 85 parts, 90 parts or 95 parts, etc.

[0037] Preferably, the moisturizing agent includes any one of butylene glycol, glycerin, panthenol or propylene glycol, or a combination of at least two thereof.

[0038] Preferably, the humectants are glycerin and panthenol.

[0039] The moisturizing agent in the essence water of the present invention is a combination of glycerin and panthenol, which can enable consumers to more significantly improve skin elasticity after using the essence water and achieve a better skin soothing effect.

[0040] Preferably, the mass ratio of glycerol to panthenol is 1:(0.1-1), for example, it can be 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8 or 1:0.9, etc.

[0041] Preferably, the emulsifier includes any one of dimethicone, stearyl alcohol polyether, polysorbate-20, polysorbate-60, caprylic / capric triglyceride or coconut glucoside, or a combination of at least two thereof.

[0042] Preferably, the emulsifier is dimethicone, caprylic / capric triglyceride and coco-glucoside.

[0043] The emulsifier in the essence water of the present invention is a combination of dimethicone, caprylic / capric triglyceride and coco-glucoside because the combination of the three can make the system more stable, thereby making the essence water have better antioxidant, whitening and anti-allergic effects.

[0044] Preferably, the mass ratio of dimethicone, caprylic / capric triglyceride and coco-glucoside is (1-3):(1-2):(2-3).

[0045] Wherein "1-3" can be 1.1, 1.3, 1.5, 1.7, 1.9, 2, 2.1, 2.3, 2.5, 2.7 or 2.9, etc.;

[0046] "1-2" can be 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8 or 1.9, etc.;

[0047] “2-3” can be 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8 or 2.9, etc.

[0048] Preferably, the thickener comprises any one of carbomer, xanthan gum, guar gum, cellulose gum or ammonium acryloyldimethyltaurate / VP copolymer or a combination of at least two thereof.

[0049] Preferably, the essence water also includes any one or a combination of at least two of a fragrance, a pH adjuster or a preservative.

[0050] Preferably, the fragrance comprises essence.

[0051] Preferably, the pH adjuster comprises any one of citric acid, tartaric acid, malic acid or sodium hydroxide, or a combination of at least two thereof.

[0052] Preferably, the preservative comprises ethylhexylglycerin and / or phenoxyethanol.

[0053] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0054] In a fourth aspect, the present invention provides a method for preparing the essence water as described in the third aspect, the preparation method comprising:

[0055] The essence water is obtained by homogenizing a moisturizer, an emulsifier, a thickener and a skin care composition containing ultra-low molecular weight hyaluronic acid and chlorella polysaccharide in water.

[0056] Preferably, the homogenizing step further comprises adding any one of a fragrance, a pH adjuster or a preservative, or a combination of at least two of them.

[0057] Compared with the prior art, the present invention has the following beneficial effects:

[0058] The skin care composition of the present invention selects specific chlorella polysaccharide, witch hazel extract, madecassoside, ectoine and ultra-low molecular hyaluronic acid for scientific combination, which has good synergistic effect in whitening, anti-allergy and anti-oxidation effects, and can make the composition have excellent whitening, anti-allergy and anti-oxidation effects. In addition, the composition provided by the present invention has mild performance, no irritation, safety and no toxic side effects, and will be better applied to cosmetic products. DETAILED DESCRIPTION

[0059] In order to further explain the technical means and effects adopted by the present invention, the technical solution of the present invention is further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0060] The weight percentages involved in the following embodiments and comparative examples are all calculated based on the actual effective ingredients contained in the commercially available raw materials. The commercially available raw materials may also selectively contain any one or a combination of at least two of solvents, fillers, diluents, stabilizers, pH adjusters, antibacterial agents, antioxidants or impurities within the permitted range.

[0061] The following materials and comparative examples are as follows:

[0062]

[0063]

[0064] The rest of the raw materials can be used as long as they are purchased from regular dealers.

[0065] Example 1

[0066] This embodiment provides a skin care composition containing ultra-low molecular weight hyaluronic acid and chlorella polysaccharide, wherein the skin care composition comprises, by weight, 4 parts of chlorella polysaccharide, 7 parts of witch hazel extract, 0.8 parts of madecassoside, 3 parts of tetrahydromethylpyrimidine carboxylic acid, 7 parts of ultra-low molecular weight hyaluronic acid, and 20 parts of other plant extracts;

[0067] The other plant extracts are Leontopodium alpinum callus extract, Centella asiatica leaf extract and Dendrobium officinale stem extract in a mass ratio of 1:5:1.

[0068] The preparation method of the skin care composition comprises the following steps: mixing chlorella polysaccharide, witch hazel extract, madecassoside, tetrahydromethylpyrimidine carboxylic acid, ultra-low molecular hyaluronic acid and other plant extracts to obtain the skin care composition.

[0069] Example 2

[0070] This embodiment provides a skin care composition containing ultra-low molecular weight hyaluronic acid and chlorella polysaccharide, wherein the skin care composition comprises, by weight, 3 parts of chlorella polysaccharide, 8 parts of witch hazel extract, 1 part of madecassoside, 1 part of tetrahydromethylpyrimidine carboxylic acid, 10 parts of ultra-low molecular weight hyaluronic acid, and 10 parts of other plant extracts;

[0071] The other plant extracts are Leontopodium alpinum callus extract, Centella asiatica leaf extract and Dendrobium officinale stem extract in a mass ratio of 1:2:1.

[0072] The preparation method of the skin care composition is as described in Example 1.

[0073] Example 3

[0074] This embodiment provides a skin care composition containing ultra-low molecular weight hyaluronic acid and chlorella polysaccharide, wherein the skin care composition comprises, by weight, 5 parts of chlorella polysaccharide, 6 parts of witch hazel extract, 0.5 parts of madecassoside, 5 parts of tetrahydromethylpyrimidine carboxylic acid, 5 parts of ultra-low molecular weight hyaluronic acid, and 30 parts of other plant extracts;

[0075] The other plant extracts are Leontopodium alpinum callus extract, Centella asiatica leaf extract and Dendrobium officinale stem extract in a mass ratio of 1:10:1.

[0076] The preparation method of the skin care composition is as described in Example 1.

[0077] Example 4

[0078] This embodiment provides a skin care composition containing ultra-low molecular weight hyaluronic acid and chlorella polysaccharide. The skin care composition is different from that of Example 1 only in that other plant compositions are not included in the composition. The remaining components and addition amounts are consistent with those of Example 1. The preparation method refers to that of Example 1.

[0079] Example 5

[0080] This embodiment provides a skin care composition containing ultra-low molecular weight hyaluronic acid and Chlorella polysaccharide. The difference between the skin care composition and Example 1 is that the other plant composition is an alpine edelweiss callus extract and a Centella asiatica leaf extract in a mass ratio of 1:5, and the amount of the other plant composition is kept unchanged. The other components and addition amounts are consistent with those in Example 1. The preparation method refers to Example 1.

[0081] Example 6

[0082] This embodiment provides a skin care composition containing ultra-low molecular weight hyaluronic acid and Chlorella polysaccharide. The difference between the skin care composition and Example 1 is that the other plant composition is an alpine edelweiss callus extract and a Dendrobium officinale stem extract in a mass ratio of 1:1, and the amount of the other plant composition is kept unchanged. The remaining components and addition amounts are consistent with those in Example 1. The preparation method refers to Example 1.

[0083] Example 7

[0084] This embodiment provides a skin care composition containing ultra-low molecular weight hyaluronic acid and Chlorella polysaccharide. The difference between the skin care composition and Example 1 is that the other plant composition is a Centella asiatica leaf extract and a Dendrobium officinale stem extract in a mass ratio of 5:1, and the amount of the other plant composition is kept unchanged. The remaining components and addition amounts are consistent with those in Example 1. The preparation method refers to Example 1.

[0085] Comparative Example 1

[0086] This comparative example provides a skin care composition containing ultra-low molecular weight hyaluronic acid and chlorella polysaccharide. The skin care composition is different from Example 1 only in that the components do not include North American witch hazel extract, and the reduced amount is supplemented by madecassoside, chlorella polysaccharide, tetrahydromethylpyrimidine carboxylic acid and ultra-low molecular weight hyaluronic acid in proportion. The remaining components and addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0087] Comparative Example 2

[0088] This comparative example provides a skin care composition containing ultra-low molecular weight hyaluronic acid and chlorella polysaccharide. The skin care composition is different from Example 1 only in that madecassoside is not included in the components, and the reduced amount is supplemented by chlorella polysaccharide, witch hazel extract, tetrahydromethylpyrimidine carboxylic acid and ultra-low molecular weight hyaluronic acid in proportion to the number of parts. The remaining components and addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0089] Comparative Example 3

[0090] This comparative example provides a skin care composition containing ultra-low molecular weight hyaluronic acid and chlorella polysaccharide. The skin care composition is different from Example 1 only in that tetrahydromethylpyrimidine carboxylic acid is not included in the components, and the reduced amount is supplemented by chlorella polysaccharide, North American witch hazel extract, madecassoside and ultra-low molecular weight hyaluronic acid in proportion to the number of parts. The remaining components and addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0091] Comparative Example 4

[0092] This comparative example provides a skin care composition, which is different from Example 1 only in that the composition does not include Chlorella polysaccharide, and the reduced amount is supplemented by Hamamelis virginiana extract, madecassoside, tetrahydromethylpyrimidine carboxylic acid and ultra-low molecular hyaluronic acid in proportion to the number of parts. The remaining components and the added amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0093] Comparative Example 5

[0094] This comparative example provides a skin care composition, which is different from Example 1 only in that the ultra-low molecular weight hyaluronic acid is not included in the components, and the reduced amount is supplemented by Chlorella polysaccharide, North American witch hazel extract, madecassoside and tetrahydromethylpyrimidine carboxylic acid in proportion to the number of parts. The remaining components and the added amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0095] Application Example 1

[0096] This application example provides an essence water, and its formula is as follows. The dosage unit of each component in the table is "part".

[0097]

[0098]

[0099] Its preparation method is:

[0100] (1) Heat water to 60°C, then add moisturizer, ethylhexylglycerin and thickener and stir for 20 minutes;

[0101] (2) After heating to 70°C, add the emulsifier and stir for 15 minutes;

[0102] (2) After cooling to 40° C., add the skin care composition and essence, and stir until homogeneous to obtain the essence water.

[0103] Application Example 2-7

[0104] This application example provides 6 kinds of essence waters. The components of the essence waters are different from those of Application Example 1 only in that the skin care composition prepared in Example 1 is replaced by the skin care compositions prepared in Examples 2-7 in equal amounts, and other conditions remain unchanged. The preparation method thereof refers to Application Example 1.

[0105] Application Example 8

[0106] This application example provides an essence water. The components of the essence water differ from those of application example 1 only in that the moisturizing agent is only glycerin, and the amount of the moisturizing agent remains unchanged. Other conditions remain unchanged. The preparation method thereof refers to application example 1.

[0107] Application Example 9

[0108] This application example provides an essence water, the components of which differ from those of application example 1 only in that the moisturizing agent is panthenol only, and the amount of the moisturizing agent remains unchanged, while other conditions remain unchanged. The preparation method thereof refers to that of application example 1.

[0109] Application Example 10

[0110] This application example provides an essence water, the components of which differ from those of application example 1 only in that the emulsifier is polydimethylsiloxane and caprylic / capric triglyceride in a mass ratio of 4:3, and the amount of the emulsifier is kept unchanged, while other conditions remain unchanged. The preparation method thereof refers to application example 1.

[0111] Application Example 11

[0112] This application example provides an essence water, the components of which differ from those of application example 1 only in that the emulsifier is polydimethylsiloxane and coconut glucoside in a mass ratio of 4:5, and the amount of the emulsifier is kept unchanged, while other conditions remain unchanged. The preparation method thereof refers to application example 1.

[0113] Application Example 12

[0114] This application example provides an essence water, the components of which differ from those of application example 1 only in that the emulsifier is caprylic / capric triglyceride and coconut glucoside in a mass ratio of 3:5, and the amount of the emulsifier is kept unchanged, while other conditions remain unchanged. The preparation method thereof refers to application example 1.

[0115] Comparative Application Examples 1-5

[0116] This application example provides 5 kinds of essence waters. The components of the essence waters differ from those of Application Example 1 only in that the skin care composition prepared in Example 1 is replaced by the skin care compositions prepared in Comparative Examples 1-5 in equal amounts, and other conditions remain unchanged. The preparation method thereof refers to Application Example 1.

[0117] Test Example 1 Antioxidant Efficacy Study - DPPH Free Radical Scavenging Experiment

[0118] Test sample: the skin care composition provided in Examples 1-7 and Comparative Examples 1-5;

[0119] Test method:

[0120] Accurately weigh 47.7 mg of DPPH reagent, dilute to a 100 ml brown volumetric flask, take 5 ml of it and dilute to a 50 ml brown volumetric flask with anhydrous ethanol to obtain a DPPH test solution (47.7 mg / L). Pipette 2 mL of DPPH test solution respectively, add 0.1 mL of the sample provided in Examples 1-16 and Comparative Examples 1-5 as shown in the table below, and then add an appropriate amount of anhydrous ethanol to make the total mass equal. After fully mixing, let stand in the dark for 30 min, use anhydrous ethanol as a blank control, measure its absorbance at 517 nm, and set vitamin C as a control, and calculate the scavenging rate of each extract for DPPH according to the following formula:

[0121] DPPH removal rate (%) = [1-(As-Ar) / A0] × 100%

[0122] Where: As is the absorbance of the extract and DPPH reaction system; Ar is the absorbance of the extract-anhydrous ethanol; A0 is the absorbance of the blank control anhydrous ethanol.

[0123]

[0124] The DPPH free radical scavenging rate reflects its antioxidant capacity to a certain extent. The higher the scavenging rate, the better the antioxidant degree and the better the anti-aging effect. Therefore, the anti-aging effect of the essence can be judged by studying its ability to scavenge DPPH free radicals. Vitamin C is a highly effective antioxidant recognized by the cosmetics industry. This experiment used vitamin C as a control to examine its antioxidant capacity. The test results are shown in Table 1.

[0125] Table 1

[0126]

[0127]

[0128] As can be seen from Table 1, the skin care compositions of Examples 1-3 all have better scavenging effects on DPPH, and the scavenging rates are all above 88%, which is close to that of vitamin C, while the scavenging effect of Example 4 on DPPH decreases, indicating that the addition of other plant extracts to the composition can make the antioxidant effect of the composition better. As can be seen from Examples 5-7, the type of plant extract also affects the antioxidant effect; the scavenging effect of the composition in Comparative Examples 1-5 on DPPH is significantly reduced, indicating that the four components of Chlorella polysaccharide, Hamamelis virginiana extract, madecassoside, tetrahydromethylpyrimidine carboxylic acid and ultra-low molecular hyaluronic acid promote each other in terms of antioxidant effect and have a synergistic effect.

[0129] Test Example 2 Hyaluronidase In vitro Inhibition Test

[0130] Hyaluronic acid is a component in the tissue matrix that limits the diffusion of water and other extracellular substances. After being hydrolyzed by hyaluronidase, the cells become non-sticky, causing cell degranulation and the exudation of new synthesized media, exerting biological effects and leading to the occurrence of immediate allergic reactions. Hyaluronidase is a participant in allergic reactions and has a strong correlation with allergies. Allergic phenomena can be alleviated and skin can be improved by inhibiting the activity of hyaluronidase.

[0131] Test samples: compositions provided in Examples 1-7 and Comparative Examples 1-5;

[0132] Test method:

[0133] The hyaluronidase inhibition method was used. Take 0.1mL CaCl2 solution (0.25mmol / L) and 0.5mL hyaluronidase solution (100U / mL) and react in a 37℃ water bath for 20min; add 0.5mL sample and keep warm for 20min; then add 0.5mL sodium hyaluronate solution (0.5g / L), react for 30min, then take out and cool for 5min; add 0.1mL sodium hydroxide solution (0.4mol / L) and 0.5mL acetylacetone solution (3.5mL acetylacetone dissolved in 50mL 1.0mol / L sodium carbonate solution), boil in a water bath for 15min, then immediately transfer to an ice water bath for 5min; add 1mL Ehrlich reagent (0.8g p-dimethylaminobenzaldehyde dissolved in 15mL concentrated hydrochloric acid and 15mL anhydrous ethanol), dilute with 3mL anhydrous ethanol, place at 25℃ for 20min to develop color, measure its absorbance at 540nm, and use dipotassium glycyrrhizinate as a control. The hyaluronidase inhibition rate was calculated according to the following formula:

[0134] Hyaluronidase inhibition rate = {[(A-B)-(C-D)] / (AB)}×100%

[0135] In the formula: A is the absorbance of the control solution (using acetate buffer solution instead of the sample solution); B is the absorbance of the control blank solution (using acetate buffer solution instead of the sample solution and enzyme solution); C is the absorbance of the sample solution; D is the absorbance of the sample blank solution (using acetate buffer solution instead of the enzyme solution).

[0136] Using 5% dipotassium glycyrrhizinate as a positive control, the inhibition rate of each group of samples on hyaluronidase was investigated. The results are shown in Table 2:

[0137] Table 2

[0138]

[0139]

[0140] As shown in Table 2, the skin care composition of the present invention has a good inhibitory effect on hyaluronidase, with an inhibition rate of more than 81%. Among them, the inhibition rate of Example 1 is higher than that of Example 4, indicating that the addition of other plant compositions in the composition can significantly affect the anti-allergic effect of the final product, and can make the anti-allergic effect of the composition better. As shown in Examples 5-7, the type of plant composition also affects this effect; the inhibition rate of Example 1 is higher than that of Comparative Examples 1-5, indicating that Chlorella polysaccharide, Hamamelis Virginiana extract, madecassoside, tetrahydromethylpyrimidine carboxylic acid and ultra-low molecular hyaluronic acid have a synergistic effect in anti-allergic effect, which verifies the rationality of the composition combination.

[0141] Test Example 3 Whitening effect - in vitro tyrosinase inhibition test

[0142] Preparation table 5mM L-tyrosine solution (L-tyrosine can be dissolved with a small amount of hydrochloric acid and then fixed to volume with PBS buffer) and tyrosinase solution (diluted to 100U / mL with deionized water). Use a micropipette to transfer 0.4mL of the skin care composition of Examples 1-7 and Comparative Examples 1-5, 1mL of 0.05mol / L phosphate buffer (pH=6.8) into a cuvette, then add 0.8mL of 0.5mmol / L tyrosine solution, and finally add 0.40mL of tyrosinase solution, mix quickly, and measure the growth curve of the optical density value with a wavelength of 475nm over time at a constant temperature of 30°C, and obtain the inhibition rate from the slope of the curve. Measure the inhibition rate of extracts with different concentrations respectively, draw a straight line with concentration as the horizontal axis and inhibition rate as the vertical axis, and after obtaining the linear equation, calculate the extract concentration when the inhibition rate is 50%, which is IC50. The results are shown in Table 3:

[0143] Table 3

[0144]

[0145]

[0146] As can be seen from Table 3, the skin care compositions involved in Examples 1-3 of the present invention have a good inhibitory effect on tyrosinase, and the IC50 value is below 0.6 mg / mL. Among them, the IC50 value of Example 1 is lower than that of Examples 4-7, indicating that adding a plant composition to the skin care composition can better improve the inhibitory effect of the composition on tyrosinase, and the type of plant composition also affects the inhibitory effect; the IC50 value of Example 1 is lower than that of Comparative Examples 1-5, indicating the synergistic effect of Chlorella polysaccharide, Hamamelis Virginiana extract, Madecassoside, tetrahydromethylpyrimidine carboxylic acid and ultra-low molecular hyaluronic acid in the skin care composition involved in the present invention, which verifies the rationality of the composition combination.

[0147] Test Example 4 Consumer Sensory Effect Evaluation

[0148] 102 volunteers were selected and randomly divided into 17 groups, with 6 people in each group. They tried the essence water of application examples 1-12 and comparative application examples 1-5, and then conducted sensory evaluation. The evaluation indicators were anti-allergic effect, skin elasticity and whitening effect.

[0149] Test method: The subjects used the product once in the morning and once in the evening every day, and used it for 60 consecutive days to conduct sensory evaluation on the product, using a 5-point system: 4.5 points and above, indicating very good, very satisfied; 4-4.5 points are good; 3-4 points are moderately acceptable; and below 3 points are not good, unacceptable. The scores of each group are the average of 10 people, and the results are shown in Table 4.

[0150] Table 4

[0151]

[0152]

[0153] According to Table 4, the essence water of the present invention can significantly improve the whitening and elasticity of the skin and the soothing effect by adding a specific skin care composition. The types of moisturizers and emulsifiers used in the essence water of the present invention also have a certain influence on the above effects.

[0154] Test Example 4: Safety Evaluation Experiment

[0155] This project evaluates irritation through the red blood cell hemolysis test. The red blood cell hemolysis test is one of the alternative methods to the rabbit eye irritation test (Draize test). The basic principle is to evaluate the damage of chemicals to eye tissue cells by measuring the amount of hemoglobin dissolution and the degree of denaturation. Internationally, the RBC test is mainly used to evaluate the eye irritation of chemicals such as cosmetics and raw materials. The classification standards are shown in the following table.

[0156] L / D Grading ﹥100 Non-irritating 10﹤L / D≤100 Slightly irritating 1﹤L / D≤10 Mild irritant 0.1﹤L / D≤1 Moderate irritation L / D≤0.1 Severe irritation

[0157] The results of the red blood cell hemolysis experiment on the samples of Examples 1-7 are shown in Table 5, where L / D is the ratio of HD50 (the sample concentration when 50% of the red blood cells are hemolyzed) to DI (protein denaturation index).

[0158] Table 5

[0159]

[0160]

[0161] It can be seen from Table 5 that the raw materials used in the skin care composition of the present invention are mild and non-irritating, and are relatively safe to use.

[0162] The applicant declares that the present invention uses the above-mentioned embodiments to illustrate a skin care composition containing ultra-low molecular weight hyaluronic acid and chlorella polysaccharide and its application, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0163] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0164] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A skin care composition containing ultra-low molecular weight hyaluronic acid and chlorella polysaccharide, characterized in that: The skin care composition comprises chlorella polysaccharide, witch hazel extract, madecassoside, ectoine and ultra-low molecular hyaluronic acid; The molecular weight of the ultra-low molecular weight hyaluronic acid is ≤5000Da.

2. The skin care composition containing ultra-low molecular weight hyaluronic acid and chlorella polysaccharide according to claim 1, characterized in that: The skin care composition comprises, by weight, 0.5-5 parts of chlorella polysaccharide, 6-8 parts of witch hazel extract, 0.1-1 parts of madecassoside, 1-5 parts of tetrahydromethylpyrimidine carboxylic acid and 0.5-10 parts of ultra-low molecular hyaluronic acid.

3. The skin care composition containing ultra-low molecular weight hyaluronic acid and chlorella polysaccharide according to claim 1 or 2, characterized in that: The skin care composition also includes other plant extracts; Preferably, the skin care composition further comprises 10-30 parts by weight of other plant extracts; Preferably, the other plant extracts include any one of or a combination of at least two of Leontopodium alpinum callus extract, Centella asiatica leaf extract, and Dendrobium officinale stem extract.

4. The skin care composition containing ultra-low molecular weight hyaluronic acid and chlorella polysaccharide according to claim 3, characterized in that: The other plant extracts include a combination of Leontopodium alpinum callus extract, Centella asiatica leaf extract and Dendrobium officinale stem extract; Preferably, the mass ratio of the Edelweiss callus extract, the Centella asiatica leaf extract and the Dendrobium officinale stem extract is (0.1-1):(2-3):(0.1-1).

5. Use of the skin care composition containing ultra-low molecular weight hyaluronic acid and chlorella polysaccharide as claimed in any one of claims 1 to 4 in the preparation of cosmetics.

6. The use according to claim 5, characterized in that The mass percentage of the skin care composition containing ultra-low molecular weight hyaluronic acid and chlorella polysaccharide in the cosmetic is 1-20%; Preferably, the cosmetics include any one of essence, essence water, lotion, facial mask or gel.

7. An essence water, characterized in that: The components of the essence water include the skin care composition containing ultra-low molecular weight hyaluronic acid and chlorella polysaccharide as described in any one of claims 1 to 4, a moisturizer, an emulsifier, a thickener and water.

8. The essence water according to claim 7, characterized in that The components of the essence water include, by weight, 1-20 parts of the skin care composition containing ultra-low molecular weight hyaluronic acid and chlorella polysaccharide according to any one of claims 1-4, 5-20 parts of moisturizer, 1-10 parts of emulsifier, 0.1-2 parts of thickener and 50-95 parts of water; Preferably, the moisturizing agent comprises any one or a combination of at least two of butylene glycol, glycerin, panthenol or propylene glycol; Preferably, the moisturizers are glycerin and panthenol; Preferably, the emulsifier includes any one or a combination of at least two of dimethicone, stearyl alcohol polyether, polysorbate-20, polysorbate-60, caprylic / capric triglyceride or coconut glucoside; Preferably, the emulsifier is dimethicone, caprylic / capric triglyceride and coco-glucoside; Preferably, the thickener comprises any one of carbomer, xanthan gum, guar gum, cellulose gum or ammonium acryloyldimethyltaurate / VP copolymer or a combination of at least two thereof.

9. The essence water according to claim 7 or 8, characterized in that: The essence water also includes any one or a combination of at least two of a fragrance, a pH adjuster or a preservative; Preferably, the fragrance comprises essence; Preferably, the pH adjuster comprises any one of citric acid, tartaric acid, malic acid or sodium hydroxide, or a combination of at least two thereof; Preferably, the preservative comprises ethylhexylglycerin and / or phenoxyethanol.

10. A method for preparing the essence water according to any one of claims 7 to 9, characterized in that: The preparation method comprises: The essence water is obtained by mixing a moisturizer, an emulsifier, a thickener and a skin care composition containing ultra-low molecular weight hyaluronic acid and chlorella polysaccharide in water and homogenizing them; Preferably, the homogenizing step further comprises adding any one of a fragrance, a pH adjuster or a preservative, or a combination of at least two of them.

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