Composition capable of resisting thermal stimulation and UV stimulation, application and cosmetics

Abstract

The invention belongs to the field of cosmetics, and discloses a composition which comprises a pepper fruit extract, epigallocatechin gallyl glucoside, diacetylboldine, a sanguisorba officinalis root extract and nicotinamide. The composition is detected by a DPPH method to have an excellent antioxidant effect. Meanwhile, the invention also discloses a composition capable of resisting thermal stimulation and UV stimulation, which can obviously inhibit the lipofuscin generation level of nematodes after thermal stimulation and UV stimulation by adopting nematode model detection. And after the composition is used together with the withanolaria nipponensis root extract, the composition has a remarkable synergistic effect on inhibition of lipofuscin generation of nematodes subjected to thermal stimulation and ultraviolet light stimulation. The composition provided by the invention has very good anti-wrinkle effects of light aging resistance and thermal aging resistance, and can effectively prevent the generation of color spots.

Description

Technical Field

[0001] The invention relates to the field of daily chemicals, in particular to a composition, application and cosmetics resistant to heat stimulation and UV stimulation. Background Art

[0002] Skin pigmentation is closely related to thermal and light stimulation. Improving the ability of cells to respond to thermal and light stimulation is an effective way to improve pigmentation. However, we believe that the root cause of improving skin pigmentation is not only determined by the ability of cells to respond to thermal and light stimulation, but also closely related to other factors, such as the ability to deposit and remove melanin and the ability of cells to resist oxidation. Among them, we believe that the ability of cells to resist thermal and light stimulation, and the ability to deposit and remove melanin are the basis for achieving the above-mentioned ability of cells to respond to thermal and light stimulation, and the ability to deposit and remove melanin.

[0003] The technical problem solved in this case is: how to screen out a combination with better antioxidant capacity, and how to develop anti-wrinkle cosmetic raw materials that can improve the cells' ability to respond to thermal and light stimulation. Summary of the invention

[0004] The purpose of the present invention is to provide a composition, which uses niacinamide with excellent melanin inhibition ability as a basic raw material, is combined with diacetyl boldine as a skin protectant ingredient, and uses Zanthoxylum fruit extract, epigallocatechin galloside, and Sanguisorba officinalis root extract as antioxidant synergistic ingredients, which can achieve the purpose of inhibiting melanin production and enhancing antioxidant ability, and finally achieve the effects of freckle removal, whitening, and anti-wrinkle.

[0005] At the same time, the present invention also discloses a composition that resists heat stimulation and UV stimulation. On the basis of the above composition, with the Withania somnifera root extract, the ability of cells to cope with heat stimulation and light stimulation can be effectively improved to achieve anti-wrinkle effect. In addition, the present invention also discloses the above composition and related applications of the composition containing the Withania somnifera root extract.

[0006] To achieve the above object, the present invention provides the following technical solutions: A composition comprising the following components in weight percentage: Zanthoxylum bungeanum fruit extract 0.0001~0.05wt%; Epigallocatechin galloside 0.0001~0.3wt%; Diacetyl boldine 0.0001~0.5wt%; Sanguisorba officinalis root extract 0.0005~0.5wt%; Nicotinamide 1~5wt%; The remaining amount of solvent.

[0007] The effects of each raw material of the present invention are: Skin protectant ingredient: diacetyl boldine; ingredient that inhibits melanin production: niacinamide; antioxidant active ingredients: niacinamide, Zanthoxylum bungeanum fruit extract, epigallocatechin galloside, and Sanguisorba officinalis root extract; among them, Zanthoxylum bungeanum fruit extract, epigallocatechin galloside, and Sanguisorba officinalis root extract can significantly improve the antioxidant capacity of niacinamide.

[0008] The synergistic mechanism of the antioxidant capacity of niacinamide, Zanthoxylum bungeanum fruit extract, epigallocatechin galloside, and Sanguisorba officinalis root extract is: Nicotinamide itself can neutralize the unpaired electrons of free radicals by providing hydrogen atoms, thereby reducing the damage of free radicals to cells. In addition, nicotinamide is a component of the coenzyme nicotinamide adenine dinucleotide phosphate (NADP). NADP can form reduced nicotinamide adenine dinucleotide phosphate (NADPH) through a series of enzymatic reactions in cells, which can provide electrons for antioxidant enzymes and keep them in a reduced state, thereby effectively removing free radicals.

[0009] Sanshool, the main active ingredient in Zanthoxylum bungeanum fruit extract, can reduce the content of intracellular reactive oxygen free radicals (ROS) and malondialdehyde by inhibiting the activity of oxidative stress kinase.

[0010] Epigallocatechin galloside contains multiple phenolic hydroxyl groups in its structure, which can provide hydrogen atoms to react with free radicals to achieve direct antioxidant effects; epigallocatechin galloside can chelate iron ions (Fe 3+ ) and copper ions (Cu 2+ ) and other metal ions, reducing their activity in participating in free radical generation reactions; epigallocatechin galloside can also upregulate the activity of intracellular antioxidant enzymes and regulate the redox signaling pathways in cells, thereby exerting an antioxidant effect.

[0011] The root extract of Sanguisorba officinalis mainly contains a variety of ingredients with antioxidant activity, such as tannins, flavonoids, triterpenes, etc. Its structure also has multiple phenolic hydroxyl groups and has an antioxidant mechanism similar to that of epigallocatechin galloside.

[0012] The present invention combines the above-mentioned ingredients with antioxidant effects in different dimensions, and verifies and tests the combination using the DPPH method to show that the ingredients have a good antioxidant synergistic effect.

[0013] Meanwhile, the present invention also discloses a composition for resisting heat stimulation and UV stimulation, comprising the composition as described above and Withania somnifera root extract.

[0014] The combination and the Withania somnifera root extract can synergistically improve the ability of cells to respond to heat and light stimulation. The mechanism is: Both heat stimulation and light stimulation can cause stress responses in cells. When cells are subjected to heat stimulation or light stimulation, the content of intracellular ROS usually increases, causing oxidative damage. The above-mentioned composition has excellent antioxidant effects. Analysis shows that it can reduce the production of intracellular ROS, thereby improving the ability of cells to cope with heat stimulation and light stimulation. In addition, the root extract of Withania somnifera mainly contains withanolides. Studies have shown that it can improve the adaptability and resilience of cells under heat stress, inhibit cortisol or upregulate the content of happiness factors such as dopamine, β-endorphin, 5-hydroxytryptamine, oxytocin, etc., and has a good effect in resisting heat stress.

[0015] The present invention verifies through nematode model tests that the above-mentioned composition can reduce the level of lipofuscin under thermal stimulation and ultraviolet stimulation, and after being compounded with the Withania somnifera root extract, the level of lipofuscin under thermal stimulation and ultraviolet stimulation can be further reduced, and the ability of cells to respond to thermal stimulation and light stimulation can be synergistically improved.

[0016] In the above-mentioned composition containing the Withania somnifera root extract, the weight ratio of the composition to the Withania somnifera extract is 1.0008-6.35:0.005-0.5.

[0017] In addition, the invention also discloses the use of the composition for preparing cosmetics.

[0018] Also disclosed is the use of the composition containing the Withania somnifera root extract in preparing cosmetics.

[0019] In the above-mentioned use, the cosmetics are cosmetics with the functions of resisting light aging, resisting heat aging and preventing the formation of pigment spots.

[0020] Finally, the present invention also discloses a cosmetic comprising the above composition, or the above composition containing the Withania somnifera root extract.

[0021] In the above-mentioned cosmetics, the cosmetics contain 0.1 to 30 wt % of the composition, or contain 0.1 to 30 wt % of the composition for resisting heat stimulation and UV stimulation.

[0022] Compared with the prior art, the present invention has the following beneficial effects: The present invention adopts niacinamide with excellent melanin inhibition ability as a basic raw material, combines it with diacetyl boldine, a skin protectant ingredient, and adopts Zanthoxylum bungeanum fruit extract, epigallocatechin galloside, and Sanguisorba officinalis root extract as antioxidant synergistic ingredients to achieve the purpose of inhibiting melanin production and enhancing antioxidant ability.

[0023] The present invention is based on the above-mentioned composition and is combined with Withania somnifera root extract to effectively improve the ability of cells to cope with thermal stimulation and light stimulation. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is a fluorescent photo of nematodes in the blank group in the nematode heat stimulation experiment; Figure 2 This is a fluorescent photo of nematodes in the model group during the nematode heat stimulation experiment; Figure 3 This is a fluorescent photograph of the nematode in Example 7 during the nematode thermal stimulation experiment; Figure 4 This is a fluorescent photograph of the nematode in Example 8 during the nematode thermal stimulation experiment; Figure 5 This is a fluorescent photograph of the nematode in Example 9 during the nematode thermal stimulation experiment; Figure 6 is a fluorescent photograph of the nematode in Example 10 in a nematode thermal stimulation experiment; Figure 7 is a fluorescent photograph of the nematode in Example 11 in the nematode thermal stimulation experiment; Figure 8 This is a fluorescent photograph of the nematode in Example 12 in the nematode thermal stimulation experiment; Fig. 9 is a fluorescent photograph of the nematode of Comparative Example 8 in the nematode thermal stimulation experiment; Fig.10 is a fluorescent photograph of the nematode of Comparative Example 9 in the nematode thermal stimulation experiment; Fig.11 is a fluorescent photograph of the nematode of Comparative Example 10 in the nematode thermal stimulation experiment; Fig.12 This is a fluorescent photo of nematodes in the positive group in the nematode heat stimulation experiment; Fig.13 This is a fluorescent photo of nematodes in the blank group during the nematode UV stimulation experiment; Fig.14 This is a fluorescent photo of nematodes in the model group during the nematode UV stimulation experiment; Fig.15 This is a fluorescent photograph of the nematode in Example 7 during the nematode UV stimulation experiment; Fig.16 This is a fluorescent photograph of the nematode in Example 8 during the nematode UV stimulation experiment; Fig.17 This is a fluorescent photograph of the nematode in Example 9 during the nematode UV stimulation experiment; Fig.18 is a fluorescent photograph of the nematode in Example 10 in the nematode UV stimulation experiment; Fig.19 is a fluorescent photograph of the nematode in Example 11 in the nematode UV stimulation experiment; Fig. 20 is a fluorescent photograph of the nematode in Example 12 in the nematode UV stimulation experiment; Fig.21 This is a fluorescent photo of the nematodes in Comparative Example 8 in the nematode UV stimulation experiment; Fig. 22 is a fluorescent photograph of the nematode of Comparative Example 9 in the nematode UV stimulation experiment; Fig.23 is a fluorescent photograph of the nematode of Comparative Example 10 in the nematode UV stimulation experiment; Fig.24 This is a fluorescent photo of nematodes in the positive group in the nematode UV stimulation experiment. DETAILED DESCRIPTION

[0025] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] Before describing the method of the present invention, the sources of the reagents used in the present invention are described; Zanthoxylum bungeanum fruit extract solution: sanshool content ≥ 15%; in this embodiment, the Zanthoxylum bungeanum fruit extract solution is composed of 82.5wt% oleyl alcohol and 17.5wt% Zanthoxylum bungeanum fruit extract; Epigallocatechin galloside solution: selected from the product of Givaudan with the trade name of Inoveol EGCG; in this embodiment and comparative example, the epigallocatechin galloside solution is composed of 0.75 wt % of epigallocatechin galloside and the balance of water; Diacetyl boldine solution: selected from the product named LUMISKIN MBAL of Sederma Company; in this embodiment and comparative example, the diacetyl boldine solution is composed of 0.1wt% of diacetyl boldine and the balance of caprylic / capric triglyceride; Sanguisorba officinalis root extract solution: selected from the product named Rootness Mood+ of Clariant Chemical Co., Ltd.; in this embodiment and comparative example, the Sanguisorba officinalis root extract solution is composed of 0.65 wt% of Sanguisorba officinalis root extract and the balance of 1,3-propylene glycol; The active ingredient of niacinamide is 100%; Withania somnifera root extract solution: selected from the product with the trade name of Withania somnifera Energy produced by Guangdong Heji Biotechnology Co., Ltd.; in this embodiment and the comparative example, the Withania somnifera root extract solution consists of 5.65 wt % of Withania somnifera root extract, 93.35 wt % of water and the remainder of a polyol as a solubilizer.

[0027] Ammonium acryloyldimethyltaurate / VP copolymer is a product of Clariant Chemicals Co., Ltd. under the trade name ARISTOFLEX AVC, and is used to assist in forming a more uniform mixture after compounding the composition.

[0028] Part I Preparation and Functional Verification of Compositions 1.1 Preparation of composition The formula of the composition is shown in Table 1 below; The preparation method is as follows: first weigh ammonium acryloyldimethyltaurate / VP copolymer and an appropriate amount of water, mix and heat to 70-75°C, mix evenly and then cool to room temperature, add the components of the composition in the embodiment and comparative example in sequence, add water to 100 parts by weight, and mix evenly with a homogenizer to obtain a composition sample to be tested.

[0029] Table 1 Composition formula Unit: g Test Group Zanthoxylum bungeanum fruit extract solution Epigallocatechin Gallate Glucoside Solution Diacetyl boldine solution Sanguisorba officinalis root extract solution Niacinamide Ammonium Acryloyldimethyltaurate / VP Copolymer water Example 1 0.0001 0.0001 0.0001 0.0005 1 0.1 Add to 100 Example 2 0.0001 0.0001 0.0001 0.0005 3 0.1 Add to 100 Example 3 0.0001 0.0001 0.0001 0.0005 5 0.1 Add to 100 Example 4 0.001 0.001 0.001 0.001 1 0.1 Add to 100 Example 5 0.01 0.1 0.1 0.1 3 0.1 Add to 100 Example 6 0.05 0.5 0.5 0.5 5 0.1 Add to 100 Comparative Example 1 0.0007 0 0.0001 0 1 0.1 Add to 100 Comparative Example 2 0 0.0007 0.0001 0 1 0.1 Add to 100 Comparative Example 3 0 0 0.0001 0.0007 1 0.1 Add to 100 Comparative Example 4 0.0002 0 0.0001 0.0005 1 0.1 Add to 100 Comparative Example 5 0 0.0002 0.0001 0.0005 1 0.1 Add to 100 Comparative Example 6 0.0003 0.0004 0.0001 0 1 0.1 Add to 100 Comparative Example 7 0 0 0.0001 0 1 0.1 Add to 100

[0030] 1.2 Functional verification of the composition 1.2.1 Security Testing Patch test method: Choose a suitable patch tester and use the closed patch test method. Apply about 0.03 ml of the test substance into the patch tester, and apply it to the back of the subject with special adhesive tape. Remove the test substance after 24 hours, and observe the skin reaction 0.5, 24, and 48 hours after the patch test. Record the results according to the skin reaction grading standards in the "Technical Specifications for Safety of Cosmetics" (2015 edition).

[0031] Each test sample of this test is a sample of the above-mentioned composition examples 1-6.

[0032] Test results: No adverse reactions were found.

[0033] 1.2.2 DPPH free radical scavenging experiment and results Take 2 mL of the above solution sample and mix it with 2 × 10 -4 mol / L DPPH solution was thoroughly mixed, reacted in the dark at room temperature for 30 minutes, and then the absorbance was measured at 517 nm to obtain A1; and the absorbance A2 of 2 mL of the mixed solution of the above solution sample and 2 mL of anhydrous ethanol and the absorbance A0 of the mixed solution with 2 mL of anhydrous ethanol were measured by the same operation, and the DPPH free radical scavenging rate was calculated as follows: DPPH free radical scavenging rate = [1-(A1-A2) / A0] × 100%.

[0034] The test samples of this test are the compositions in the above-mentioned Examples 1-6 and Comparative Examples 1-7.

[0035] The test results are shown in Table 2 below; Table 2 DPPH free radical scavenging test results of the composition Test Group DPPH free radical scavenging rate% Example 1 80.70 Example 2 81.13 Example 3 83.27 Example 4 90.73 Example 5 96.94 Example 6 98.41 Comparative Example 1 70.07 Comparative Example 2 72.35 Comparative Example 3 69.85 Comparative Example 4 75.95 Comparative Example 5 76.08 Comparative Example 6 78.42 Comparative Example 7 57.06

[0036] Result analysis: 1. It can be seen from Examples 1 to 3 in Table 2 that in the composition of the present invention, as the amount of nicotinamide increases, the DPPH scavenging rate increases. The reason for the limited increase is that when the amount of nicotinamide is 1%, its free radical scavenging ability is already relatively good, and the free radical scavenging ability will not increase significantly when the amount is increased.

[0037] 2. It can be seen from Examples 4 to 6 in Table 2 that as the amount of each component increases, the free radical scavenging ability increases significantly. It can be seen from Examples 1 to 6 that within the optional range of the dosage of the formula of the present invention, it can achieve a relatively excellent free radical scavenging effect.

[0038] 3. It can be seen from Example 1, Comparative Example 1, Comparative Example 2 and Comparative Example 3 in Table 2 that when the Zanthoxylum fruit extract solution, epigallocatechin galloside solution and Sanguisorba officinalis root extract solution appear in any one form, the free radical scavenging ability thereof will be reduced. Similarly, referring to Comparative Example 4, Comparative Example 5 and Comparative Example 6, it can be seen that similar situations will occur, but the reduction situation will be slightly improved.

[0039] In Comparative Example 7, when the Zanthoxylum bungeanum fruit extract solution, epigallocatechin galloside solution, and Sanguisorba officinalis root extract solution were not used, the free radical scavenging ability was significantly reduced. Since the diacetyl boldine solution did not have obvious free radical scavenging ability, it can be learned from Comparative Example 7 that niacinamide has good free radical scavenging ability when used alone.

[0040] We believe that better free radical scavenging ability is an important basis for inhibiting melanin production and improving the product's cellular heat and radiation resistance. Based on the above research, we will develop and apply subsequent compositions.

[0041] 1.2.3 Melanin production experiment and results Test sample preparation: The above composition of Example 1 was further prepared with water into a 0.2% by weight solution sample for subsequent melanin inhibition experiments (prepared and used immediately).

[0042] Melanin production test: After B16-F10 cells were plated and cultured for 16 hours, the sample solvent was added to the blank control group, melanocyte stimulating hormone (MSH) was added to the model group, MSH and kojic acid were added to the positive group, and MSH and test samples of various concentrations were added to the experimental group, and the culture was continued for 65 hours. The B16-F10 cells of different groups that had been incubated with samples for 65 hours were digested with trypsin to obtain cell suspensions, and the cell suspensions were taken for protein quantification. According to the protein quantification results, a certain amount of 1M NaOH was added to each group of sample tubes to make the total protein concentration of the solutions in each tube the same, and the absorbance was measured at 405nm.

[0043] The melanin production rate and melanin production inhibition rate were calculated as follows: Melanin production rate of model group / sample group (%) = melanin amount of model group / sample group / melanin amount of blank group × 100%; Melanin production inhibition rate (%) = (melanin production rate in model group - melanin production rate in sample group) / melanin production rate in model group × 100%.

[0044] The test results are as follows: The melanin production inhibition rate of the positive group (kojic acid) was 38.57%, and the melanin production inhibition rate of the sample group (Example 1) was 60.66%.

[0045] The concentration of Example 1 is the lowest, and its inhibitory effect on melanin production is significantly better than that of the positive group (kojic acid). This shows that the composition of the present invention has excellent melanin production inhibitory ability.

[0046] Part II Preparation and Functional Verification of Compositions Resistant to Heat and UV Stimulation 2.1 Preparation of anti-thermal and anti-UV compositions Prepare the combination of this part by referring to the preparation method of the first part; Its formula is shown in Table 3; Table 3 Formula of the composition for resisting heat stimulation and UV stimulation Unit: g Test Group Zanthoxylum bungeanum fruit extract solution Epigallocatechin Gallate Glucoside Solution Diacetyl boldine solution Sanguisorba officinalis root extract solution Niacinamide Withania somnifera root extract solution Ammonium Acryloyldimethyltaurate / VP Copolymer water Example 7 0.0001 0.0001 0.0001 0.0005 1 0.005 0.1 Add to 100 Example 8 0.0001 0.0001 0.0001 0.0005 1 0.01 0.1 Add to 100 Example 9 0.0001 0.0001 0.0001 0.0005 1 0.1 0.1 Add to 100 Example 10 0.0001 0.0001 0.0001 0.0005 1 0.5 0.1 Add to 100 Embodiment 11 0.01 0.1 0.1 0.1 3 0.1 0.1 Add to 100 Example 12 0.05 0.5 0.5 0.5 5 0.5 0.1 Add to 100 Comparative Example 8 0 0 0 0 0 0.005 0.1 Add to 100 Comparative Example 9 0.0001 0.0001 0.0001 0.0005 1 0 0.1 Add to 100 Comparative Example 10 0.0011 0.0011 0.0011 0.0025 1 0 0.1 Add to 100

[0047] 2.2 Functional verification of the composition with resistance to heat and UV Preparation of test samples: The samples of Examples 7-12 and Comparative Examples 8-10 were respectively prepared with water into 10% by weight solutions to obtain the test sample groups for subsequent nematode experiments (prepared and used immediately).

[0048] 2.2.1 Nematode thermal stimulation experiment The Caenorhabditis elegans synchronized to the L4 stage were transferred to a culture plate containing samples and 5-fluorouracil and cultured at 20°C for 4 days. After 4 days, the Caenorhabditis elegans in the sample group, positive group (curcumin) and model group were placed at 36°C for heat treatment for 3 hours, and the blank group was not heat treated. After the heat stimulation treatment, the nematodes in each group were collected and transferred to an agarose plate and anesthetized. After the nematodes were anesthetized, the fluorescence intensity was observed and photographed under a fluorescence microscope (Nikon Ti) at an excitation wavelength of 340-380nm and an emission wavelength of 430nm. The autofluorescence intensity of lipofuscin in Caenorhabditis elegans was analyzed and measured using Image J image processing software.

[0049] 2.2.2 C. elegans UV stimulation experiment The Caenorhabditis elegans synchronized to the L4 stage were transferred to a culture plate containing samples and 5-fluorouracil and cultured at 20°C for 4 days. After 4 days, the Caenorhabditis elegans in the sample group, positive group (curcumin) and model group were placed under UVA treatment with an irradiation dose of 10 j / cm² for 5 hours, and the blank group was not irradiated with UVA. After the UVA irradiation treatment, the nematodes in each group were collected and transferred to an agarose plate and anesthetized. After the nematodes were anesthetized, the fluorescence intensity was observed and photographed under a fluorescence microscope (Nikon Ti) at an excitation wavelength of 340-380nm and an emission wavelength of 430nm. The autofluorescence intensity of lipofuscin in Caenorhabditis elegans was analyzed and measured using Image J image processing software.

[0050] The inhibition rate of nematode lipofuscin production was calculated as follows: Lipofuscin production inhibition rate (%) = (1-T / C) × 100%; T: mean fluorescence intensity value of the sample group; C: Mean fluorescence intensity value of the model group.

[0051] The heat and UV resistant compositions are tested for heat and UV resistant effects as shown in Table 4 below. Table 4 Test results of the composition against heat stimulation and UV stimulation

[0052] In the accompanying drawings, Figure 1 This is a fluorescent photo of nematodes in the blank group in the nematode heat stimulation experiment; Figure 2 This is a fluorescent photo of nematodes in the model group during the nematode heat stimulation experiment; Figure 3 This is a fluorescent photograph of the nematode in Example 7 during the nematode thermal stimulation experiment; Figure 4 This is a fluorescent photograph of the nematode in Example 8 during the nematode thermal stimulation experiment; Figure 5 This is a fluorescent photograph of the nematode in Example 9 during the nematode thermal stimulation experiment; Figure 6is a fluorescent photograph of the nematode in Example 10 in a nematode thermal stimulation experiment; Figure 7 is a fluorescent photograph of the nematode in Example 11 in the nematode thermal stimulation experiment; Figure 8 This is a fluorescent photograph of the nematode in Example 12 in the nematode thermal stimulation experiment; Fig. 9 is a fluorescent photograph of the nematode of Comparative Example 8 in the nematode thermal stimulation experiment; Fig.10 is a fluorescent photograph of the nematode of Comparative Example 9 in the nematode thermal stimulation experiment; Fig.11 is a fluorescent photograph of the nematode of Comparative Example 10 in the nematode thermal stimulation experiment; Fig.12 This is a fluorescent photo of nematodes in the positive group in the nematode heat stimulation experiment; Fig.13 This is a fluorescent photo of nematodes in the blank group during the nematode UV stimulation experiment; Fig.14 This is a fluorescent photo of nematodes in the model group during the nematode UV stimulation experiment; Fig.15 This is a fluorescent photograph of the nematode in Example 7 during the nematode UV stimulation experiment; Fig.16 This is a fluorescent photograph of the nematode in Example 8 during the nematode UV stimulation experiment; Fig.17 This is a fluorescent photograph of the nematode in Example 9 during the nematode UV stimulation experiment; Fig.18 is a fluorescent photograph of the nematode in Example 10 in the nematode UV stimulation experiment; Fig.19 is a fluorescent photograph of the nematode in Example 11 in the nematode UV stimulation experiment; Fig. 20 is a fluorescent photograph of the nematode in Example 12 in the nematode UV stimulation experiment; Fig.21 This is a fluorescent photo of the nematodes in Comparative Example 8 in the nematode UV stimulation experiment; Fig. 22 is a fluorescent photograph of the nematode of Comparative Example 9 in the nematode UV stimulation experiment; Fig.23 is a fluorescent photograph of the nematode of Comparative Example 10 in the nematode UV stimulation experiment; Fig.24 This is a fluorescent photo of nematodes in the positive group in the nematode UV stimulation experiment.

[0053] Result analysis: 1. Comparative Example 8 shows that the Withania somnifera root extract solution itself has a certain ability to inhibit lipofuscin production. Comparative Examples 9 and 10 show that the inhibitory ability of the composition is slightly lower than that of the Withania somnifera extract; the two do not show significant differences.

[0054] 2. It can be seen from Examples 7, 8, 9 and 10 that as the dosage of each substance increases, its inhibitory ability is gradually improved; 3. By comparing Example 9, Example 11 and Example 10, Example 12, it can be seen that the inhibitory abilities of Example 11 and Example 12 are significantly improved. From another aspect, when the dosage of the Withania somnifera root extract solution reaches 0.5%, its performance has reached its limit. When the dosage of the composition is increased, it can improve the inhibitory ability of the composition.

[0055] Summarize: 1. The composition of the present invention has excellent melanin inhibition ability, has certain resistance to cell thermal stimulation and UV stimulation, and has relatively excellent synergy in DPPH scavenging ability, achieving the effects of freckle removal, whitening and anti-wrinkle; 2. The heat-resistant and UV-resistant composition of the present invention exhibits good resistance to cell heat stimulation and UV stimulation on the basis of having the basic functions of the above-mentioned composition. These two capabilities mean that the heat-resistant and UV-resistant composition of the present invention has better coping ability on skin aging problems such as age spots, achieving the effects of removing spots, whitening and anti-wrinkle.

Claims

1. A composition, characterized in that The composition includes the following weight percentages: Zanthoxylum bungeanum fruit extract 0.0001~0.05wt%; Epigallocatechin galloside 0.0001~0.3wt%; Diacetyl boldine 0.0001~0.5wt%; Sanguisorba officinalis root extract 0.0005~0.5wt%; Nicotinamide 1~5wt%; The remaining amount of solvent.

2. A composition for resisting heat stimulation and UV stimulation, characterized in that: The invention comprises the composition as claimed in claim 1 and Withania somnifera root extract.

3. The composition according to claim 2, characterized in that The weight ratio of the composition to the Withania somnifera extract is 1.0008-6.35:0.005-0.

5.

4. Use of the composition as claimed in claim 1 in preparing cosmetics.

5. Use of the composition according to claim 2 or 3 in preparing cosmetics.

6. The use according to claim 5, characterized in that The cosmetics are cosmetics with the functions of resisting light aging, resisting heat aging and preventing the generation of pigmentation spots.

7. A cosmetic, characterized in that: Contains the composition as claimed in claim 1, or, contains the composition as claimed in claim 2 or 3.

8. The cosmetic according to claim 7, characterized in that: The cosmetic contains 0.1 to 30 wt % of the composition according to claim 1, or contains 0.1 to 30 wt % of the composition according to claim 2 or 3.

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