15 results about "Extracellular fluid" patented technology

Provided is a structure composed of a cell population comprising endothelial cells, astrocytes and pericytes, and a 3D (three dimensional) cell growth material within which the cell population is located. The structure has a TEER value of at least 450 Ω / cm2. The cells of the structure may be derived from the brain. The cells may be human cells, and in particular may be primary derived non-immortalised cells. The structure is particularly suited for use in a model of the blood brain barrier, and the invention also provides such a model. The structure is located in a container, in which it separates a first chamber located on a first side of the structure and a second chamber located on a second side of the structure. The first and second chambers respectively contain first and second liquids in contact with first and second sides of the structure. The liquids mimic the brain extracellular fluid and the blood. The blood brain barrier model provided may be used in models of brain disease, and to investigate uptake of agents into the brain or diseased brain.

The invention discloses a central obesity early warning method and system based on BDNF dynamic monitoring, and relates to the field of dynamic monitoring of brain-derived neurotrophic factors.The method comprises the steps that the weight, the body length, the body temperature and the food intake of an experimental subject are obtained, fasting blood glucose and blood fat are detected through caudal vein blood sampling, a standardized physiological index data set is formed, and the standard physiological index data set is stored in a database; the method comprises the following steps: carrying out anatomical sampling on hypothalamus tissues, scapular brown adipose and perirenal adipose, and carrying out Western Blot detection to obtain the BDNF expression quantity of the hypothalamus tissues, the BDNF expression quantity of the scapular brown adipose tissues and the BDNF expression quantity of the perirenal adipose tissues; and acquiring the BDNF concentration time sequence data of the hypothalamus extracellular fluid through the implanted microdialysis module. According to the method, dynamic continuous monitoring of hypothalamic BDNF secretion is achieved through the implanted microdialysis technology, the rhythm disorder degree is quantified by calculating the sample entropy value, the limitation of a traditional static detection method is broken through, and early metabolic regulation abnormity can be captured more sensitively.

To provide a physical therapy for malignant tumors that is gentle to a living body, inexpensive, and highly effective.SOLUTION: Malignant cells are killed by: forming micropores in the malignant cells by shock wave irradiation, focused high-intensity ultrasound irradiation, photoimmunotherapy, or simultaneous application of microbubbles and ultrasound; and diffusing hydrogen ions, which are present in a large amount in an extracellular fluid of the malignant cells, into the malignant cells by Lorentz force.SELECTED DRAWING: None

The application relates to the technical field of electric massage, and discloses an acupoint positioning electric stimulation massage method and system based on a flexible electrode array, which comprises the following steps: pasting the flexible electrode array on a skin stimulation area, injecting a composite electric detection signal of superimposed low-frequency sine waves and high-frequency pulse sequences into the subcutaneous tissue through the electrode, collecting and extracting low-frequency impedance spectrum and high-frequency transient response waveform, determining the subcutaneous extracellular fluid resistance and fitting the capacitance charging and discharging time, combining into an acupoint electrophysiological characteristic vector, positioning the active acupoint to be stimulated through spatial clustering analysis, and then applying a biphasic asymmetric electric stimulation pulse according to the electric characteristics of the tissue, capturing an induced composite response signal in real time, fitting the density to obtain an action potential emission density trend, reducing the pulse repetition frequency and pulse width when the trend reaches a decay inflection point, and restoring the trend to a resting state level until the trend is restored to a resting state level; and the application can improve the effectiveness of electric stimulation massage.

Pharmaceutical compositions which either induce transient epithelial-mesenchymal transition (EMT) in epithelial cells, or inhibit fibroblast and immune cell migration, in the treatment of diseases or conditions associated with build-up of viscous extracellular fluids. Delivery vehicles include the use of mucus-penetrating nanoparticles

One or more electrodes for a portable bioelectric impedance monitor and methods using the monitor can measure and monitor extracellular fluid levels and / or cardiac signals using bioimpedance. The monitor may include a tetrapolar electrode array lead with four electrodes arranged sequentially and axially along the lead, and circuitry coupled with the at least four electrodes configured to measure bioelectric impedance extracellular fluid and / or cardiac signals in a human subject at various frequencies. The electrodes are adhered to a human subject / patient on the patient's torso or one of the patient's limbs.

The invention discloses a dialysis patient household dry weight management electronic scale and method based on bioelectrical impedance, and belongs to the technical field of biomedical signal detection and hemodialysis adjuvant therapy, and the method comprises the steps: collecting a multi-frequency complex impedance signal of a dialysis patient, and constructing an impedance frequency spectrum track through a Cole-Cole model based on dialysis population feature correction; self-adaptive filtering is carried out on the track in combination with a time sequence analysis technology, and high-frequency noise caused by electrode contact and electrolyte unsteady-state flow is eliminated; separating the impedance into a long-term vector drift component representing tissue mass change and a short-term modulus change component representing extracellular fluid fluctuation by using a layered decoupling algorithm; the dry tissue change and the excess moisture removal amount are calculated according to the two types of components, the dry body weight target value is calculated in combination with the current body weight, the dry body weight target value is dynamically corrected based on the substantive change of the physiological components, and therefore the risk of excessive ultrafiltration or insufficient dialysis caused by evaluation misjudgment is avoided.

A system and method for collecting plasma comprises a control circuit configured to control operation of the system, the control circuit configured to receive one or more donor parameters. The control circuit estimates a physiological fluid amount of the donor based at least in part on the one or more donor parameters and calculates a target amount of plasma product comprising raw plasma and anticoagulant by multiplying a prestored constant by the estimated physiological fluid amount. The control circuit controls the system to operate draw and return phases to process whole blood into plasma product until a measured amount of plasma product in the collection container meets the target amount of plasma product.

A wearable, non-invasive, and non-intrusive hemodynamic monitoring device is disclosed for continuous monitoring of left ventricular (LV) hemodynamic waveforms and associated cardiovascular parameters. The device includes a flexible body with laterally extending electrode wings and a central rigid housing containing signal acquisition and transmission electronics. In various embodiments, the device includes either two or three wings configured for placement along the midsternal region and over the carotid arteries to enable simultaneous acquisition of electrical and mechanical signals via bioimpedance, ECG / EKG, and accelerometer sensors. Each wing incorporates tetrapolar bioimpedance electrode arrays, current injection electrodes, voltage sensing electrodes, and accelerometers to capture thoracic and cervical physiological signals necessary for estimating parameters such as left ventricular end diastolic pressure, extracellular fluid, left ventricular ejection fraction, cardiac out, stroke volume and QRS measures. The sensor signals are routed through flexible interconnects or magnetic mating connectors to the central housing. The system includes a wireless transmitter module supporting Bluetooth, Wi-Fi, or other wireless standards, a power supply and power management circuitry, and is constructed on ultra-thin, stretchable substrates composed of biocompatible flexible polymers. The modular design also includes user-serviceable magnetic connectors and optional adhesive layers for improved skin contact, thereby enabling continuous, real-time cardiovascular monitoring suitable for ambulatory and remote healthcare environments.

A wearable, non-invasive, and non-intrusive hemodynamic monitoring device is disclosed for continuous monitoring of left ventricular (LV) hemodynamic waveforms and associated cardiovascular parameters. The device includes a flexible body with laterally extending electrode wings and a central rigid housing containing signal acquisition and transmission electronics. In various embodiments, the device includes either two or three wings configured for placement along the midsternal region and over the carotid arteries to enable simultaneous acquisition of electrical and mechanical signals via bioimpedance, ECG / EKG, and accelerometer sensors. Each wing incorporates tetrapolar bioimpedance electrode arrays, current injection electrodes, voltage sensing electrodes, and accelerometers to capture thoracic and cervical physiological signals necessary for estimating parameters such as left ventricular end diastolic pressure, extracellular fluid, left ventricular ejection fraction, cardiac out, stroke volume and QRS measures. The sensor signals are routed through flexible interconnects or magnetic mating connectors to the central housing. The system includes a wireless transmitter module supporting Bluetooth, Wi-Fi, or other wireless standards, a power supply and power management circuitry, and is constructed on ultra-thin, stretchable substrates composed of biocompatible flexible polymers. The modular design also includes user-serviceable magnetic connectors and optional adhesive layers for improved skin contact, thereby enabling continuous, real-time cardiovascular monitoring suitable for ambulatory and remote healthcare environments.

Provided herein are dynamic clusters of atom particles activated in extracellular fluid intended to reverse the accumulation of advanced glycation end products which are closely related to chronic diseases. The manufacturing process involves material preparation, particle fragmentation, pH adjustment, selection of atom particles and construction of dynamic clusters through electromagnetic field and flow velocity adjustment, and separation of dynamic clusters from the liquid. The dynamic clusters of atom particles deal with advanced glycation end products, which are diverse in chemical nature, by responding to irregularities in the electromagnetic field disrupted by highly reactive free radicals advanced glycation end products emit. Such mechanism makes it possible to extend the applications to diseases related to irregular free radical activities other than chronic diseases like in HIV replication or neural signalling.

The invention provides a yak meat fresh-keeping treatment method capable of intelligently regulating and controlling a high-voltage pulse electric field, and relates to the technical field of food processing.The yak meat fresh-keeping treatment method comprises the steps that a dielectric spectrum sensor is used for monitoring the conductivity value of extracellular fluid in real time, the field intensity of a composite waveform high-voltage pulse electric field is adjusted based on the conductivity value, and the yak meat fresh-keeping treatment method is obtained through the composite waveform high-voltage pulse electric field; accelerating the precursor loading liquid to enter muscle fibers and generate oxidative polymerization to form an endogenous preservative, and spraying a nanocrystallized natural antibacterial peptide solution, so that the antibacterial peptide instantly enters cytoplasm to deeply kill by virtue of an electroporation channel, and a layer of physical exogenous protective agent barrier is formed on the surface of the meat product; according to the method, yak meat is taken as a raw material, after-ripening maintenance is performed in a low-temperature and high-humidity environment of a high-voltage electrostatic field, storage and transportation are performed in cooperation with directional modified atmosphere packaging, a four-in-one long-acting fresh-keeping system with an internal chemical barrier, a surface biological barrier, an external physical barrier and a whole-course low-temperature barrier is formed, and stable, controllable and long-acting maintenance of the yak meat quality is achieved.

The invention discloses a bio-impedance extraction method based on improved M estimation and IRLS, and relates to the technical field of bio-impedance extraction, the method is an improved M estimation and iterative reweighted least square fitting IRLS algorithm, the method comprises the following steps: firstly, carrying out prior processing on acquired data, then carrying out weight assignment according to the characteristics of each frequency point, and finally, extracting the frequency point according to the weight assignment; and finally, an accurate circle center point and a radius are extracted, so that accurate intracellular and extracellular fluid impedance values in the human body biological impedance model are obtained. According to the method, the problems that in the process that the Cole-Cole model is used for solving the biological impedance of the human body, the requirement for the quality of collected signals is high, and the influence of noise data on the final result during fitting is large are solved, and accurate extraction of the biological impedance is achieved.

This invention proposes a plating method to improve the stability of hERG-HEK293 cells during patch-clamp assays, comprising the following steps: S1, placing cell slides in a culture dish, coating them with matrix gel for 10-30 min, and then aspirating the coating solution; S2, adding hERG-HEK293 cell suspension to the matrix gel-coated cell slides prepared in step S1, incubating in an incubator for 5-20 min, and then adding culture medium to cover the slides for cell culture; S3, removing the cell slides from the culture dish and placing them in a culture dish containing extracellular fluid, then removing the slides and placing them in a chamber filled with extracellular fluid for patch-clamp assays. This invention uses matrix gel to coat the cell slides, increasing cell stability, ensuring strong cell adhesion, reducing the risk of cell washout during perfusion, improving the cell perforation rate, and extending the cell recording time.

A portable bioelectric impedance monitor and methods using the monitor can measure and monitor extracellular fluid levels and / or cardiac signals. The monitor may include a tetrapolar electrode array lead with four electrodes arranged sequentially and axially along the lead, and circuitry coupled with the at least four electrodes configured to measure bioelectric impedance extracellular fluid and / or cardiac signals in a human subject at various frequencies. The electrodes are adhered to a human subject / patient on the patient's torso or one of the patient's limbs. One embodiment includes a Tetrapolar Analog Front End Patient Interface circuit configured to convert two electrode operation of a commercial Impedance Converter, Network Analyzer into a tetrapolar operation for excitation and impedance measurement of the human subject.