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Preparation method of microbe immobilized particles for restoring pesticide contaminated soil

A technology for microbial immobilization and pesticide-contaminated soil is applied in the field of in-situ remediation of pesticide-contaminated soil to achieve the effects of high bacterial count, good ventilation and low price.

Inactive Publication Date: 2010-05-05
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the application of immobilized microorganism technology to the remediation of non-fluid media (such as soil) polluted by organic matter has not been reported at home and abroad.

Method used

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  • Preparation method of microbe immobilized particles for restoring pesticide contaminated soil
  • Preparation method of microbe immobilized particles for restoring pesticide contaminated soil
  • Preparation method of microbe immobilized particles for restoring pesticide contaminated soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] See attached Figure 5 , (1) Crushing and screening of carbon blocks:

[0043] Break the large pieces of charcoal, and sieve out small particles with a diameter of 0.80mm and a specific surface area of ​​1155m 2 / g, pore volume 0.86cm 3 / g. Then the charcoal particles were aerated in distilled water for 5 hours to remove the charcoal powder and dust attached to the surface, and dried for later use. figure 1 , 2 is an electron micrograph of the charcoal particles.

[0044] (2) Bacterial slant culture:

[0045] Pseudomonas (Pseudomonas sp .) Placed in a 28-30°C incubator for 48 hours on a slant.

[0046] (3) Preparation of bacterial seed solution:

[0047] Receive 2 rings of bacterial lawn on the slant medium with an inoculation loop, insert into the liquid seed medium (its component weight ratio is: glucose 1.25%, beef extract 0.25%, NH 4 NO 3 0.1%, MgSO 4 ·7H 2 O 0.02%, KCl 0.02%, pH 7.0-7.2), under the conditions of shaking table speed 130-150r / min, 28-30 ...

Embodiment 2

[0062] The difference from Example 1 is:

[0063] The carrier material is bamboo charcoal particles with a particle size of 1.60mm and a specific surface area of ​​860m 2 / g, pore volume 0.77cm 3 / g.

[0064] The bacterial species is Bacillus sp.

[0065] The crosslinking agent is an aqueous solution of carboxymethyl cellulose with a mass concentration of 3%.

[0066] The primary reinforcing agent is an aqueous solution of chitosan with a mass concentration of 1.5%, and the secondary reinforcing agent is an aqueous solution of sodium alginate with a mass concentration of 2.5%.

[0067] The immobilized particle dosage ratio is 5%, when the initial concentration of 2,4-D in the soil is 40mg / kg, the removal rate of 2,4-D is 42% after repairing for 35 days.

Embodiment 3

[0069] The difference from Example 1 is:

[0070] The carrier material is coconut shell-based granular carbon with a particle size of 0.80mm and a specific surface area of ​​1104m 2 / g, pore volume 0.82cm 3 / g.

[0071] The bacterial species is Micrococcus sp.

[0072] The crosslinking agent is an aqueous solution of 4% carboxymethyl cellulose in mass concentration.

[0073] The primary reinforcing agent is an aqueous solution of chitosan with a mass concentration of 2%, and the secondary reinforcing agent is an aqueous solution of sodium alginate with a mass concentration of 2%.

[0074] The immobilized particle dosage ratio is 2%, when the initial concentration of bensulfuron-methyl in the soil is 20mg / kg, the removal rate of bensulfuron-methyl is 62% after 28 days of restoration.

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Abstract

The invention relates to in-situ restoration of pesticide contaminated soil, in particular to a preparation method of microbe immobilized particles for restoring pesticide contaminated soil. Porous carbon is used as a main carrier material, and cellulose, chitosan and sodium alginate are used as auxiliary carriers to realize the purpose of immobilizing the microbes; and the obtained microbe immobilized particles are used for in-situ restoration of the pesticide contaminated soil. The carrier material used in the invention has cheap price, low cost, wide sources, good air permeability and no toxicity to organisms, and can not cause secondary pollution to the soil. The carrier and the immobilizing process thereof can be used for immobilizing multi-bacterium microbes, such as pseudomonas, bacillus, zoogloea, micrococcus and the like, and have certain broad-spectrum applicability; and the bacillus can reach more than 10 billions / gram. Compared with the prior immobilizing method, the invention is characterized by directly sowing the immobilized cell particles into the soil without destroying the original soil structure.

Description

【Technical field】 [0001] The present invention relates to the in-situ remediation of pesticide-contaminated soil, specifically a method for preparing microorganism-immobilized particles for the remediation of pesticide-contaminated soil, that is, using porous carbon as the main carrier material to achieve the immobilization of microorganisms, and the obtained microorganisms Immobilized particles for in-situ remediation of pesticide-contaminated soil. 【Background technique】 [0002] As an important means to ensure a good agricultural harvest, chemical pesticides play a very important role in agricultural production. However, due to the lack of science in the long-term application of pesticides, especially the large-scale use of highly toxic, high-residue, and refractory pesticides, the amount of pesticides in the environment far exceeds the self-purification capacity of the environment, that is, exceeds the capacity of the environment. We are facing the environmental problem...

Claims

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Application Information

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IPC IPC(8): C12N11/14B09C1/10A62D3/02A62D101/04
Inventor 司友斌张进霞司雄元彭军
Owner ANHUI AGRICULTURAL UNIVERSITY
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