Composite microbial inoculant for degrading aflatoxin B1 and preparation method thereof

A technology of aflatoxin and compound bacterial agent, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of raw material destruction, secondary pollution, carcinogenicity, etc., and achieve efficient and rapid degradation, low cost, No secondary pollution effect

Active Publication Date: 2016-09-07
FARM PROD PROCESSING & NUCLEAR AGRI TECH INST HUBEI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aflatoxin can reduce the palatability and nutritional value of feed, affect the reproductive function of animals, lead to disorder of animal reproductive function, and also has immunotoxicity and liver toxicity, which can cause a large number of livestock and poultry deaths and strong carcinogenic effects, and even affect humans. healthy
[0004] Existing physical and chemical methods to remove aflatoxin B1 often require high costs, and are likely to cause damage to raw materials and secondary pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0021] A preparation method for a compound bacterial agent for degrading aflatoxin B1, comprising:

[0022] S1. Cultivate Pseudomonas, Flavobacterium, Rhodococcus, Stenotrophomonas and Bacillus respectively to obtain Pseudomonas bacterial liquid, Flavobacterium bacterial liquid, Rhodococcus bacterial liquid, Stenotrophomonas bacterial liquid solution and Bacillus bacteria solution.

[0023] Among them, Pseudomonas sp. comes from strain preservation number CICC 23439, Flavobacterium sp. comes from strain preservation number CICC 20907, Rhodococcus sp. comes from strain preservation number DSM 763, Stenotrophomonas (Stenotrophomonas sp.) from strain deposit number DSM 21257, and Bacillus sp. from strain deposit number ATCC21228.

[0024] Specifically, Pseudomonas and Flavobacterium were inserted into Pseudomonas culture medium and Flavobacterium culture medium respectively, and cultured at a temperature of 27-32° C. and aerobic conditions for 45-50 hours. Wherein, the aerobic ...

Embodiment 1

[0039] In the present embodiment, a kind of preparation method that is used to degrade the compound bacterial agent of aflatoxin B1 comprises:

[0040]First, insert Pseudomonas bacteria from the strain preservation number CICC 23439 into a Pseudomonas culture medium consisting of 4 g of peptone, 3 g of beef extract, 5 g of sodium chloride, 5 mg of manganese sulfate, and 1000 mL of distilled water. 7.0, cultured for 48 hours at a temperature of 28°C and under aerated conditions.

[0041] Insert the Flavobacterium from the strain preservation number CICC 20907 into the Flavobacterium medium consisting of 5g of peptone, 3g of beef extract, 5g of sodium chloride, 5mg of manganese sulfate and 1000mL of distilled water, at a pH of 7.1 and a temperature of Incubate with shaking at 30°C for 48 hours.

[0042] The rhodococcus from the strain preservation number DSM 763 was inserted into a rhodococcus culture medium consisting of 3 g of glucose, 3 g of yeast powder, 9 g of malt extract...

Embodiment 2

[0055] In the present embodiment, a kind of preparation method that is used to degrade the compound bacterial agent of aflatoxin B1 comprises:

[0056] First, insert Pseudomonas bacteria from the strain preservation number CICC 23439 into a Pseudomonas culture medium consisting of 6 g of peptone, 4 g of beef extract, 6 g of sodium chloride, 6 mg of manganese sulfate, and 1050 mL of distilled water. 7.2, the temperature was 30°C and the condition of ventilation was cultivated for 50 hours.

[0057] Insert the Flavobacterium from the strain preservation number CICC 20907 into the Flavobacterium medium composed of 5g of peptone, 4g of beef extract, 4g of sodium chloride, 5mg of manganese sulfate and 1000mL of distilled water, at a pH value of 6.9 and a temperature of Incubate with shaking at 29°C for 48 hours.

[0058] The rhodococcus from strain preservation number DSM 763 was inoculated into a rhodococcus medium consisting of 5 g of glucose, 5 g of yeast powder, 11 g of malt e...

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Abstract

The invention discloses a preparation method of a composite microbial inoculant for degrading aflatoxin B1 and a composite microbial inoculant prepared by the method. The method comprises the following steps: respectively culturing pseudomonad, Flavobacterium, Rhodococcus, Stenotrophomonas and Bacillus to obtain a pseudomonad solution, a Flavobacterium solution, a Rhodococcus solution, a Stenotrophomonas solution and a Bacillus solution; mixing the pseudomonad solution, Flavobacterium solution, Rhodococcus solution, Stenotrophomonas solution and Bacillus solution to obtain a composite bacterium solution; and putting the composite bacterium solution into a composite bacterium culture medium, and carrying out mixed culture fermentation to obtain the composite microbial inoculant for degrading aflatoxin B1. The composite microbial inoculant can efficiently degrade aflatoxin B1, and has the advantages of low cost and no secondary pollution.

Description

technical field [0001] The invention relates to the technical field of aflatoxin degradation, in particular to a composite microbial agent for degrading aflatoxin B1 and a preparation method thereof. Background technique [0002] Aflatoxins are toxic metabolites produced by Aspergillus flavus and Aspergillus parasiticus. In nature, the growth requirements of Aspergillus flavus are not high. Under aerobic conditions, peanuts and corn are the best breeding places. During the storage and transportation of feed materials (such as corn, wheat, rice), etc., when the conditions are suitable (such as temperature 25 ℃ ~ 30 ℃, moisture content 17% ~ 20%), it is very easy to breed Aspergillus flavus and produce Aspergillus flavus Toxin pollution. [0003] Aflatoxin is an unavoidable and extremely harmful contamination in feed production, and it is difficult to remove. However, aflatoxins (especially aflatoxin B1) have strong toxic effects on most animals. Aflatoxin can reduce the p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/38C12R1/20C12R1/07C12R1/01
CPCC12N1/20
Inventor 郭鹏王轶程薇
Owner FARM PROD PROCESSING & NUCLEAR AGRI TECH INST HUBEI ACAD OF AGRI SCI
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