14 results about "Flavobacterium sp." patented technology

The invention belongs to the technical field of biological agents, and particularly relates to ginsenoside variant flavobacterium DG-16 and application thereof in relieving continuous cropping obstacles of angelica sinensis and improving the quality of the angelica sinensis. According to the ginsenoside variant flavobacterium DG-16 disclosed by the invention, the growth of angelica sinensis is promoted by increasing the contents of jasmonic acid and cytokinin hormones, reducing the contents of gibberellin and salicylic acid hormones and promoting the circulation and utilization of nitrogen; in addition, the flavobacterium ginsenoside variant DG-16 can significantly increase the chlorophyll content, is beneficial to photosynthesis of the angelica sinensis, effectively changes the physiological and biochemical states of the angelica sinensis, can resist root rot of the angelica sinensis so as to relieve successive cropping obstacles and improve the quality, and has a significant effect.

The invention provides a novel ecological feed capable of preventing multiple grass-bream diseases, the novel ecological feed comprises a feed matrix and an ecological feed leavening agent, and the ecological feed leavening agent comprises probiotics with surface expression antigens, the antigen is any one or more of a grass carp reovirus antigen, a flavobacterium johnsonii antigen, an aeromonas veronii antigen and a clonorchis sinensis antigen. The novel ecological feed with a disease-resistant function in a parabramis pekinensis breeding mode is prepared for the first time, the resistance of the parabramis pekinensis to various pathogens can be enhanced, and the survival rate of the parabramis pekinensis in the disease attack peak period can be increased by 50% or above after immunization. An oral immunization mode is adopted and can be completed in the feeding process, and the immunization and breeding cost is greatly saved.

According to the genetically engineered bacterium for high yield of p-coumaric acid and the application of the genetically engineered bacterium, tyrosine ammonia lyase from different sources and expression vectors with different copy numbers are combined to optimize metabolic pathways, so that the yield of p-coumaric acid is increased. The method specifically comprises the following steps: constructing recombinant expression vectors by using tyrosine ammonia lyase FjTAL from flavobacterium johnsonii and tyrosine ammonia lyase FsTAL from flavobacterium cissii and expression vectors pACYCDuet-1, pCDFDuet-1, pETCDuet-1 and pRSFDuet-1 with different copy numbers respectively by using a homologous recombination technology, transferring the recombinant expression vectors into escherichia coli BL21 (DE3) to obtain engineering strains pCA1-pCA8, and carrying out fermentation on the engineering strains pCA1-pCA8 to obtain the tyrosine ammonia lyase FjTAL from flavobacterium johnsonii and the tyrosine ammonia lyase FsTAL from flavobacterium cissii. Glucose is used as a substrate for fermentation and screening to obtain the high-yield engineering bacterium pCA8, and the yield of p-coumaric acid of the engineering bacterium pCA8 reaches 251.61 mg / L. By optimizing key enzyme expression, tyrosine is efficiently promoted to be converted into p-coumaric acid, and the method has the advantages of being low in cost, high in efficiency, environmentally friendly and the like, is suitable for industrial production and can be widely applied to the fields of biomedicine, food, cosmetics and the like.

The invention belongs to the technical field of sewage treatment, and particularly relates to a preparation method of a low-temperature composite flora, a cold-resistant microbial agent for low-temperature sewage treatment and application of the cold-resistant microbial agent. The invention provides a low-temperature complex microbial community, which is prepared from flavobacterium (Flavobacterium), aeromonas (Aeromonas), sphingomonas (Sphingobacterium), pseudomonas (Pseudomonas), Comamonas (Comamonas), Massiella (Massiella) and Delftia (Delftia), and is characterized in that the low-temperature complex microbial community is prepared from the following raw materials: the flavobacterium (Flavobacterium), the aeromonas (Aeromonas), the sphingomonas (Sphingobacterium), the pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas The low-temperature composite flora can treat domestic wastewater under a low-temperature condition, and is high in nitrogen removal rate, short in treatment time, wide in application range and better in impact resistance.

The application provides application of a broadleaf paloontia endophytic flavobacterium in promoting plant growth, the flavobacterium strain P1R7GS3 in the application is separated from the roots of healthy broadleaf paloontia and is preserved in the Guangdong Microbial Digital Culture Collection Center with a preservation number of GDMCC No: 66990; has the effects of ammonia production, indole acetic acid production and zinc dissolution and has a biological membrane formation capacity, which has an excellent promoting effect on the growth of plants, in particular, the growth of broadleaf paloontia. The application can significantly promote the growth of stem thickness, leaf number, fresh weight, leaf fresh weight, dry weight and leaf dry weight of broadleaf paloontia plants; meanwhile, the application promotes the synthesis of effective substances such as broadleaf paloontia alcohol and broadleaf paloontia ketone while improving the biomass of broadleaf paloontia; and can be used as a biological fertilizer for promoting the growth of field crops and effectively reducing the use of chemical fertilizers.

The invention discloses a method for producing polyhydroxyalkanoate through microbial fermentation and application of the polyhydroxyalkanoate. The method for producing the polyhydroxyalkanoate through microbial fermentation comprises the following steps: optionally adding water into organic acid feed liquid for dilution, and then adding a complex microbial inoculant containing flavobacterium for aerobic fermentation to obtain PHA-rich fermentation thalli; the complex microbial inoculant comprises neosilurus meridionalis, nitrogen-fixing vibrio, dearomatization bacteria, flavobacterium and paracoccus; the organic acid feed liquid is obtained by carrying out acid production fermentation on a pretreated material derived from lignocellulose. According to the method, the lignocellulose is used as a raw material to replace a grain raw material to produce the organic acid under the condition of no enzymolysis. The microbial agent disclosed by the invention contains neosilurus meridionalis, dearomatization bacteria, flavobacterium, flavobacterium and paracoccus, the strains act together, the organic acid can be effectively utilized for further fermentation to generate polyhydroxyalkanoate, and the microbial agent has important economic and environmental benefits.

This invention discloses a strain of *Flavobacterium huinanense*, an inoculant, and its application in the control of potato scab, belonging to the field of green control technology for potato scab. This invention isolates a strain of *Flavobacterium huinanense* from the soil surface of potatoes. Flavobacterium anhuiense R10, which has a significant antagonistic effect on *Streptomyces bozzolani*, the pathogen of potato scab. *Flavobacterium huinanense* ( Flavobacterium anhuiense The fermentation broth of R10, when used for root irrigation of potatoes, can significantly reduce the incidence and severity index of potato scab.

The invention discloses flavobacterium anhuiense, a fungicide and application of the flavobacterium anhuiense to prevention and control of potato scab, and belongs to the technical field of green prevention and control of potato scab. According to the invention, a strain of Flavobacterium anhuiense R10 is separated from soil on the surface of the potato, and the strain of Flavobacterium anhuiense R10 has a remarkable antagonistic effect on a potato scab pathogenic bacterium, namely, streptomyces botroticus. According to the present invention, the root irrigation treatment is performed on the potatoes by using the fermentation broth of the Flavobacterium anhuiense R10, such that the disease grade and the disease index of the potato scab can be significantly reduced;

The present invention provides: a novel allulose epimerase variant in which an amino acid residue present at a specific position of an amino acid sequence of a wild-type D-allulose 3-epimerase derived from Flavonifractor plautii is substituted with another amino acid residue; and various uses thereof. The novel allulose epimerase variant according to the present invention has significantly higher conversion activity of fructose to allulose than a wild-type D-allulose 3-epimerase derived from Flavonifractor plautii or a D-allulose epimerase variant W29K / A77S / G216S / M234I. Therefore, the novel allulose epimerase variant according to the present invention can shorten production time and reduce production costs in an enzyme conversion reaction at an industrial scale for mass production of allulose.

This invention provides a strain of Flavobacterium ZJUHULHQ1 and its application in controlling *Pseudomonas gramineus* root-knot nematodes, belonging to the field of biological control technology for root-knot nematodes. The Flavobacterium ZJUHULHQ1 is deposited at the China Center for Type Culture Collection (CCTCC), accession number CCTCC NO: M 20251179. The Flavobacterium ZJUHULHQ1 can enhance plant resistance by increasing the content of the defense hormones salicylic acid, jasmonic acid, and jasmonic acid-isoleucine in rice, thereby inhibiting the invasion of *Pseudomonas gramineus* root-knot nematodes and reducing the number of root knots. Simultaneously, the Flavobacterium ZJUHULHQ1 has a certain growth-promoting effect on rice and has broad application prospects.

The invention provides a microbial agent for preventing and treating soybean soil-borne diseases and application of the microbial agent, and belongs to the technical field of agricultural biology. The microbial agent is prepared by compounding a bacterial strain RHEr1 (Enterobacter rogengenkampii), a bacterial strain RHCc2 (Chryseobacterium cucumerii), a bacterial strain RHPc03 (Pseudomonas chlororaphis) and a bacterial strain RHBs9 (Bacillus subtilis), and the ratio of viable counts of the bacterial strains is (20 to 28): (22 to 30): (18 to 24): (5 to 9). The microbial agent effectively prevents and treats soybean root rot caused by fusarium, rhizoctonia and the like through multiple antagonism, nutrition competition and an induction system resistance mechanism, has the functions of nitrogen fixation, phosphate solubilization and auxin secretion, and remarkably promotes the growth of soybean plants. The preparation is environment-friendly, has no chemical residue, is not easy to cause drug resistance, and is suitable for green and sustainable soybean production.

The application provides a method for promoting the propagation of Stephanodinium williamsii and a microorganism used in the method, wherein the microorganism is a strain of Flavobacterium sp. Flagellimonas FLG-A, and the preservation number of the strain is CGMCC No. 34666. The Flavobacterium sp. FLG-A provided by the application can effectively improve the propagation effect of the Stephanodinium williamsii, and has a good production application prospect.

The invention discloses a method for producing PHA through fermentation of organic acid mixed liquor based on a cellulose source and application. The method comprises the following steps: adding a complex microbial inoculant into an optional organic acid mixed solution which is diluted or not diluted with water, and carrying out aerobic fermentation, so as to obtain a PHA-rich zymophyte; raw materials including cellulose are subjected to acid production fermentation, and the organic acid mixed liquid is obtained. It is found for the first time that new silurus meridionalis, dearomatization bacteria and flavobacterium can produce PHA through metabolism by using organic acid as a carbon source and are matched with flavobacterium and paracoccus to prepare the complex microbial inoculant, and the complex microbial inoculant can be used for fermenting an organic acid mixed solution based on a cellulose source to produce PHA, so that the yield of PHA is effectively increased, and the production cost is reduced.

The invention discloses bacillus velezensis, a culture and application of the bacillus velezensis to prevention or treatment of aquatic animal diseases, and relates to the cross technical field of biotechnology and aquatic disease prevention and control. The preservation number of the bacillus velezensis is CCTCC (China Center For Type Culture Collection) NO: M 20252822, and the bacillus velezensis is classified and named as bacillus velezensis LB7. The bacillus velezensis has broad-spectrum antibacterial activity and can destroy biofilms of pathogenic bacteria. Through culture medium optimization, the bactericidal activity of bacillus velezensis can be further improved. The culture supernatant of the bacillus velezensis LB7 is mixed with feed to feed grass carps infected with flavobacterium clostridium, so that the survival rate of the grass carps can be remarkably increased, and a treatment effect is achieved. The fermentation product provided by the invention has remarkable sterilization and bacteriostasis effects, and can be used as a synergist of antibiotics florfenicol and enrofloxacin, so that the investment of the two antibiotics in aquaculture is reduced.