Microbial preparation for improving aquatic product culture environment and preparation method thereof

A microbial preparation and aquaculture technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of biological bactericides that have not been found yet, achieve good production and application prospects, simple preparation methods, and easy decomposition effect of effect

Active Publication Date: 2015-04-08
福建汇盛生物科技有限公司
View PDF7 Cites 23 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, it is generally believed in the world that biological control may be one of the more effective ways to solve aquaculture diseases, but there is no biological fungicide specific

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0045] And the specific operation steps of the microbial preparation preparation method are as follows:

[0046] (1) Cultivation of Rhodopseudomonas palustris strain PSB20: Take Rhodopseudomonas palustris strain PSB20 and inoculate it into medium Ⅰ, and culture it at 25-35°C and 2500-3500lx under light for 72-144h, When the OD of the culture medium 660 When the seed liquid reaches 1.5-2.2, the seed liquid is prepared; then the seed liquid is inoculated to transparent plastic agricultural film according to the inoculum amount of 5-20%, and cultivated at 30-35 °C for 3-5 days under sunlight, and the cloudy, And at night, use 60W / 25L incandescent lamp to irradiate, when the bacterial liquid turns deep red, the cultivation is completed;

[0047] (2) Concentration and solidification of Rhodopseudomonas palustris strain PSB20: add a flocculant to the cultured bacterial liquid obtained in step (1), let stand overnight to flocculate, and remove the supernatant; then add the protecti...

Embodiment 1

[0055] Cultivation of strain PSB20: take strain PSB20 and inoculate it into medium Ⅰ, and culture it under light at 27°C and 2500lx for 72h, when the OD of the culture medium is 660 When the temperature reaches 1.5, the seed liquid is prepared; then, the seed liquid is inoculated to the transparent plastic agricultural film at 10% inoculation amount, at 30~35°C (when the temperature of the day is too high, spray water to cool down), and use sunlight to light Cultivate for 3 days, and irradiate with 60W / 25L incandescent lamp on cloudy days and nights. When the bacterial liquid turns deep red, the cultivation is completed.

[0056] Concentration and solidification of strain PSB20: Add aluminum potassium sulfate solution with a concentration of 0.5g / L to the cultured bacterial solution of strain PSB20, let it stand overnight for flocculation, and remove the supernatant; then add solid trehalose, diatomaceous earth ( crushing fineness 120 mesh), and then air-dried at 30°C to obtai...

Embodiment 2

[0061] Cultivation of strain PSB20: take strain PSB20 and inoculate it into medium Ⅰ, and culture it under light at 30°C and 3000lx for 96h, when the OD of the culture medium is 660 When the temperature reaches 1.7, the seed liquid is prepared; then inoculate the seed liquid to transparent plastic agricultural film according to the inoculation amount of 5%, at 30~35°C (when the temperature of the day is too high, spray water to cool down), and use sunlight to illuminate Cultivate for 5 days, and irradiate with 60W / 25L incandescent lamp on cloudy days and nights. When the bacterial liquid turns deep red, the cultivation is completed.

[0062] Concentration and solidification of strain PSB20: Add aluminum potassium sulfate solution with a concentration of 0.7g / L to the cultured bacterial solution of strain PSB20, let it stand overnight for flocculation, and remove the supernatant; then add solid trehalose and zeolite powder (crushed fineness 250 mesh), and then air-dried at 40°C...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Grinding finenessaaaaaaaaaa
Finenessaaaaaaaaaa
Finenessaaaaaaaaaa
Login to view more

Abstract

The invention discloses a microbial preparation for improving an aquatic product culture environment and a preparation method thereof. A strain with optimal nitrite degradation effects is screened and separated from a mud sample in Tongan and Haicang ports of Xiamen city of the Fujian province, another strain with optimal nitrite degradation effects is screened and separated from a mud sample in the intertidal zone of Ningde city of the Fujian province, and the two strains respectively are Rhodopseudomonas palustris PSB20 and Bacillus subtilis ND04. The microbial preparation prepared from the two strains can degrade ammonia nitrogen and nitrous acid in an aquatic product culture environment, has good decomposition effects on residual bait organic matters, has a certain effect of inhibiting pathogenic vibrio bacterium growth in culture water, and has a good production application prospect. The microbial preparation has the characteristic of simple preparation processes.

Description

technical field [0001] The invention belongs to the technical field of aquaculture water quality improvement, and in particular relates to a microbial preparation applied to aquaculture environment improvement and a preparation method thereof. Background technique [0002] With the development of the aquaculture industry, ponds are aging year by year, and the water quality and bottom quality of aquaculture are seriously polluted, resulting in frequent occurrence of aquaculture diseases. Water quality improvement has become one of the main measures for healthy aquaculture. [0003] In recent years, the research on water quality improvers is also in continuous development. Traditional water quality improvers use physical and chemical principles to improve water quality by absorbing or increasing dissolved oxygen in water. The efficiency is low, and it cannot meet the requirements of water purification in the current high-density farming mode. Most of them are biological water...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C02F3/34C12R1/01C12R1/125
CPCC02F3/34C02F2101/16C02F2101/166C12N1/20C12N1/205C12R2001/125C12R2001/38
Inventor 徐长安唐旭刘源森林凌方卫东黄仕新
Owner 福建汇盛生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap