Microbial preparation for improving aquatic product culture environment and preparation method thereof
A microbial preparation and aquaculture technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of biological bactericides that have not been found yet, achieve good production and application prospects, simple preparation methods, and easy decomposition effect of effect
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preparation example Construction
[0045] And the specific operation steps of the microbial preparation preparation method are as follows:
[0046] (1) Cultivation of Rhodopseudomonas palustris strain PSB20: Take Rhodopseudomonas palustris strain PSB20 and inoculate it into medium Ⅰ, and culture it at 25-35°C and 2500-3500lx under light for 72-144h, When the OD of the culture medium 660 When the seed liquid reaches 1.5-2.2, the seed liquid is prepared; then the seed liquid is inoculated to transparent plastic agricultural film according to the inoculum amount of 5-20%, and cultivated at 30-35 °C for 3-5 days under sunlight, and the cloudy, And at night, use 60W / 25L incandescent lamp to irradiate, when the bacterial liquid turns deep red, the cultivation is completed;
[0047] (2) Concentration and solidification of Rhodopseudomonas palustris strain PSB20: add a flocculant to the cultured bacterial liquid obtained in step (1), let stand overnight to flocculate, and remove the supernatant; then add the protecti...
Embodiment 1
[0055] Cultivation of strain PSB20: take strain PSB20 and inoculate it into medium Ⅰ, and culture it under light at 27°C and 2500lx for 72h, when the OD of the culture medium is 660 When the temperature reaches 1.5, the seed liquid is prepared; then, the seed liquid is inoculated to the transparent plastic agricultural film at 10% inoculation amount, at 30~35°C (when the temperature of the day is too high, spray water to cool down), and use sunlight to light Cultivate for 3 days, and irradiate with 60W / 25L incandescent lamp on cloudy days and nights. When the bacterial liquid turns deep red, the cultivation is completed.
[0056] Concentration and solidification of strain PSB20: Add aluminum potassium sulfate solution with a concentration of 0.5g / L to the cultured bacterial solution of strain PSB20, let it stand overnight for flocculation, and remove the supernatant; then add solid trehalose, diatomaceous earth ( crushing fineness 120 mesh), and then air-dried at 30°C to obtai...
Embodiment 2
[0061] Cultivation of strain PSB20: take strain PSB20 and inoculate it into medium Ⅰ, and culture it under light at 30°C and 3000lx for 96h, when the OD of the culture medium is 660 When the temperature reaches 1.7, the seed liquid is prepared; then inoculate the seed liquid to transparent plastic agricultural film according to the inoculation amount of 5%, at 30~35°C (when the temperature of the day is too high, spray water to cool down), and use sunlight to illuminate Cultivate for 5 days, and irradiate with 60W / 25L incandescent lamp on cloudy days and nights. When the bacterial liquid turns deep red, the cultivation is completed.
[0062] Concentration and solidification of strain PSB20: Add aluminum potassium sulfate solution with a concentration of 0.7g / L to the cultured bacterial solution of strain PSB20, let it stand overnight for flocculation, and remove the supernatant; then add solid trehalose and zeolite powder (crushed fineness 250 mesh), and then air-dried at 40°C...
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