Denitrifying strain with nitrifying function, strain-containing water body improver of multiple active microorganisms and preparation method of water body improver

A technology of water conditioner and nitrifying strain, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as weak adaptability of spoiled water quality, inability to synergistically exhibit effects, and large differences in effects.

Active Publication Date: 2012-06-13
JIANGSU DAYOU BIOLOGICAL DEVAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are many micro-ecological water body improvers based on the action of microorganisms on the market, but at present, these water body improvers have low survival rate of live bacteria, different microorganisms cannot coexist well in water bodies, and have poor adaptab...

Method used

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  • Denitrifying strain with nitrifying function, strain-containing water body improver of multiple active microorganisms and preparation method of water body improver
  • Denitrifying strain with nitrifying function, strain-containing water body improver of multiple active microorganisms and preparation method of water body improver

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The screening of the highly active protease producing strain with nitrifying function of embodiment 1:

[0036] Microbial strains suitable for water quality improvement were isolated from the sediment and water bodies of aquaculture ponds, and the genus and species of the strains were effectively and accurately determined by means of morphological observation, physiological and biochemical experiments, and 16S rDNA analysis of the strains.

[0037]Weigh 2 g of the sediment sample obtained from an aquaculture pond in Jiangsu, add 98 mL of sterile saline, shake at 120 rpm on a shaker at 37°C for 20 min, absorb 1 mL of the supernatant and add nitrate as In the only nitrogen source medium, shake for 6 h, dilute the enrichment solution by 10000 times, and use a coating rod to spread it into the medium with sodium nitrate, sodium nitrite and ammonium sulfate as the only nitrogen source, and keep the temperature at 37 °C Cultivate in the incubator for 40-48 hours to obtain a s...

Embodiment 2

[0041] Embodiment 2: bacterial strain expansion culture and product collection

[0042] According to the aforementioned bacterial strain expansion culture method, the bacillus subtilis of inclined plane is according to following ( figure 1 Shown) expansion method, to obtain a certain concentration of Bacillus subtilis spore liquid, and then through means such as centrifugation and spray drying, to obtain the spore powder product.

[0043] After the above cultivation and follow-up operations, the number of spores of the final spore powder obtained can reach 1×10 11 ~2×10 12 cfu / g.

Embodiment 3

[0044] Embodiment 3: the preparation method of micro-ecological water body improver (solid)

[0045] Mix the screened Bacillus subtilis and other effective strains according to the ratio of the number of live bacteria in their respective solid bacteria powders, and the proportion of live bacteria in the solid bacteria powder of each effective strain (calculated according to the total number of live bacteria in the product) is as follows:

[0046] Bacillus subtilis 2×10 8 cfu, Bacillus licheniformis 1 x 10 8 cfu, Bacillus pumilus 1 x 10 8 cfu, Bacillus cereus 3 x 10 8 cfu, Bacillus megaterium 3 x 10 7 CFU, Rhodopseudomonas palustris 1 x 10 7 cfu, red yeast 1 x 10 7 cfu, Arthrobacter globosa 5 × 10 7 cfu, Lactobacillus plantarum 1×10 7 cfu.

[0047] After the above bacterial powders are mixed evenly according to the predetermined ratio, one or more of bran powder, glucose, corn flour, rice bran powder, zeolite powder, diatomaceous earth, and sericite powder is a...

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Abstract

The invention discloses a denitrifying strain with nitrifying function, a strain-containing water body improver of multiple active microorganisms and a preparation method of the water body improver, belonging to the technical fields of aquaculture and water body improvement. The strain disclosed by the invention is conserved in the China center for type culture collection (CCTCC), the CCTCC NO isM2011443; and the water body improver of the active microorganisms which is based on the strain comprises multiple strains such as Bacillus subtilis, Bacillus licheniformis, Bacillus pumilus, Bacillus cereus, Bacillus megaterium, Rhodopeudomonas palustris, Arthrobacter globiformis, Rhodotorula rubra and Lactobacillus plantarum. The strain is used to perform liquid fermentation and obtain fermentation liquor of which number of live bacteria is 0.5-3*10<10>cfu/mL, bacterial powder of which the number of live bacteria is 0.2-2*10<12>cfu/mL is further prepared; and the bacterial powder is used along with a diluent to prepare a liquid or powdery water body improver, wherein the effective live bacteria of the water body improver is 0.1-3*10<10>cfu/mL(or cfu/g). The water body improver has the function of reducing the contents of ammonia nitrogen, nitrites and nitrates in the culture water body; and the water body improver is suitable for different culture water bodies and has the function of improving the water quality.

Description

technical field [0001] The invention relates to a formula and a preparation method of a denitrifying bacterial strain Bacillus subtilis with nitrification function, and a variety of microorganisms including the bacterial strain, which are suitable for different aquaculture water bodies. The water body improving agent can be used in the fields of aquaculture, water quality purification, waste treatment, environmental protection and the like, and mainly belongs to the fields of aquaculture and water body improvement. Background technique [0002] In the aquaculture industry, a large amount of residual bait, excrement, etc. will accumulate at the bottom of the aquaculture water area, which will cause water body corruption, a large number of pathogenic bacteria, and lead to a large number of fish and shrimp deaths during the aquaculture process. [0003] At present, there are many micro-ecological water body improvers based on the action of microorganisms on the market, but at p...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/125
Inventor 张梁石贵阳丁重阳顾正华
Owner JIANGSU DAYOU BIOLOGICAL DEVAL
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