Method capable of improving adipose survival rate and regeneration of adipose tissue

A fat tissue and survival rate technology, applied in tissue regeneration, bone/connective tissue cells, pharmaceutical formulations, etc., can solve problems such as volume increase, destruction, and mature adipocytes are susceptible to external mechanical damage, so as to accelerate vascularization and Effects of revitalization, blood vessel formation, improvement of blood circulation and nutrient supply function

Inactive Publication Date: 2017-12-15
石家庄瑞贝尔医疗美容门诊部
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  • Abstract
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Problems solved by technology

Mature adipocytes can only increase the volume of the transplanted site and mature adipocytes are vulnerable

Method used

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Examples

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Example Embodiment

[0015] This method of improving the fat survival rate and regeneration of adipose tissue includes the following steps:

[0016] a. Extract pre-adipocytes

[0017] Under aseptic conditions, extract the postoperative adipose tissue mixture from the human adipose tissue through the washing solution (d-hank's washing solution, which is made of sodium chloride, disodium bicarbonate, potassium chloride, etc.) After the blood cells are washed, they are allowed to stand in sterile saline at 2~5°C for 40 minutes; then the centrifuge is set to 1500 rpm again, after ten minutes of centrifugation, the supernatant is discarded to obtain pre-adipocytes.

[0018] Pre-adipocytes are separated from the adipose tissue mixture after extraction from human adipose tissue. Adipose tissue has the advantages of convenient material extraction and high stem cell content. According to the average experimental data, 2X108~6X108 adipose interfills can be obtained per 30ml adipose tissue Cytoplasmic stem cells. ...

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Abstract

The invention discloses a method for improving fat survival rate and regeneration of adipose tissue, which includes extracting pre-adipocytes, transplanting fat cells, extracting nutrient components and backfilling and transplanting. The fat cells obtained by the method of the present invention can continuously differentiate into fat cells after transplantation, which solves the problems of traditional fat transplantation absorption activity and fat cell transplantation absorption or necrosis; the transplanted fat can survive in a long-term, lasting and stable manner. The material extracted by the present invention is transformed into fat cells after transplantation, which can promote the continuous growth of the original fat cells, and eventually become stable fat tissue, with a survival rate as high as 90%. Activated adipocytes transform into adipocytes, form adipose tissue at the site to be filled, promote the continued growth of original adipocytes, help form blood vessels, accelerate vascularization and resurvival of grafted fat, and release factors that promote angiogenesis, which can The rapid survival of transplanted fat into normal and viable adipose tissue can significantly improve the patient's physical beauty.

Description

technical field [0001] The invention relates to a method for the survival and regeneration of fat cell autologous transplantation. Background technique [0002] Since Neuber first reported the success of free fat transplantation in 1893, many medical and aesthetic experts have used this method to perform a large number of filling repairs on breasts, faces and other parts, but none of them have achieved the expected surgical results. Fat is mainly distributed in human subcutaneous tissue, omentum, mesentery and perirenal and other parts. Adipose tissue is composed of preadipocytes and mature adipocytes. The patina in mature adipocytes contains 90% lipid droplets, which can be quickly metabolized after transplantation. Mature adipocytes are terminally differentiated cells that have lost the ability to continue proliferating. Mature adipocytes can only increase the volume of the transplanted site and mature adipocytes are vulnerable to external mechanical damage, so that a co...

Claims

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Application Information

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IPC IPC(8): C12N5/077A61L27/38A61L27/36
CPCC12N5/0653A61L27/3616A61L27/3834A61L27/3895A61L2400/06A61L2430/04
Inventor 张继伟王红娟李雪梅王占利
Owner 石家庄瑞贝尔医疗美容门诊部
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