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Method for overcoming interspecific hybridization obstacles of water lily

A water lily and barrier technology, applied in the field of overcoming water lily interspecific hybridization barriers, can solve the problems affecting gene transfer of excellent flower color, low hybridization seed setting rate, poor affinity, etc., to achieve the effect of overcoming hybridization barriers, scientific and reasonable methods, and promoting identification

Pending Publication Date: 2020-07-10
ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, cross-subgenus hybridization between water lily species encounters difficulties, especially the poor hybrid compatibility between cold-resistant water lily and tropical water lily, resulting in low or no seed setting rate of hybridization, which seriously affects the transfer of excellent color genes of tropical water lily to cold-resistant water lily. Therefore, solving this problem is of great significance for the breeding of new varieties of water lilies with specific flower colors.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Material selection

[0023] 'Peter' (Nymphaea'Peter Slocum') and Xiaohua (Nymphaea micrantha) (both are well-known varieties, see references Huang Guozhen, Deng Huiqin, Li Zuxiu, Li Gang. Water lily. China Forestry Publishing House, Beijing, 2009),'Peter' flower color It is a light pink flower, the flower type is cup-shaped, and the small flower color is light blue-purple, the flower type is star-shaped. If the flower color of the floret is transferred to'Peter', a beautiful light blue-purple flower with multiple petals will be cultivated. In fact, after many years of hybridization practice, hybrid seeds of the'Peter' and the floret cannot be obtained because of the existence Obstacles before fertilization.

[0024] 2. Obtaining hybrids of ‘Peter’ and Xiaohua specifically includes the following steps:

[0025] Step 1. Bagging the'Peter' flower buds the day before flowering, collect a large amount of fresh'Peter' pollen on the morning of the second day of flowering, and pl...

Embodiment 2

[0039] In order to study the influence of the amount of boric acid and calcium chloride on the seed setting rate of'Peter' and floret hybrids, this example sets up 10 sets of experiments, the amount of boric acid and calcium chloride added is shown in Table 2; the hybridization method adopts the hybridization of the present invention The method, specific steps are the same as in Example 1, and the results of hybridization rate are shown in Table 2. It can be seen from Table 2 that the hybrid seed setting rate of calcium chloride added with boric acid is higher than that of the experimental group without adding boric acid and calcium chloride, indicating that the addition of boric acid and calcium chloride does help increase the hybrid seed setting rate. In addition, adding different concentrations of boric acid and calcium chloride will affect the hybrid seed setting rate of ‘Peter’×floret to varying degrees. The addition of 10 mg / L boric acid and 600 mg / L calcium chloride to th...

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Abstract

The invention discloses a method for overcoming interspecific hybridization obstacles of water lily, and belongs to the technical field of plant breeding. The method comprises the steps of drying collected female parent pollen in the shade; then collecting stigma liquid and male parent pollen of a female parent, wherein the stigma liquid is used as pollination liquid; and finally, mixing the treated female parent pollen, fresh male parent pollen and pollination liquid, and then pollinating the mixture to obtain hybrid seeds. According to the method, the problem that pollen is low in germination rate and even does not germinate on stigmas when cold-resistant water lily and tropical water lily are hybridized is solved, the treated female parent pollen and fresh male parent pollen are mixed,recognition of stigma and pollen in the water lily hybridization process is promoted, and then pollen germination and pollen tube growth are promoted.

Description

Technical field [0001] The invention belongs to the technical field of plant breeding, and specifically relates to a method for overcoming the hybridization barrier between water lilies. Background technique [0002] Water lily is the collective name of Nymphaea plants. There are about 50 native species of Nymphaea at home and abroad, and 5 native species distributed in China. In recent years, with the improvement of people's living standards and the vigorous development of leisure agriculture, there is an increasing demand for new varieties of water lilies with rich colors and long flowering periods. Therefore, the selection and breeding of new water lily varieties is particularly important. [0003] At present, hybrid breeding is still the main method for breeding new water lily varieties. However, at present, the cross-subgenus hybridization of water lily species encounters difficulties, especially the poor compatibility of cold-resistant water lily and tropical water lily, re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01N59/08A01N59/14A01P21/00
CPCA01H1/02A01N59/08A01N59/14
Inventor 孙春青戴忠良姚悦梅徐迎春山溪
Owner ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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