Preparation method of organ-like structure based on microfluidic technology high-throughput culture

A microfluidic technology and organoid technology, which is applied in the field of organoid preparation, can solve the problems that are difficult to meet clinical application and scientific research, low success rate, and reduce cell activity, so as to facilitate spatial arrangement and assembly, and benefit long-term Stable survival, guarantee the effect of material exchange

Pending Publication Date: 2022-05-27
杭州海兰时生物科技有限责任公司
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Problems solved by technology

However, due to the enzymatic hydrolysis method to digest tissues and destroy intercellular connections, and the digestion time is longer, which reduces cell activity, the s

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  • Preparation method of organ-like structure based on microfluidic technology high-throughput culture
  • Preparation method of organ-like structure based on microfluidic technology high-throughput culture
  • Preparation method of organ-like structure based on microfluidic technology high-throughput culture

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Embodiment Construction

[0033] The technical solution of the present patent will be described in further detail below in conjunction with specific embodiments.

[0034] Embodiments of the present patent are described in detail below, examples of which are illustrated in the accompanying drawings, wherein the same or similar reference numerals refer to the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present patent, but should not be construed as a limitation on the present patent.

[0035]In the description of this patent, it should be understood that the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", The orientation or positional relationship indicated by "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the c...

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Abstract

The invention belongs to the technical field of organoid preparation, and particularly relates to a preparation method of an organoid structure based on micro-fluidic technology high-throughput culture, which comprises a cell culture substrate, a micro-fluidic chip, a micro-fluidic control system, a high-throughput trace drug library modeling module and a micro-fluidic high-throughput drug screening module, the method specifically comprises the following steps: step 1, obtaining an organoid tissue sample, and cutting the organoid tissue sample into tissue blocks suitable for mechanical grinding; 2, the tissue blocks are ground through a mechanical grinding method, first-stage filtering is conducted through a first-stage filter screen with the pore diameter being 80-200 micrometers, and first-stage filtrate is formed; and step 3, carrying out second-stage filtration on the first-stage filtrate through a second-stage filter screen with the aperture of 30-60 [mu] m. The proliferation capacity of the organoid treated by the method is obviously higher than that of the organoid treated by an enzymolysis method, and the mechanical method can remarkably enrich tumor cells and remove normal cells, so that the tumor organoid with higher purity is obtained.

Description

technical field [0001] The invention relates to the technical field of organoid preparation, in particular to a preparation method of an organoid structure based on microfluidic technology for high-throughput culture. Background technique [0002] Organoids are 3D cell cultures grown in vitro using adult stem cells, which have highly similar histological characteristics to corresponding human organs, and can reproduce part of the organ's physiological functions. With the gradual deepening of related research, organoids have become a hot research topic in the scientific community. [0003] Organoid research meets the current needs of individual precision medicine design, especially in the field of organoid mechanism and related drug screening and model building, which has outstanding prospects, helping to accelerate the development of new drugs, and is a powerful tool for new drug research and development. The application of organoids can obtain the expected curative effect ...

Claims

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Application Information

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IPC IPC(8): C12N5/09C12M3/00C12M1/36C12M1/12
CPCC12N5/0693C12M23/16C12M33/00C12M25/14C12M41/00C12M45/02C12M33/14C12N2513/00
Inventor 王宇赫张丽媛马骁骅胡建鸿刘胜男
Owner 杭州海兰时生物科技有限责任公司
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