Preparation method of organ-like structure based on microfluidic technology high-throughput culture

Abstract

The invention belongs to the technical field of organoid preparation, and particularly relates to a preparation method of an organoid structure based on micro-fluidic technology high-throughput culture, which comprises a cell culture substrate, a micro-fluidic chip, a micro-fluidic control system, a high-throughput trace drug library modeling module and a micro-fluidic high-throughput drug screening module, the method specifically comprises the following steps: step 1, obtaining an organoid tissue sample, and cutting the organoid tissue sample into tissue blocks suitable for mechanical grinding; 2, the tissue blocks are ground through a mechanical grinding method, first-stage filtering is conducted through a first-stage filter screen with the pore diameter being 80-200 micrometers, and first-stage filtrate is formed; and step 3, carrying out second-stage filtration on the first-stage filtrate through a second-stage filter screen with the aperture of 30-60 [mu] m. The proliferation capacity of the organoid treated by the method is obviously higher than that of the organoid treated by an enzymolysis method, and the mechanical method can remarkably enrich tumor cells and remove normal cells, so that the tumor organoid with higher purity is obtained.

Description

technical field [0001] The invention relates to the technical field of organoid preparation, in particular to a preparation method of an organoid structure based on microfluidic technology for high-throughput culture. Background technique [0002] Organoids are 3D cell cultures grown in vitro using adult stem cells, which have highly similar histological characteristics to corresponding human organs, and can reproduce part of the organ's physiological functions. With the gradual deepening of related research, organoids have become a hot research topic in the scientific community. [0003] Organoid research meets the current needs of individual precision medicine design, especially in the field of organoid mechanism and related drug screening and model building, which has outstanding prospects, helping to accelerate the development of new drugs, and is a powerful tool for new drug research and development. The application of organoids can obtain the expected curative effect ...

AI Technical Summary

Problems solved by technology

However, due to the enzymatic hydrolysis method to digest tissues and destroy intercellular connections, and the digestion time is longer, which reduces cell activity, the success rate of sample processing is low, and the number of organoids obtained is small, which is difficult to meet the needs of clinical applications and scientific research.

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