Probiotics microbial agent for regulating livestock intestinal flora and preparation method thereof

A technology of intestinal flora and probiotics, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of the quality decline of milk, eggs and various meat products, the easy production of respiratory tract and intestinal tract in livestock and poultry. Diseases, poor quality of the breeding environment and surrounding environment, etc., to prevent intestinal diseases and respiratory diseases of livestock, improve the breeding ecological environment and surrounding environment, and improve the efficiency of feed utilization.

Inactive Publication Date: 2013-04-24
赵颖 +1
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] There have been many reports about the abuse of antibiotics and veterinary drugs in the breeding industry to prevent the disease of poultry and livestock. This not only increases the cost of breeding, but also leads to a decline in the quality of milk, eggs and various meat products, increasing the risk of unhealthy conditions, and seriously affecting the sustainable development of the breeding industry.
However, in the breeding industry, d...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0014] Preparation of bacteria:

[0015] 1. According to the conventional process of microbial fermentation engineering, the Candida utilis strains preserved on the slant are first activated with potato medium, and then expanded step by step through test tube (about 10mL) - triangular flask (about 100mL) - large triangular flask (about 1000mL) Cultivated and prepared into the first-grade seed bacterial liquid of Candida utilis; similarly, 5 kinds of bacteria such as Streptococcus faecalis were respectively activated with beef extract peptone medium, and were gradually expanded and cultivated through test tube-triangle flask-large triangle flask, and prepared into The primary seed liquid of 5 kinds of bacteria such as Streptococcus faecalis.

[0016] 2. Carry out secondary and tertiary expansion cultivation of the primary seed bacterial liquid of 5 kinds of bacteria such as Candida utilis and Streptococcus faecalis with corn flour-soybean meal-potato-sucrose culture medium.

...

Embodiment 1

[0036] 1. First, activate the strains of Candida utilis stored on the slant with potato medium (PDA), and then expand and cultivate them step by step in three stages to prepare the first-grade seed liquid of Candida utilis; similarly, Streptococcus faecalis , Bacillus subtilis, Bacillus natto, Lactobacillus plantarum and Rhodopseudomonas palustris were activated with beef extract peptone medium respectively, and after three-stage step-by-step expansion culture, 5 kinds of bacteria including Streptococcus faecalis were prepared The primary seed bacterial liquid (total 5000mL) was transferred to the secondary fermentation and expanded culture under sterile conditions.

[0037] 2. Corn flour-soybean meal-potato-sucrose medium for secondary expansion cultivation, a total of 60L, fermented for 24h at 30°C, rotating speed 100-200r / min, 0.05Mpa; sample inspection under sterile conditions, extract 10mL The fermentation broth was diluted 100 times, and the absorbance value (OD) was mea...

Embodiment 2

[0042] 1. As in Example 1, activate Candida utilis and Streptococcus faecalis and other 6 kinds of bacteria step by step, respectively, and expand the culture, then mix the equal volumes of the bacteria liquid of each bacteria, and use it as the primary fermentation seed liquid (total 5400mL) , transferred to secondary fermentation and expanded culture under sterile conditions.

[0043] 2. Corn flour-soybean meal-potato-sucrose medium for secondary expansion cultivation, a total of 50L, fermented for 24h at 30°C, rotating speed 100-200r / min, 0.05Mpa; sample inspection under sterile conditions, extract 10mL The fermentation broth was diluted 100 times, and the absorbance value (OD) was measured at a wavelength of 660nm. After the OD value reached 0.5-0.7, it was transferred to the third-stage expansion culture.

[0044] 3. The third-level expansion culture is expanded to 500L, cultivated with corn flour-soybean meal-potato-sucrose medium, and the bacterial liquid of the second-...

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Abstract

The invention discloses a probiotics microbial agent for regulating livestock intestinal flora and a preparation method thereof. The probiotics microbial agent is prepared by using candida utilis, streptococcis faecium, bacillus subtilis, bacillus natto, lactobacillus plantarum and rhodopseudomonas palustris as raw materials, performing the three-stage liquid fermentation, then uniformly mixing the enlarged cultivated fermenting bacteria with a feed wheat bran, performing the solid fermentation, drying at low temperature to obtain the probiotics agent for regulating the livestock intestinal flora structure. The agent can be used for regulating the livestock intestinal flora structure, promoting the growth of the probiotics, inhibiting the growth of the harmful bacteria, substituting the antibiotics, improving the feed utilization rate, obviously reducing the odor of the excrement of the livestock and improving the ecological environment of aquiculture and round environment, and is efficient, non-toxic and no residue. The probiotics microbial agent can be widely applied to a livestock feed additive so as to prevent the livestock intestinal disease and respiratory disease.

Description

technical field [0001] The invention relates to a probiotic microbial agent for adjusting the intestinal flora of poultry and livestock and a preparation method thereof, specifically, a probiotic for adjusting the intestinal flora structure of poultry and livestock and replacing antibiotics to prevent intestinal and respiratory diseases of poultry and livestock Bacteria and its preparation method. Background technique [0002] There have been many reports about the abuse of antibiotics and veterinary drugs in the breeding industry to prevent the disease of poultry and livestock. This not only increases the cost of breeding, but also leads to a decline in the quality of milk, eggs and various meat products, increasing the risk of unhealthy conditions, and seriously affecting the sustainable development of the breeding industry. . However, in the breeding industry, due to intensive farming, the quality of the breeding environment and the surrounding environment is poor, a...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16A23K1/16A23K1/18C12R1/72C12R1/46C12R1/125C12R1/07C12R1/25C12R1/01
Inventor 张清敏赵颖
Owner 赵颖
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