Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for rapidly and accurately identifying sturgeon sex

A sturgeon, accurate technology, applied in the field of molecular biology, can solve the problem of misjudgment and false negative of sturgeon sex samples

Active Publication Date: 2021-04-20
FUJIAN NORMAL UNIV
View PDF8 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the technical problems such as misjudgment of sturgeon sex samples and occurrence of false negatives in the prior art, the present invention provides a method for quickly and accurately determining the sex of sturgeons. For gene sequences with the same subsequence but different intron sequences, use this difference to design specific primers for PCR amplification, and use double positive results to determine male and female, and improve the accuracy and stability of sturgeon sex determination

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for rapidly and accurately identifying sturgeon sex
  • Method for rapidly and accurately identifying sturgeon sex
  • Method for rapidly and accurately identifying sturgeon sex

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Primer Design and Synthesis

[0024] The published genome sequences of sturgeon were collected, and through multiple alignment and analysis, the specific gene sequences with the same exon sequence and different intron sequences in the W chromosome and the Z chromosome were screened out. The specific gene sequence of the W chromosome is SEQID NO: 3, the specific gene sequence of Z chromosome is SEQ ID NO:4; Design specific primer F (gene sequence is SEQ ID NO:1) and primer R (gene sequence is SEQ ID NO:2) according to above-mentioned sequence, primer is by Synthesized by Sangon Bioengineering (Shanghai) Co., Ltd. The specific primer sequences are as follows:

[0025] The primer F: 5'-GTGTCATAGCAAAGGGGGTGAA-3';

[0026] The primer R: 5'-TCACAGGGCCCTACTTATAATGTCT-3'.

Embodiment 2

[0028] The invention provides a method for quickly and accurately identifying the sex of sturgeon, which specifically includes the following steps:

[0029] (1) Extract the DNA of the sturgeon sample to be detected

[0030] 16 3-year-old sturgeons of 4 species were provided by Fujian Long Hulus Sturgeon Industry Co., Ltd. The caudal fin tissue samples of 1 cm × 1 cm were taken respectively and stored in absolute ethanol. At the same time, the sex of the sturgeon was identified by dissection ( figure 1 is the gonad diagram of a female sturgeon and a male sturgeon), the anatomical identification results are shown in Table 1, the genomic DNA of the caudal fin of the sturgeon was extracted with a DNA extraction kit, diluted to 50ng / μL and stored at -20°C for later use; The samples for monitoring the sturgeon are the tail fin, dorsal fin, blood, puncture fluid, oral secretion, intestinal secretion and other swabs of the sturgeon;

[0031] (2) Using the sturgeon DNA sample as a tem...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for rapidly and accurately identifying sturgeon sex. The method includes the steps of (1) extracting DNA of a sturgeon sample to be detected; (2) carrying out PCR amplification by using a primer F and a primer R by taking a sturgeon DNA sample as a template; (3) carrying out agarose gel electrophoresis detection on an amplification product; and (4) judging the sex of sturgeon according to an electrophoresis result, the sturgeon is female sturgeon when two bands of 573bp and 123bp appear in amplification product bands, and the sturgeon is male sturgeon when only one band of 123bp appears in the amplification products. According to the method for rapidly and accurately identifying the sturgeon sex, gene sequences with same exon sequences and different intron sequences are found in a W chromosome and a Z chromosome of the sturgeon, a specific primer is designed by utilizing the difference to carry out PCR amplification, and the sex of the sturgeon is judged through a double-positive result, so that the accuracy and the stability of sex judgment of the sturgeon are improved.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, relates to biological molecular detection, in particular to a method for rapidly and accurately identifying the sex of sturgeon. Background technique [0002] Sturgeon, including the genus Acipenser and the genus Huso, is the only extant order in the cartilaginous squamous order. It is known as a "living fossil", and sturgeon roe sauce is known as "black gold". my country is the largest sturgeon farming country and the main exporter of sturgeon caviar. In 2019, the export volume of Chinese sturgeon caviar was 139.8 tons, and the export value was 32.535 million US dollars. However, sturgeons have a long growth cycle, slow sexual maturity, and no obvious secondary sexual characteristics. It is difficult to distinguish males and females from appearance in the early stages of development. Usually, males and females can only be distinguished after 3 to 4 years of breeding, and then the males...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6879C12Q1/686
CPCY02A40/81
Inventor 黄镇黄薇廖茜宋永康
Owner FUJIAN NORMAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com