Method for establishing oreochromisco aureus male pure line

A technology for tilapia and red tilapia, which is applied in the field of germplasm improvement of tilapia olivine, can solve problems such as the decline of excellent traits of varieties and increase of parental genetic diversity, and achieve the effect of maintaining excellent traits

Inactive Publication Date: 2015-12-23
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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AI Technical Summary

Problems solved by technology

[0002] Oni hybrid tilapia (the offspring of the male parent of Oria tilapia mated with the female parent of Nile tilapia) is one of the main species of tilapia cultured in my country. After nearly 20 to 30 years of breeding, especially the parent The overuse of the over-utilization increases the genetic diversity of the parents. It is difficult to improve the genetic traits accurately through the traditional selection method of phenotypic traits, which leads to the existence of male rate, stress resistance and other traits in the produced Oni hybrid tilapia fry. There are different degrees of difference and differentiation, and there is a decline trend in the excellent traits of varieties

Method used

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Examples

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Embodiment Construction

[0017] 1. Parental selection

[0018] During the breeding season in June, among the existing varieties of Oria tilapia, 2-year-old individuals are selected for breeding, and the breeding is carried out in cages. The size of 60 cages per cubic meter is 60, and each cage is stocked with 2 There are 3 females with 3 males, and the spawning situation of the females will be checked every other week (the eggs are contained in the mouth of the female fish), and the females with more than 1500 eggs will be selected and concentrated in the special pond for breeding as spare broodstock , Discard the female fish with less than 1500 grains. During the selection process, the female parents are appropriately supplemented to achieve the required number of selections. One month later, a total of 300 female Olia tilapia were picked out.

[0019] In June of the second year, 30 2-year-old red tilapia males were prepared, and 60 males with good gonad development and larger individuals were sele...

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Abstract

The invention discloses a method for establishing an oreochromisco aureus male pure line. Purified male oreochromisco aureus ZZ and normally-used female oreochromisco aureus zw are mated, generated male oreochromisco aureus Zz and generated female oreochromisco aureus Zw through mating each account for 50%, and the oreochromisco aureus are bred to sexual maturity. The female oreochromisco aureus Zw with the Z chromosome from the purified male oreochromisco aureus are mated with gynogenesis male oreochromisco aureus ZZ again, generated fry are bred to sexual maturity, and thus a large number of pure line male oreochromisco aureus ZZ individuals are generated. By means of the method of parent selecting, ripening accelerating, gynogenesis, mating selecting and backcross, the good oreochromisco aureus male pure line can be established, and the merits of oreochromisco aureus as the male breeding parent can be effectively maintained.

Description

technical field [0001] The invention belongs to the germplasm improvement technology of oligo tilapia. In particular, the method can establish a pure male line of Oria tilapia by means of parental selection, ripening, gynogenesis, mating selection, and backcrossing. Background technique [0002] Oni hybrid tilapia (the offspring of the male parent of Oria tilapia mated with the female parent of Nile tilapia) is one of the main species of tilapia cultured in my country. After nearly 20 to 30 years of breeding, especially the parent The overuse of the over-utilization increases the genetic diversity of the parents. It is difficult to improve the genetic traits accurately through the traditional selection method of phenotypic traits, which leads to the existence of male rate, stress resistance and other traits in the produced Oni hybrid tilapia fry. There are different degrees of difference and differentiation, and there is a decline trend in the excellent traits of varieties. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCA01K61/00Y02A40/81
Inventor 徐跑何杰强俊
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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