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Method for identifying fast and slow feather molecules of chickens

A technology of molecular identification and fast-slow, which is applied in biochemical equipment and methods, and microbial measurement/inspection, etc., can solve the problems of complex breeding process, long cycle, and restrictions on popularization and application of fast-slow feather mating lines, so as to save cultivation costs and shorten the production time. The effect of establishment time

Inactive Publication Date: 2010-01-06
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This makes the breeding process of fast and slow feather mating lines complicated (involving tedious processes such as pedigree incubation, feather type identification, and test crossing), the cycle is long, and the investment in line establishment is large, which limits the popularization and application of this technology. Therefore, many enterprises currently still use anus to distinguish male and female

Method used

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  • Method for identifying fast and slow feather molecules of chickens
  • Method for identifying fast and slow feather molecules of chickens
  • Method for identifying fast and slow feather molecules of chickens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Establishment of a molecular identification method for fast and slow chicken feathers.

[0034] 1. DNA extraction from chicken blood samples

[0035] First extract DNA from chicken blood samples with known fast and slow feather phenotype, add 200 μL TE buffer, then measure the DNA concentration, and dilute it to 100 ng / μL with TE buffer.

[0036] 2. Primer Design

[0037] According to the chicken Z chromosome fast and slow feather site sequence published by NCBI (i.e. the nucleotide sequence between the Z chromosome 9960Kbp-10140Kbp, see image 3 As shown, the URL for serial download is as follows: http: / / www.ncbi.nlm.nih.gov / projects / mapview / maps.cgi? TAXID=9031&CHR=Z&MAPS=model%2Crna%2Cge nes%2Ccntg-r&QUERY NUMBER=1&RID=4P8KETKN01R&BEG=9%2C960K&END=10%2C140K) and the difference between the fast and slow feather alleles (see figure 1), designed two pairs of primers by using the general primer design software primer5.0, evaluated the two pairs of prime...

Embodiment 2

[0053] Example 2: Reliability verification of chicken fast and slow feather molecular identification method

[0054] 1. Collection of verification materials

[0055] 210 blood samples of 1-day-old chicks identified from male to female were collected from Hubei Xinhua Ecological Livestock and Poultry Co., Ltd., including 96 roosters (slow feathers) and 114 hens (fast feathers). DNA was extracted as fast and slow by conventional methods. Materials for the reliability verification of the molecular identification method of male and female feathers.

[0056] 2. Identification of fast and slow feathers by molecular identification method of chicken fast and slow feathers

[0057] Carry out fast and slow feather identification by the chicken fast and slow feather molecular identification method established in embodiment 1.

[0058] 3. The reliability of the molecular identification method of fast and slow chicken feathers

[0059] The results of molecular identification of fast and...

Embodiment 3

[0060] Embodiment 3: Utilize the present invention to establish fast and slow feather self-sex matching line (application embodiment)

[0061] 1. Applied materials

[0062] Apply the method of the present invention to set up fast and slow feather self-sex matching line population for Jingshan Agriculture and Animal Husbandry Science and Technology Co., Ltd., Huangshi City, Hubei Province, select 1539 16-week-old breeders, including 220 roosters and 1319 hens, for each individual The wings were numbered and blood samples were collected to extract genomic DNA according to conventional methods.

[0063] 2. Identification of fast and slow feathers

[0064] Carry out fast and slow feather identification by the chicken fast and slow feather molecular identification method established in embodiment 1. The results are shown in Table 2.

[0065] Table 2 The method of the present invention is to the identification result of fast and slow feather of chicken

[0066] ...

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Abstract

The invention belongs to the technical field of animal breeding, in particular to a method for identifying fast feather type and slow feather type of chickens by applying double PCR. The method comprises the following steps: (1) designing two pairs of primers by utilizing a correlation sequence of a K gene locus found on a Z chromosome of a chicken according to structures of alleles of the fast and slow feather type; (2) extracting DNAs of chicken blood samples of the known fast and slow feather type to carry out a primer specificity test, and identifying the chicken to be a fast feather type or a slow feather type according to the size and the existence of an amplified DNA segment. When only one 350bp band is amplified, the chicken is judged to be a fast feather type, and when two bands of 350bp and 909bp are amplified, the chicken is judged to be a slow feather type. The invention also discloses a reliable checking method of the identifying method and applies the checking method to a breeding process of a self identification male and female matched system of the fast and slow feathers. The invention can shorten the breeding time of the self identification male and female matched system of the fast and slow feathers and save the breeding cost.

Description

technical field [0001] The invention belongs to the technical field of animal breeding, and in particular relates to a molecular identification method for fast and slow feathers of chickens. Background technique [0002] Sex identification is an important technology in modern chicken production. In the production of commercial laying hens, male and female chicks are eliminated through sex identification when the chicks are out of the shell, so as to save the space and cost of raising male chicks and improve the survival rate of hens; During production, separate male and female breeding through male and female identification, so that different feed and feeding management conditions can be provided according to different growth and development speeds and nutritional requirements of male and female; The farm provides single-sex breeder chicks to protect the breeding results. At present, the commonly used male and female identification methods include two types: anal turning id...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 龚炎长彭秀丽李世军俸艳萍陈忠
Owner HUAZHONG AGRI UNIV
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