Method for identifying fast and slow feather molecules of chickens

A molecular identification, fast and slow technology, used in biochemical equipment and methods, determination/inspection of microorganisms, etc.

Inactive Publication Date: 2011-08-03
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This makes the breeding process of fast and slow feather mating lines complicated (involving tedious processes such as pedigree incubation, feather type identification, and test crossing), the cycle is long, and the investment in line establishment is large, which limits the popularization and application of this technology. Therefore, many enterprises currently still use anus to distinguish male and female

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  • Method for identifying fast and slow feather molecules of chickens
  • Method for identifying fast and slow feather molecules of chickens
  • Method for identifying fast and slow feather molecules of chickens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 2. Primer Design

[0037] According to the chicken Z chromosome fast and slow feather site sequence published by NCBI (i.e. the nucleotide sequence between the Z chromosome 9960Kbp-10140Kbp, see image 3 As shown, the URL for serial download is as follows:

[0038] http: / / www.ncbi.nlm.nih.gov / projects / mapview / maps.cgi? TAXID=9031&CHR=Z&MAPS=model%2Crna%2Cgenes%2Ccntg-r&QUERY NUMBER=1&RID=4P8KETKN01R&BEG=9%2C960K&END=10%2C140K ) and the difference between the fast and slow feather alleles (see figure 1), designed two pairs of primers by using the general primer design software primer5.0, evaluated the two pairs of primers by using oligo6.0, and selected the best sequence as the primer. The primers were synthesized by Shanghai Sangon Bioengineering Technology Service Co., Ltd.

[0039] Wherein: the primer sequence that is used for double PCR amplification is as follows:

[0040] (1) The nucleotide sequence of the quality control internal standard primer that can be ...

Embodiment 2

[0057] 2. Identification of fast and slow feathers by molecular identification method of chicken fast and slow feathers

[0058] Carry out fast and slow feather identification by the chicken fast and slow feather molecular identification method established in embodiment 1.

[0059] 3. The reliability of the molecular identification method of fast and slow chicken feathers

[0060] The results of molecular identification of fast and slow feathers of chickens (for some results, see figure 2 ), of which 114 were identified as fast feathers and 96 slow feathers. Compared with the phenotypic identification results of fast and slow feathers of chickens, the accuracy rate was 100%.

[0061] Embodiment 3: Utilize the present invention to establish fast and slow feather self-sex matching line (application embodiment)

[0062] 1. Applied materials

[0063] Apply the method of the present invention to set up fast and slow feather self-sex matching line population for Jingshan Agricul...

Embodiment 3

[0064] 2. Identification of fast and slow feathers

[0065] Carry out fast and slow feather identification by the chicken fast and slow feather molecular identification method established in embodiment 1. The results are shown in Table 2.

[0066] Table 2 The method of the present invention is to the identification result of fast and slow feather of chicken

[0067] Unit: only

[0068]

[0069] 3. Establishment of maintenance system

[0070] Mate each slow-feather rooster with a fast-feather hen, and observe the feather growth rate of the offspring (observe at least 7 chicks for each cock). The offspring are all slow-feather, indicating that the cock is homozygous for KK; The rooster is heterozygous and should be eliminated. The test cross found that 21 of the slow-feather cocks were homozygous, and the others were heterozygous. A homozygous slow-feather cock is mated with a slow-feather hen, and a fast-feather cock is mated with a fast-feather hen to maintain two pare...

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Abstract

The invention belongs to the technical field of animal breeding, in particular to a method for identifying fast feather type and slow feather type of chickens by applying double PCR. The method comprises the following steps: (1) designing two pairs of primers by utilizing a correlation sequence of a K gene locus found on a Z chromosome of a chicken according to structures of alleles of the fast and slow feather type; (2) extracting DNAs of chicken blood samples of the known fast and slow feather type to carry out a primer specificity test, and identifying the chicken to be a fast feather typeor a slow feather type according to the size and the existence of an amplified DNA segment. When only one 350bp band is amplified, the chicken is judged to be a fast feather type, and when two bands of 350bp and 909bp are amplified, the chicken is judged to be a slow feather type. The invention also discloses a reliable checking method of the identifying method and applies the checking method to a breeding process of a self identification male and female matched system of the fast and slow feathers. The invention can shorten the breeding time of the self identification male and female matchedsystem of the fast and slow feathers and save the breeding cost.

Description

technical field [0001] The invention belongs to the technical field of animal breeding, and in particular relates to a molecular identification method for fast and slow feathers of chickens. Background technique [0002] Sex identification is an important technology in modern chicken production. In the production of commercial laying hens, male and female chicks are eliminated through sex identification when the chicks are out of the shell, so as to save the space and cost of raising male chicks and improve the survival rate of hens; During production, separate male and female breeding through male and female identification, so that different feed and feeding management conditions can be provided according to different growth and development speeds and nutritional requirements of male and female; The farm provides single-sex breeder chicks to protect the breeding results. At present, the commonly used male and female identification methods include two types: anal turning id...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 龚炎长彭秀丽李世军俸艳萍陈忠
Owner HUAZHONG AGRI UNIV
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