Primers, kit and detection method for detecting genders of cotuenix coturnix

A detection method and kit technology, applied in the field of molecular biology, can solve the problems of undisclosed PCR primer sequences, time-consuming and labor-intensive, and environmental pollution.
CN106435008AInactive Publication Date: 2017-02-22HENAN UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HENAN UNIV OF SCI & TECH
Publication Date
2017-02-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses primers, a kit and a detection method for detecting the genders of cotuenix coturnix, and belongs to the technical field of molecular biology. According to the invention, specific primers are designed by using length difference of CHD1 (Chromodomain-Helicase-DNA-binding protein 1) genes of the cotuenix coturnix on Z chromosome and W chromosome; genome DNAs of the cotuenix coturnix are taken as templates for performing PCR (Polymerase Chain Reaction) amplification and dissolution curve analysis; the karyotype of female cotuenix coturnix is ZW type, a PCR product contains two fragments with different sizes, and the sizes of strips are 470bp and 638bp respectively, so that the dissolution curve has two peaks; the karyotype of male cotuenix coturnix is ZZ type, a PCR product only contains one fragment, and the size of the strip is 638bp, so that the dissolution curve has one peak; the male cotuenix coturnix and the female cotuenix coturnix can be clearly distinguished by using the difference of the dissolution curve. The detection method for the genders of the cotuenix coturnix, disclosed by the invention, has the characteristics of quickness, accuracy, economy and practicality, and is suitable for high-throughput operation.
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Description

technical field

[0001] The invention relates to a primer for detecting quail sex, and also relates to a kit containing the primer and a method for detecting quail sex, belonging to the technical field of molecular biology. Background technique

[0002] Quail (Cotuenix coturnix), also known as Japanese quail, is a high-yield and high-efficiency special bird. Quail sex is closely related to its productivity. In meat quail, male quail grows faster, and the economic benefit is much higher than that of female quail; while in egg quail, the opposite is true. At present, although the self-sex matching system has been developed by livestock and poultry workers for sex identification of quails. According to the principle of sex-linked inheritance, the male and female of the offspring can be identified according to the color of the fluff at the time of hatching, but the sex of the parents remains the same. It needs to be identified by the traditional anus turning method. However, i...

Claims

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