Screening method, kit and applications of cynoglossus semilaevis gunther sex conversion genetic control site

Abstract

The present invention provides a screening method, a kit and applications of a cynoglossus semilaevis gunther sex conversion genetic control site. The screening method comprises: carrying out full-genome molecular marking on genetic female cynoglossus semilaevis gunther to obtain a plurality of SNP loci; and carrying out full-genome association analysis on the plurality of the SNP loci by adopting whether the transgenic female fish has sex conversion as the trait to obtain the cynoglossus semilaevis gunther sex conversion genetic control site. According to the present invention, from the perspective of the genetic control for controlling the sex conversion of the female fish, the full-genome analysis is performed by using the correlation between the unique SNP loci of the transgenic female fish and the sex conversion so as to find the molecular switch for controlling the sex conversion of the genetic female fish, wherein the molecular switch is the 6676874 site nucleotide on the Z chromosome; and by using the site to control the copulation method of the male cynoglossus semilaevis gunther and the female cynoglossus semilaevis gunther, the proportion of the female cynoglossus semilaevis gunther being not subjected to sex conversion in the artificial breeding cynoglossus semilaevis gunther progeny is increased so as to improve the breeding production benefits.

Description

technical field [0001] The invention relates to the field of aquaculture, in particular to a screening method, kit and application of sex reversal genetic control sites in tongue sole semismoothus. Background technique [0002] Cynoglossus semilaevis (Cynoglossus semilaevis) is a large warm-temperate coastal bottom fish that inhabits the coastal waters of China all the year round. The suitable water temperature is 14~24℃, and the suitable salt range is 14~33‰. Semi-smooth tongue sole has less natural resources, delicious taste and rich nutrition. Semi-smooth tongue sole grows fast and is large in size. After years of efforts by scientific and technological personnel, the artificial breeding technology of semi-smooth tongue sole has been very mature and applied in production. [0003] The typical biological characteristic of the semi-slick tongue is that the adult female fish and the male fish are very different, and the body size and weight of the female fish are about 2 t...

AI Technical Summary

Problems solved by technology

[0006] The main purpose of the present invention is to provide a screening method, kit and application of sex reversal genetic control loci in half-smooth tongue sole, so as to solve the problem in the prior art that the proportion of female fish in the breeding population is reduced and the production efficiency is affected

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