Sex chromosome specific molecular marker of Oreochromis aureus and genetic sex identification and monosexual fish production method based on the same

Abstract

The invention relates to a sex chromosome specific molecular marker of Oreochromis aureus and a genetic sex identification and monosexual fish production method based on the same. According to the invention, specific molecular markers of the Z chromosome and the W chromosome of the Oreochromis aureus are simultaneously screened for the first time; the Oreochromis aureus genetic sex identificationmethod constructed based on the molecular marker is accurate, stable and efficient and can be universally applied to different populations of the Oreochromis aureus to distinguish normal female fish ZW, superfemale fish WW, transformed female fish ZZ, transformed male fish ZW, and transformed male fish WW economically, quickly and accurately to obtain lots of superfemale fish WW. The method has the important significance in studying the sex determination and differentiation mechanisms of Oreochromis aureus and realizing sex control of Oreochromis aureus. Therefore, the large-scale production of the all-male fry is realized; the breeding yield is substantially increased; the breeding cost is lowered; and the economic benefits are increased.

Description

technical field [0001] The invention belongs to the field of molecular breeding, and relates to a sex chromosome-specific molecular marker of aurea tilapia and a genetic sex identification and a parthenic fish production method based on the molecular marker. Background technique [0002] Oria tilapia belongs to Perciformes (Perciformes), Cichlidae (Cichlidae), mouth hatching non-crucian carp (Oreochromis), body color is blue, so it is also called blue tilapia, native to West Africa, The lower reaches of the Nile River and Israel and other places have the characteristics of strong adaptability, high fecundity, high salt and low oxygen tolerance, strong disease resistance, delicious meat, and rich nutrition. Freshwater aquaculture objects. China is the largest producer and exporter of tilapia. [0003] Because tilapia growth has significant sexual dimorphism, male fish grows 40% to 50% faster than female fish, so it is more inclined to produce all-male tilapia in production....

AI Technical Summary

Problems solved by technology

However, the above method relies on the sex-specific molecular markers of Oria tilapia and Nile tilapia at the same time. Otherwise, the parental genotype can only be determined through test crossing, which is time-consuming and labor-intensive, which is not conducive to the application of this method.

[0007] Although there has been a patent report on sex-linked molecular markers of Olia tilapia (patent application CN101921865A), it is only limited to W chromosome-specific molecular markers, there is no Z chromosome-specific molecular markers, and it is only applicable to specific groups and does not have universal applicability

Images