Sex-specific molecular markers and their genetic sex identification method

Abstract

The invention provides a striped beakfish sex specific molecular marker and a genetic sex identification method thereof. The method comprises: screening and cloning of a male specific amplified fragment length polymorphism (AFLP) marker; performing DNA sequence analysis on sex specificity; and constructing a genetic sex polymerase chain reaction (PCR) identification method. In the invention, 4 primer combinations selected from 144 pairs of primer combinations can produce 4 male specific AFLP markers; the AFLP markers are cloned and sequenced to screen out a sequence characterized amplified regions (SCAR) marker molecular marker; and thus, the genetic sex identification method for striped beakfish is established. In the invention, the AFLP method is used for the first time to screen out the sex specific molecular marker for the striped beakfish and convert the sex specific molecular marker for the striped beakfish into the SCAR marker so as to establish the genetic sex identification method for the striped beakfish. The striped beakfish genetic sex identification method has the advantages of accuracy, sensitivity, reliability and the like, has high scientific value for the study onfish sex and has a great significance and high application value for the sex control and selective breeding of fish.

Description

technical field [0001] The invention belongs to fish molecular markers and genetic sex identification technology in the technical field of aquatic organisms, and in particular relates to a sex-specific molecular marker and a method for genetic sex identification thereof. Background technique [0002] my country is rich in fish resources, many of which have gender differences in growth rate, such as tilapia, carp, flounder, tongue sole and other fish. Therefore, research on these fish sex-related genes and sex-specific molecular markers has high theoretical significance. Rock bream belongs to the order Perciformes, the family Oplegnathidae, and the genus Oplegnathus. It is naturally distributed along the coasts of the Pacific Ocean and the Indian Ocean. It is produced in the Yellow Sea, the East China Sea, and the Taiwan Strait in China. It is a warm-temperate species. Ocean pelagic fishes. It grows fast, has delicious meat, strong disease resistance, bright color, and has ...

AI Technical Summary

Problems solved by technology

[0004] Aiming at the defects in the prior art that it is difficult to identify juvenile stonefish, the present invention provides a sex-specific molecular marker for stonefish and a genetic sex identification method thereof. The present invention screens out the sex-specific molecular marker for stonefish and uses it Based on this specific molecular marker, a genetic sex identification method was established, which provided theoretical and technical guidance for the directional breeding of stonefish

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