86 results about "Cysteine Hydrochloride" patented technology

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to application of bifidobacterium longum subsp. Infantis Y46 in the aspects of enhancing cell defense and repair and regulating metabolic homeostasis of a host. The strain is isolated from infant feces. Animal experiments prove that living cell thalli of the bifidobacterium longum subsp. Infantis Y46, which are obtained by culturing an MRS liquid culture medium added with L-cysteine hydrochloride (0.05%, v / v), can significantly enhance the oxidative stress resistance of an organism and reduce the level of active oxygen in the organism compared with a control bacterium bifidobacterium longum subsp. Infantis 15697. Transcriptomics analysis shows that the bifidobacterium longum subsp. Infantis Y46 can enhance self-protection of cells and improve physiological imbalance caused by external pressure by regulating related signal channels of an organism. The action mechanism is closely related to signaling pathways related to cell defense, metabolic homeostasis and barrier functions. The invention provides a new strain resource and an application scheme for developing functional food or medicinal preparations for enhancing the health defense function of a human body.

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to application of bifidobacterium longum subsp. Infantis Y46 in the anti-aging aspect. The bifidobacterium longum subsp. Infantis Y46 is obtained from excrement of infants. Animal experiments prove that living cell thalli (Y46) and thermally inactivated cell thalli (HKY46) obtained by culturing the bifidobacterium longum subsp. Infantis Y46 by using an MRS liquid culture medium containing L-cysteine hydrochloride (0.05%, v / v) can significantly reduce accumulation of lipofuscin in a host, and prolong the healthy life and life of the host. Therefore, the bifidobacterium longum subsp. Infantis Y46 is a promising probiotic and a promising metagen with an anti-aging function.

A pharmaceutical composition comprising sitagliptin or a pharmaceutically acceptable salt thereof, wherein the composition comprises less than 5 ppm of 7-Nitroso-3-(trifluoromethyl)- 5,6,7,8-tetrahydr

The invention provides a method for preparing a protease beverage by fermenting cyperus esculentus powder and a product thereof. Comprising the steps of directional pretreatment, fermentation medium preparation, segmented fermentation and optional blending, specifically, directional pretreatment adopts low-temperature extrusion at the temperature of 60-70 DEG C and ultrasonic treatment at the power of 200-300 W for cooperative treatment, and the fiber structure and antinutritional factors of soybean meal are effectively broken; in the fermentation stage, lactobacillus plantarum, saccharomyces cerevisiae and bacillus coagulans composite flora are inoculated, anaerobic fermentation is performed at the temperature of 35-37 DEG C, the pH value of the system is reduced to 4.0-4.5, and then L-cysteine hydrochloride-vitamin B synergist is added for secondary fermentation in a micro-aerobic environment at the temperature of 38-40 DEG C. The fermentation process provided by the invention is simple, the treatment is convenient, the used equipment is conventional equipment in the food industry, and the method is suitable for large-scale industrial production. Meanwhile, in the prepared fermentation liquor, the protease activity is larger than or equal to 320 U / mL, the total number of viable bacteria is larger than or equal to 1.2 * 10 CFU / mL, the optimum pH of the protease is 3.8-4.8, the optimum temperature is 37-39 DEG C, the protease is highly matched with the human gastrointestinal tract environment, and the method has wide industrialization prospects.

The invention relates to the technical field of food, in particular to a preparation method of low-sensitization hydrolyzed whey protein nanoscale microcapsule powder, which comprises the following steps: S1, preparing a whey protein solution, performing microfiltration, and treating with cysteine hydrochloride; s2, trypsin hydrolysis; s3, inactivating and cooling; s4, adding chymotrypsin, and synchronously applying ultrasonic waves; s5, bromelain is added, ultrafiltration is performed, and peptide fragments are collected; s6, preparing a capsule wall solution; s7, adopting a micro-fluidic chip to form a nano emulsion; s8, the mixture is added into a CaCl2 solution to be stirred, multi-layer self-assembly is conducted, and micro-capsule particles are obtained; and S9, carrying out ultrahigh-pressure micro-jet homogenization, vacuum freeze drying and low-temperature plasma assisted grinding on the micro-capsule particles to obtain the nano-scale micro-capsule powder. The stability of active ingredients is maintained through multi-enzyme cooperation and ultrasonic-assisted efficient elimination of allergens, multi-layer nano-embedding under microfluidic control and low-temperature plasma grinding, and the limitations of low single enzymolysis efficiency, insufficient embedding rate and thermal denaturation risk in the prior art are overcome.

The invention discloses a micromolecular bioluminescent probe capable of being used for monitoring in vivo / in vitro dipeptidyl peptidase IV in real time and a preparation method and application thereof.The probe is marked as DPP4-PD, during preparation, N-[t-butyloxycarbonyl] glycyl-L-proline and 6-amino-2-benzothiazole formonitrile serve as starting materials and then react with L-cysteine hydrochloric acid, and finally Boc is removed. The synthesis raw materials are easy to obtain, operation is simple, and popularization is easy. The micromolecular bioluminescence probe provided by the invention can specifically respond to DPP4 enzyme in vivo / in vitro in real time; and the probe has the remarkable advantages of high selectivity, high sensitivity, high stability, high biological safety, high signal-to-noise ratio (S / N) and the like during imaging, and has wide application prospects in the fields of pathological research, efficacy evaluation and new drug research and development.

The invention discloses lactobacillus plantarum with high antioxidant activity, and belongs to the field of probiotic preparations and biotechnology thereof. Viable bacteria and dead bacteria of the lactobacillus plantarum LCC-605 provided by the invention have extremely strong antioxidant capacity, and the capacities of removing DPPH (1, 1-diphenyl-2-picrylhydrazyl), ABTS < + > and hydroxyl free radicals respectively reach 94.7%, 99.8% and 92.5%; the superoxide anion clearance rate reaches up to 27.9%; the highest reduction capacity reaches the equivalent weight of 547.4 mu mol / L L-cysteine hydrochloride; and the iron reducing power reaches the highest OD value of 0.8592. Therefore, the lactobacillus plantarum LCC-605 can be developed as a leavening agent, is applied to fermentation of animal milk and plant milk, and develops a fermented product with a potential anti-oxidation effect. Meanwhile, the strain can also be used as a natural antioxidant, and is widely applied to preparation of food, cosmetics, health care products, medical products and animal feed in the aspect of oxidation resistance.

The invention provides a method for detecting enzyme activity of papain in a hypersensitive protein complex enzyme, which comprises the following steps: mixing the hypersensitive protein complex enzyme with an L-cysteine hydrochloride buffer solution to obtain a sample solution to be detected; adding the to-be-tested sample solution into a substrate solution containing casein, and testing a change curve of a voltage value of the to-be-tested sample solution along with time under the condition that the casein can be decomposed by papain; and determining the enzyme activity of the papain in the hypersensitive protein compound enzyme according to the change curve of the voltage value of the to-be-detected sample solution along with time. The method can solve the problem that the enzyme activity of papain in the hypersensitive protein complex enzyme cannot be accurately detected in the prior art.

The invention provides a butyric acid probiotic culture medium which comprises the following components: 6-12 g / L of peptone, 6-12 g / L of beef extract, 1-5 g / L of yeast extract, 4-8 g / L of glucose, 4-8 g / L of sodium chloride, 0.5-2 g / L of soluble starch, 1-5 g / L of sodium acetate, 0.1-1.0 g / L of L-cysteine hydrochloride, 4-8 g / L of xylan, 1-3 g / L of dipotassium phosphate, 0.1-1.0 g / L of magnesium sulfate heptahydrate and 0.01-0.05 g / L of ferrous sulfate. The invention specifically provides a method for culturing a lactobacillus plantarum and clostridium tyrobutyricum high-yield butyric acid collaborative system by using the culture medium, and the method not only improves the butyric acid yield, but also reduces the production cost of butyric acid biotransformation, and has remarkable economic benefits.

The invention discloses a large-scale preparation method of cono peptide. The large-scale preparation method of cono peptide of the invention comprises the preparation of linear peptide amino resin, cracking, linear peptide folding, crude peptide purification, using amino resin with a substitution degree of 0.4-0.8 mmol / g, sequentially coupling amino acid synthesis protected twenty-two peptide amino resin through solid phase synthesis, cracking; oxidizing one-step folding to form three pairs of disulfide bonds through cysteine ​​hydrochloride / DMSO buffer solution oxidation system, simple operation, low cost, suitable for industrial mass production; crude peptide is prepared and purified by HPLC to obtain cono peptide with a purity greater than 98%.

The invention discloses a bovine methane emission reduction method for intragastric delivery of targeted edition methanosarcina, and belongs to the technical field of biology. The method comprises the following steps: S1, preparing a conversion mixed solution: 50 mM of a PIPES buffer solution, 0.35 M of sucrose, 0.6 M of sodium chloride, 1 mM of magnesium chloride, 0.00001% of resazurin, 0.05% of cysteine hydrochloride, 1 mM of dithiothreitol, 40% of PEG4000 and 6 ml of PLL-PEG-DNA nanoparticles; s2, delivering the converted mixed solution into a ruminant rumen environment through intragastric administration; under the action of a conversion mixed solution taking polyethylene glycol 4000 as a main component, the gene editing system is delivered into methanosarcina, so that methanosarcina is modified, the number of methanosarcina in a bovine rumen is reduced, and the emission of bovine methane gas is further reduced.

The application relates to the field of biotechnology, and in particular to a wheat allergen specific IgE detection kit and a detection method thereof. The kit comprises magnetic particles, enzyme-labeled anti-human IgE antibodies and biotin-labeled wheat allergen. The wheat allergen is obtained by treating a wheat protein extract with heat treatment or a pretreatment agent. The pretreatment agent comprises one or more of sodium azide, L-cysteine and L-cysteine hydrochloride. The wheat allergen is biotin-labeled after specific pretreatment. The chemiluminescence immunoassay method uses streptavidin to prepare magnetic microspheres and horseradish peroxidase-labeled anti-human IgE antibodies. The method is fully automatic, ensures the accuracy of the detection result, and improves the test flux, the specificity and the sensitivity of the detection result.

The invention discloses a culture medium for colonization of intestinal flora and intestinal gas of people with hyperuricemia. The culture medium contains yeast extract, ethanol, adenine, tryptone, mucoprotein, mycoprotein, insect protein, soybean protein, fructose, glucose, inulin, resistant dextrin, dietary fiber, oxalic acid, saturated fatty acid, sialic acid, peanut oil, potassium chloride, sodium chloride, monopotassium phosphate, sodium sulfate, bile salt, cysteine hydrochloride and a plant extract mixture. Vitamin mixed liquid and the like. By means of the bionic colon reactor, by referring to real in-vivo parameters of people suffering from hyperuricemia, the real intestinal environment of people suffering from hyperuricemia in China is well simulated in vitro, the intestinal flora ecology and intestinal gas distribution are reproduced, and a normal intestinal microorganism and intestinal gas database of people suffering from hyperuricemia can be constructed. The method is beneficial to accurate diagnosis of intestinal microecology of people with hyperuricemia, individualized treatment of antisense booking and disease mechanisms, better maintenance of intestinal health and formulation of preventive measures.

The present disclosure relates to a method for producing L-cysteine hydrochloride hydrate crystals having a controlled particle size, and L-cysteine hydrochloride hydrate crystals having a controlled particle size produced by the production method.

The application belongs to the technical field of biological medicine, and particularly relates to a chicken synovial mycoplasma culture medium capable of improving strain titer and a preparation method thereof. The chicken synovial mycoplasma culture medium comprises a basic culture medium and auxiliary components; the basic culture medium comprises HEPES, milk protein hydrolysate, MEM, beef heart infusion powder, coenzyme I, 1% phenol red, magnesium sulfate heptahydrate, L-cysteine hydrochloride, lacto-N-tetraose, disodium chlormethine and water; and the auxiliary components comprise pig serum and penicillin. The chicken synovial mycoplasma culture medium obtained by the preparation method shortens the passage time of chicken synovial mycoplasma and improves the strain titer. The inactivated vaccine prepared from the chicken synovial mycoplasma cultured by the culture medium has good safety and immunogenicity, can effectively prevent chicken synovial mycoplasma infection, and has a good application prospect.

The application discloses a method for disinfecting and inducing regeneration of a single excellent plant of Malus baccata, and comprises the following steps: S1, disinfecting the terminal bud or / and axillary bud of the Malus baccata; S2, inoculating the terminal bud or / and axillary bud into a modified MS culture medium, and culturing under preset conditions to obtain uncontaminated terminal bud or / and axillary bud; S3, inoculating the uncontaminated terminal bud or / and axillary bud into an induction culture medium, and culturing under preset induction conditions to obtain a cluster bud, wherein the components of the induction culture medium comprise a modified MS as a base, and 6-BA, IPA, prochloraz, cysteine hydrochloride and L-arginine are added; and S4, dividing the cluster bud into single plants, inoculating the single plants into a subculture medium, and culturing under preset culture conditions to obtain Malus baccata single seedlings. Compared with the prior art, the method selects a disinfection method with a high survival rate and a modified MS culture medium with obvious plant life characteristics by taking the terminal bud or / and axillary bud of the Malus baccata as explant material, so that more uncontaminated Malus baccata terminal buds or / and axillary buds which grow well can be obtained, and the tissue culture can be carried out for a long time, thereby providing more materials for the tissue culture of the Malus baccata.

The application discloses a cassava tuber postharvest browning inhibition and fresh storage quality preservation treatment method, which comprises a three-step synergistic process of polyphenol oxidase targeted inhibition pretreatment, selective air permeable composite coating treatment and biological antagonistic bacteria surface inoculation treatment. First, the tuber is immersed in a vacuum infiltration treatment of a citric acid and L-cysteine hydrochloride composite inhibition solution, then is immersed and coated with a chitosan-sodium alginate-nano silicon dioxide selective air permeable composite coating, and finally is sprayed with bacillus subtilis inoculation. The three-step treatment synergistically realizes vascular browning inhibition for 25-30 days, and prolongs the shelf life from 2-3 days to 3-4 weeks.

The application discloses a compound amino acid injection (15-HBC) composition and a preparation method thereof, and belongs to the technical field of medicines. The compound amino acid injection (15-HBC) composition provided by the application comprises the following components in an amount of 250mL of the composition: isoleucine 1.532-2.298g, leucine 2.756-4.134g, lysine acetate 1.160-1.740g, methionine 0.500-0.750g, phenylalanine 0.640-0.960g, threonine 0.400-0.600g, tryptophan 0.180-0.270g, valine 1.772-2.658g, alanine 0.800-1.200g, arginine 1.160-1.740g, histidine 0.320-0.480g, proline 1.260-1.890g, serine 0.660-0.990g, glycine 0.660-0.990g, cysteine hydrochloride 0.040-0.060g, and appropriate amount of glacial acetic acid for pH adjustment, and the rest is injection water. The application also provides a preparation method of the compound amino acid injection (15-HBC) without sulfite antioxidant. Meanwhile, the activated carbon adsorption process is removed in the preparation, the control of raw material packages, production processes and the like is strengthened to control bacterial endotoxins, the production process is simplified, and the product quality is ensured.

The invention belongs to the technical field of pharmaceutical analysis, and discloses a method for simultaneously detecting sodium hydrogen sulfite and cysteine hydrochloride and application. Taking the concentrated solution for injection of edaravone dextroborneol, and detecting the content of an oxidizing agent by adopting high performance liquid chromatography; the oxidizing agent is sodium hydrogen sulfite and cysteine hydrochloride; a mobile phase of the high performance liquid chromatography is a 0.035% sodium heptanesulfonate solution and methanol in a volume ratio of (18-22): (78-82). According to the method, a normal-phase-mode universal chromatographic column is selected, and a proper amount of ion pair reagent is added into a reversed-phase chromatographic mobile phase, so that the retention of a sample can be enhanced, and the peak pattern can be improved. The method has remarkable advantages in specificity, linear range and repeatability, has the advantages of high specificity, high precision, high accuracy and the like, and provides an important method detection reference basis for antioxidant quality control of a concentrated solution product for edaravone dextroborneol injection.

The invention provides a pharmaceutical preparation of a cefminox sodium compound and a preparation method thereof, belonging to the technical field of drug synthesis. The preparation method comprises the following steps: using 7-MAC as a raw material, sequentially performing an acylation reaction and a decarboxylation protecting group reaction to obtain 7β-bromoacetamide-7α-methoxy-3-(1-methyl-1H-5-tetrazolyl)thiomethyl-3-cephem-4-carboxylic acid; mixing 460g of 7β-bromoacetamide-7α-methoxy-3-(1-methyl-1H-5-tetrazolyl)thiomethyl-3-cephem-4-carboxylic acid, 235g of D-cysteine ​​hydrochloride and 1.5L of water, performing ultrasonic oscillation, adding 4-10g of anthocyanin and 4-8g of polyvinyl alcohol, wherein the frequency of the ultrasonic oscillation decreases gradually within 80KHZ-45KHZ and the duration is 2h, to obtain a reaction solution; and purifying the reaction solution through a Diaion HP-20 column, concentrating the reaction solution under reduced pressure to dryness, cooling the reaction solution, and precipitating a solid to obtain the cefminox sodium compound. The cefminox sodium crystals prepared by the present invention have strong adaptability to the storage environment, the crystal particle size distribution will not easily change in a short period of time, the crystal particle size distribution has good stability, and the quality is stable and guaranteed.

The invention relates to a method for preparing snails and snail meat flavor substances based on thiamine degradation, and belongs to the technical field of food processing. The preparation method comprises the following steps: firstly, reacting thiamine or thiamine salt with a food additive 1 to obtain a reaction solution A; reacting cysteine or cysteine hydrochloride with a food additive 2 to obtain a reaction solution B; the reaction liquid A and the reaction liquid B contain important intermediates for synthesizing spirogadine; then mixing the reaction liquid A and the reaction liquid B, properly adding 2-methyltetrahydrofuran-3-ketone in the reaction process, regulating the pH value through phosphoric acid, reacting an intermediate to generate an important precursor for preparing spirogadine, and in the presence of phosphate radicals, carrying out cyclization reaction on the 2-methyltetrahydrofuran-3-ketone and the precursor to generate spirogadine, so as to obtain the spirogadine. The method effectively increases the content of the spirogadine prepared by degrading thiamine, reduces the industrial use cost, is simple to operate, is suitable for large-scale preparation, and has wide application prospects.

The invention discloses a vitamin C injection and a preparation method thereof. The preparation method of the vitamin C injection comprises the following steps: taking water for injection, which accounts for 65-80% of the total volume of the prepared solution, and introducing carbon dioxide until the water is saturated, so as to obtain a saturated solution of carbon dioxide; the preparation method comprises the following steps: adding sodium pyrosulfite, edetate disodium and cysteine hydrochloride into a saturated solution of carbon dioxide, and stirring to fully dissolve the sodium pyrosulfite, the edetate disodium and the cysteine hydrochloride to obtain a first liquid medicine; uniformly mixing vitamin C and sodium bicarbonate to obtain mixed powder, and adding the mixed powder into the first liquid medicine in batches to obtain second liquid medicine; adding activated carbon into the second liquid medicine, stirring and then filtering, replenishing the residual 20-35% of water for injection, and then finely filtering; and packaging and warehousing the finely filtered liquid medicine after encapsulation, sterilization, leak detection and light inspection. The vitamin C injection prepared by the invention is colorless and clear, the content of effective components is as high as 99.6%, and the impurity content is extremely low.

The invention discloses an isavuconazole sulfate freeze-dried powder for injection and a preparation method thereof, the isavuconazole sulfate freeze-dried powder comprises isavuconazole sulfate, a freeze-drying protective additive and a pH regulator, the freeze-drying protective additive is mannitol, chitosan modified polyvinyl alcohol and L-cysteine hydrochloride, and the pH regulator is sulfuric acid; the isavuconazole sulfate freeze-drying protective agent is prepared from the following components in parts by weight: 35-45 parts of isavuconazole sulfate and 7-9 parts of freeze-drying protective agent. Chitosan modified polyvinyl alcohol, L-cysteine hydrochloride and mannitol cooperate to form a multi-element auxiliary carrier, so that the water solubility and dispersity of mannitol at low temperature are improved, the light resistance, high temperature resistance and high humidity environment resistance of active ingredients are improved, the stability is enhanced, the storage safety of medicines is facilitated, and the quality of the medicines is ensured.

The application provides a diluent and a detection method for detecting Lactobacillus and Bifidobacterium in a double-color mixed embedding type crystal ball probiotic, and the diluent is a phosphate buffer. The phosphate buffer comprises potassium dihydrogen phosphate, sodium hydroxide, L-cysteine hydrochloride, Tween 80 and agar powder, and the mass ratio of the potassium dihydrogen phosphate, the sodium hydroxide, the L-cysteine hydrochloride, the Tween 80 and the agar powder in the phosphate buffer is 6.7-6.9:0.55-0.65:0.4-0.6:1:0.4-0.6. The oxygen sensitivity of the probiotic can be prevented by adding the L-cysteine hydrochloride, and the dispersibility of the embedding type crystal ball in the diluent can be improved by adding the Tween-80, so that the probiotic is effectively released to increase the number of the Lactobacillus and Bifidobacterium. According to the detection method, the Bifidobacterium and the Lactobacillus are selected according to the color of the crystal ball, and the number of the bacteria is detected respectively, the total number of the lactic acid bacteria is obtained by adding the number of the Bifidobacterium and the number of the Lactobacillus, and the test result is more scientific and accurate.

The vegetarian beef flavor flavoring material is mainly prepared from the following raw materials in parts by mass: 10-20 parts of distilled water, 30-50 parts of corn starch, 10-20 parts of monosodium glutamate, 0.5-5 parts of D xylose, 5-10 parts of soybean peptide paste, 1-5 parts of soybean peptide powder, 1-6 parts of glucose, 10-30 parts of edible salt, 0.5-3 parts of vitamin B1, 1-5 parts of L-cysteine hydrochloride, 0.1-1 part of taurine, 0.5-5.5 parts of disodium inosinate, 0.5-5.5 parts of disodium guanylate, 1-5 parts of sodium carbonate and 0.3-1 part of silicon dioxide. According to the vegetarian beef flavor flavoring material, the main raw materials for forming fragrance are prepared from natural meat-source-free raw materials through a Maillard thermal reaction, no meat source or animal fat is added, and the vegetarian beef flavor flavoring material is safe, healthy and natural, vivid and rich in fragrance; the invention further discloses a preparation method of the vegetarian beef flavor seasoning, and the method adopts a low-temperature vacuum drying production technology and is safe and harmless.

The invention discloses a carbocisteine analogue as well as a preparation method and application thereof, and belongs to the technical field of organic synthesis and biological medicine, and the preparation method comprises the following steps: adding cysteine hydrochloride and halogenated alkyl substituted acid into a container, then adding a solvent, stirring for dissolving, slowly dropwise adding alkali liquor for reaction at the reaction temperature of 0-100 DEG C for 1-24 hours, filtering, washing, and drying to obtain the carbocisteine analogue. And after the reaction is finished, adjusting the pH, separating out solids, and separating and purifying to obtain the product. A series of carbocisteine analogues are obtained through a simple preparation method, preliminary anti-inflammatory activity experiments show that the analogues can achieve higher anti-inflammatory activity than carbocisteine by down-regulating mRNA expression of TNF-alpha, IL-1beta and IL-6, and through further research, the compounds are expected to become lead compounds of anti-inflammatory drugs; the preparation method has the advantages of simple steps, mild reaction conditions, high yield and the like.

The invention discloses freshness-enhancing minced garlic and a preparation method thereof. The freshness-enhancing minced garlic comprises garlic granules, edible oil, edible salt, monosodium glutamate, potassium sorbate, sucrose fatty acid ester, sodium ascorbate, L-cysteine hydrochloride, water-soluble chitosan and sodium alginate. The preparation method comprises the following steps: immersing fresh garlic in a citric acid aqueous solution, crushing the immersed fresh garlic into garlic granules, adding sodium ascorbate, L-cysteine hydrochloride, water-soluble chitosan and sodium alginate into the garlic granules for pickling, adding mixed edible oil and sucrose fatty acid ester, finally adding edible salt, monosodium glutamate and potassium sorbate, and carrying out vacuum filling and sterilization. The method can reduce the discoloration of the garlic granules, maintain the original garlic fragrance of the garlic, improve the additional value of the garlic and prolong the shelf life.

The application provides a method for increasing the content of effective components of rose by using edible fungus fermentation, and belongs to the technical field of biological fermentation.The method comprises the following steps: mixing rose powder, corn starch, glucose, potassium dihydrogen phosphate and water, adjusting the pH value, sterilizing, and obtaining a rose culture medium; inoculating the rose culture medium with a chrysophic acid fungus liquid, adding L-cysteine hydrochloride after 20-30h of fermentation culture, continuing the fermentation culture for 5-7d, and obtaining a rose fermentation product.The method can significantly increase the content of quercetin, quercetin-3-O-rutinose and polyphenol in the fermentation product by inoculating the rose with the chrysophic acid fungus for solid-state deep fermentation, has obvious tyrosinase inhibition capacity, is simple in process, low in production cost, and high in safety.The application further provides the rose fermentation product prepared by the method, the rose fermentation product, and the application of the rose fermentation product in the preparation of health care products.

The invention provides a culture medium and application thereof in culture of genetically modified lactococcus lactis. The culture medium comprises a carbon source, amino acid, nucleotide, inorganic salt and vitamin, the carbon source comprises glucose; the amino acids comprise alanine, arginine, aspartic acid, asparagine, cysteine hydrochloride, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine, lysine hydrochloride, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine and valine; the nucleotide comprises adenine, guanine, uracil or xanthine; the inorganic salt comprises sodium chloride, potassium acetate, zinc sulfate heptahydrate, magnesium sulfate heptahydrate, monopotassium phosphate, dipotassium phosphate, cobalt chloride, manganese sulfate, calcium chloride, ferrous sulfate and copper sulfate. The method can solve the problem of unstable fermentation culture of lactococcus lactis in the prior art, and is suitable for the field of lactococcus lactis culture.

The invention provides a butyrate probiotic culture medium, comprising the following components: 6-12 g / L of peptone, 6-12 g / L of beef extract, 1-5 g / L of yeast extract, 4-8 g / L of glucose, 4-8 g / L of sodium chloride, 0.5-2 g / L of soluble starch, 1-5 g / L of sodium acetate, 0.1-1.0 g / L of L-cysteine hydrochloride, 4-8 g / L of xylan, 1-3 g / L of dipotassium hydrogen phosphate, 0.1-1.0 g / L of magnesium sulfate heptahydrate, and 0.01-0.05 g / L of ferrous sulfate. The invention also specifically provides a method for culturing a high-butyrate cooperative system of Lactobacillus plantarum and Clostridium tyrobutyricum using the culture medium. The method not only improves the butyrate yield but also reduces the production cost of butyrate bioconversion, thereby having significant economic benefits.