Acid-resistant bifidobacterium breve BB8dpH and application thereof

A technology of Bifidobacterium breve and strains, applied in the field of microorganisms, can solve the problems of bacterial cell death, poor acid tolerance of human-derived bifidobacteria, etc.

Inactive Publication Date: 2014-06-11
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When under low pH conditions, it will lead to a large number of bacterial cell death, especially the human-derived bifidobacterial acid tolerance is very poor

Method used

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  • Acid-resistant bifidobacterium breve BB8dpH and application thereof
  • Acid-resistant bifidobacterium breve BB8dpH and application thereof
  • Acid-resistant bifidobacterium breve BB8dpH and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: Screening and obtaining of bacterial strains

[0032] Shake 5 grams of fresh healthy young adult feces with 50ml of feces diluent into a homogenate, and add 1ml of feces homogenate into 9ml of fresh pH7.4BL liquid medium (containing 0.05% cysteine ​​hydrochloride) test tube , anaerobic culture at 37°C for 24h, use an inoculation loop to pick the bacterial solution and streak it on the TPY (containing 0.05% cysteine ​​hydrochloride) solid medium plate at pH 6.5, after 24h in anaerobic culture at 37°C, Pick a single colony and inoculate it in 50ml of fresh pH7.4BL liquid medium (containing 0.05% cysteine ​​hydrochloride), culture overnight at 37°C anaerobically to plateau stage, centrifuge at 7600g for 10min to obtain the bacterium, and then The cells were transferred to fresh BL liquid medium containing 0.05% cysteine ​​hydrochloride with a pH value of 3.2, and after anaerobic overnight culture at 37°C, centrifuged at 7600g for 10 minutes to obtain the cell...

Embodiment 2

[0033] Embodiment 2: Microscopic examination of bacterial strain

[0034] Pick up Bifidobacterium breve BB8dpH from the inoculation loop under sterile conditions, smear it on a glass slide, make a slide and perform Gram staining, and observe the morphology of the bacteria under a microscope.

Embodiment 3

[0035] Embodiment 3: strain fermentation

[0036] Inoculate Bifidobacterium breve BB8dpH in fresh pH7.4BL liquid medium (containing 0.05% cysteine ​​hydrochloride) with 1% inoculum amount, and culture anaerobically at 37° C. for 24 hours to obtain the Bifidobacterium breve BB8dpH strain of fermentation broth.

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Abstract

The invention provides an acid-resistant bifidobacterium breve BB8dpH and an application thereof. The nucleotide sequence of the 16SrRNA gene part of the bifidobacterium breve BB8dpH is shown in SEQ ID NO.1, and is stored with the preservation number of CGMCCNo.8370. The bifidobacterium breve BB8dpH is a strain obtained by separating from the dejecta of healthy young people and further screening under the pH 3.2 acid condition. According to the fermentation and culturing process, anaerobic culture at 37 DEG C is carried out in a BL liquid substrate (containing 0.05% cysteine hydrochloride) for 24 hours. The bifidobacterium breve BB8dpH has remarkably better acid resistance than the common strains, and the acid resistance has genetic stability; the bifidobacterium breve BB8dpH has different percent contents of cell membrane fatty acids from the common strains, the average carbon chain length of the bifidobacterium breve BB8dpH is remarkably longer than that of the common strains, and the cell membrane fluidity of the bifidobacterium breve BB8dpH is remarkably lower than that of the common strains; and the bifidobacterium breve BB8dpH is used in the production field of daily fermented food, health-care food and medicines.

Description

technical field [0001] The invention relates to microorganisms, in particular to an acid-resistant bifidobacterium breve BB8dpH and application thereof. Background technique [0002] The human gastrointestinal tract is colonized with various bacteria, which can be divided into beneficial bacteria, harmful bacteria and neutral bacteria according to their impact on human health. Beneficial bacteria are probiotics that have a beneficial effect on the human body, among which Bifidobacteria and Lactobacillus are the most representative probiotics that colonize the human gastrointestinal tract. It is beneficial to human health when probiotics occupy a dominant amount in the human gastrointestinal tract. [0003] Bifidobacterium belongs to Gram-positive bacteria, is an obligate anaerobic bacteria, no motility, does not produce spores during the growth cycle, and has no capsule and flagella. [0004] Bifidobacteria are very sensitive to changes in environmental pH. The optimum pH...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 杨虹杨旭杭晓敏罗程谭晶
Owner SHANGHAI JIAO TONG UNIV
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