Clear liquid fermentation medium for clostridium butyricum and fermentation culture method thereof

Abstract

The invention relates to a clear liquid fermentation medium for clostridium butyricum. The clear liquid fermentation medium, the pH value of which is 7-8, is made from glucose, tryptone, a yeast extract powder, ammonium sulfate, sodium bicarbonate, a maize liquid powder and the like. The clear liquid fermentation medium for clostridium butyricum contains metal salts for promoting the growth of gemma, wherein the metal salts are manganese sulfate, magnesium sulfate and ferrous sulphate. The enrichment medium of the clear liquid fermentation medium for clostridium butyricum contains yeast extract, beef extract, tryptone, glucose, soluble starch, sodium chloride, sodium acetate trihydrate, cysteine hydrochloride, methylene blue at the concentration of 0.5% and distilled water; and the pH is adjusted to 7.1. A fermentation culture method of the clear liquid fermentation medium for clostridium butyricum comprises the following steps of: glycerin tube refrigerated strain activation, heating and optimization, Erlenmeyer flask first-order seed culture, liquid fermentation and spray drying. The production of clostridium butyricum has advantages of simple operation, high amount of zymocyte, high gemma yield, simple post-treatment, less impurities in a bacterial powder sample and high amount of effective live bacteria, and saves cost at large.

Description

technical field [0001] The invention relates to a clear liquid fermentation medium of Clostridium butyricum and a fermentation cultivation method thereof, belonging to the technical field of microbial fermentation. Background technique [0002] Clostridium butyricum ( Clostridium butyricum ), that is, Clostridium butyricum, also known as butyric acid bacteria. It is a species in the genus Clostridium, and it mainly exists in natural environments such as cheese, natural yogurt, human and animal intestines and feces, certain leaves, and soil. Clostridium butyricum is a straight or curved Gram-positive anaerobic endophytic bacillus with some resistance to environmental changes, these characteristics make Clostridium butyricum a potential as an excellent probiotic. On the other hand, Clostridium butyricum is resistant to gastric acid and bile salts when it passes through the intestinal tract of animals, which ensures its performance. It is widely used in intestinal medicine, ...

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Problems solved by technology

[0005] The following patents CN1144873C, CN1184308C, US5.292 523 (1994), US4.892 731 (1990), JP63-146825 (1986), JP61-6625 (1984 ), JP06-166825 (1994), JP05-227898 (1993) relate to the fermentation and the preparation method of Clostridium butyricum, there are some shortcomings in these prior art,: 1, with regard to culture mode, use compound culture medium in a large number, Or other medium containing a large amount of insoluble components, which causes difficulty in post-processing, high cost, low content of live bacteria per unit of bacterial powder, and more impurities;