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Method for preparing active ginsenoside

A technology of ginsenoside and ginseng is applied in the field of preparation of active ginsenoside and can solve problems such as unfavorable strain survival and the like

Inactive Publication Date: 2017-07-25
武汉虹睿生物科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But the high temperature is also not conducive to the survival of the strain

Method used

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  • Method for preparing active ginsenoside
  • Method for preparing active ginsenoside
  • Method for preparing active ginsenoside

Examples

Experimental program
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Effect test

Embodiment 1

[0035] The preparation of embodiment 1 active PPD

[0036] Load the ginseng into the perforated rack inside the reaction chamber. Through the automatic temperature and humidity control device, steam is introduced into the reaction chamber, the air is removed, and heat treatment is performed directly by steam, and the steam pressure is 1.1kg / cm 2 . Keep the temperature at 121°C for 10 minutes to remove excess water from ginseng.

[0037] Cool the heat-treated ginseng to below 90°C, open the exhaust valve to inject air, and continue to repeat the above operation twice.

[0038] Grinding the heat-treated ginseng into particles with a diameter of no more than 1 mm, adding 80 vol% ethanol after drying, and heating to fully extract ginsenosides. Filter out solid particles above 500 microns through filter paper, and collect the ethanol solution through the filter paper, which is called heat-treated saponin ethanol solution.

[0039] Concentrate the heat-treated saponin ethanol so...

Embodiment 2

[0040] Example 2 Moja-3 strain fermentation (45 ℃) produces active saponin

[0041] Get the ginsenosides containing active PPDs such as Rg3 prepared in Example 1, after drying, get 24 grams for the following experiments.

[0042] First, the Mojia-3 strain was cultivated in Tryptic Soy Broth (TSB) at a temperature of 45° C. for not less than 16 hours. Take 100 ml of culture solution and add it to 1000 ml of fresh TBS for subculture, and incubate at 45°C for 60 minutes. After the incubation was completed, 100 ml of 10% (w / v) active PPD suspension was added with a hose pump at a rate of 20 ml / min. Cultivate again at 45° C. for 6 hours to obtain CKS solution.

[0043] The CKS solution was centrifuged to obtain the supernatant, which was called solution A. The precipitate after centrifugation was pooled and redissolved in a small amount of ethanol. Filter, take the filtrate, and concentrate to obtain a CKS solution with a higher concentration. The CKS solution was freeze-dried...

Embodiment 3

[0045] Example 3 Bp-2 fermented (55°C) to produce active saponins

[0046] Get the ginsenosides containing active PPDs such as Rg3 prepared in Example 1, after drying, get 24 grams for the following experiments.

[0047] First, culture the Bp-2 strain with tryptic soybean broth (TSB) at a temperature of 45° C. for not less than 16 hours. Take 100 ml of culture solution and add it to 1000 ml of fresh TBS for subculture, and incubate at 55°C for 60 minutes. After the incubation was completed, 100 ml of 10% (w / v) active PPD suspension was added with a hose pump at a rate of 20 ml / min. Cultivate again at 55° C. for 6 hours to obtain CKS solution.

[0048] The CKS solution was centrifuged to obtain a supernatant, called solution B. Dissolve the precipitate with a small amount of ethanol, filter, take the filtrate, and concentrate to obtain a CKS solution with a higher concentration. The CKS solution was freeze-dried to yield 0.0645 g of CKS (CKS primary reaction).

[0049] Con...

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Abstract

The invention relates to a method for preparing active ginsenoside. The method comprises the steps of treating ginseng with hot steam, and then conducting pressing and ginsenoside extraction with ethyl alcohol, so as to prepare active ginsenoside constituents. During fermentation, two strains (bacillus mojavensis KJS-3 and bacillus polyzyme KJS-2) are treated by means of two temperature steps (45 DEG C and 55 DEG C) in sequence. Saponin solution obtained through centrifugation is separated, and supernate is reserved. Sediment standing is conducted with ethyl alcohol again, and then filtration is conducted. Filtrate is concentrated, frozen and dried to obtain active ginsenoside. By the adoption of the method, high-yield active ginsenoside containing the compound CK can be obtained. Saponin prepared through fermentation can be used in food.

Description

technical field [0001] The invention relates to a preparation method of active ginsenoside. The invention aims at preparing active ginseng in batches. Active ginseng has a high absorption capacity in the human body. The invention is also relevant to methods of food production. Background technique [0002] Ginseng in the present invention refers to Korean ginseng, wild ginseng, cultivated wild ginseng, Chinese ginseng (notoginseng ginseng), American ginseng, and raw sun-dried ginseng, or a combination of two or more ginsengs. [0003] Ginsenosides and non-saponins include ginsenosides, polysaccharides, amino acid derivatives, and phenolic compounds. The substance has good pharmacological activity. It plays an important role in scavenging free radicals, anti-tumor, blood pressure regulation, lipid-lowering and anti-liver damage. [0004] Ginsenosides are divided into two categories: PPD (protopanaxadiol) and PPT (protopanaxatriol). PPD-type saponins have a variety of di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/20C12R1/07
CPCC12P33/20
Inventor 姜在璿欧黎虹
Owner 武汉虹睿生物科技开发有限公司
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