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Trichoderma for preventing and controlling cotton verticillium wilt and application of trichoderma

A technology of Trichoderma and Trichoderma longibranchus, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of chemical pesticides polluting the environment, easy to produce drug resistance, etc., and achieve high control effect

Active Publication Date: 2015-04-01
HAINAN JINYUFENG BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Control effect Compared with other control methods, chemical treatment has the characteristics of simple operation, large scale, quick effect, and obvious effect, but long-term use of chemical pesticides also has problems such as polluting the environment and easily producing drug resistance.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0030] Wherein, as a particularly preferred embodiment of the present invention, based on the total weight of the Trichoderma liquid fermentation medium, calculated by weight ratio, the Trichoderma liquid fermentation medium contains 2.0-3.5% starch, 1.0- 2.0% yeast powder, 4.0-8.0% corn steep liquor, 0.1-0.5% CaCO 3 , 0.01-0.02% ZnSO 4 and 0.01-0.03% MgSO 4 . Wherein, the culture medium may also contain a balance of water and / or other components. In this preferred mode, on the one hand, Trichoderma can be allowed to grow rapidly during the fermentation process to produce a sufficient number of chlamydospores; application in disease control.

[0031]Among them, as a particularly preferred embodiment of the present invention, the liquid fermentation medium of Bacillus cereus and Bacillus mogaphus contains 1-1.5% corn starch, 1.0-1.5% peanut cake powder, 0.2-0.5% ammonium sulfate, 0.1-0.4% calcium carbonate, 0.02-0.05% magnesium sulfate and 0.01-0.05% sodium chloride. Wher...

Embodiment 1

[0039] This embodiment is used to illustrate the cultivation of Trichoderma of the present invention and the preparation of microbial inoculum.

[0040] The Trichoderma longibrachiatum (Trichoderma longibrachiatum) with the preservation number of CGMCC No.9727 is inoculated into the activated medium PDA medium (get the potato 20g after peeling and boil for 20 minutes to get the juice, and the juice is made into 2g glucose, 1.5g agar and water) 100ml, pH6.8-7.0, packed into 2 eggplant bottles, 50ml each, sterilized and made on a slant), and cultivated at 30°C for 6 days. The Trichoderma obliquus bacterial classification that will activate is inoculated to the liquid fermentation medium of sterilization (by weight, containing 2.5% starch, 1.5% yeast powder, 5.0% corn steep liquor, 0.4% CaCO 3 , 0.01% ZnSO 4 , 0.03% MgSO 4 ), cultivated at 28°C for 132 hours, until 90% of mycelia formed chlamydospores, and obtained Trichoderma liquid.

[0041] Centrifuge 5000mL of the above-me...

Embodiment 2

[0043] This embodiment is used to illustrate the preparation of the composite microbial bacterial agent of the present invention.

[0044] Inoculate Bacillus cereus (Bacillus cereus) with product number ACCC 11109 on the slant of LB medium (containing 10g / L tryptone, 5g / L yeast extract, 10g / L NaCl and 15g / L agar) , cultivated at 36°C for 15 hours to obtain activated strains. Inoculate the liquid fermentation medium (by weight, containing 1% cornstarch, 1.5% peanut powder, 0.5% ammonium sulfate, 0.4% calcium carbonate, 0.03% magnesium sulfate, 0.02% chlorine sodium chloride), cultured at 35°C until the concentration of viable bacteria was 5-10×10 9 CFU / mL to obtain Bacillus cereus bacterial liquid.

[0045] Inoculate the LB medium (containing 10g / L tryptone, 5g / L yeast extract, 10g / L NaCl and 15 / L agar) slant surface with Bacillus mojavensis with product number ACCC 01174 and cultured at 35°C for 12 hours to obtain activated strains. Inoculate the liquid fermentation medium...

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PUM

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Abstract

The invention provides Trichoderma longibrachiatum with a preservation number of CGMCC No.9727. The invention further provides a compound microorganism microbial agent. The compound microorganism microbial agent comprises the following active ingredients: Trichoderma longibrachiatum, Bacillus cereus and Bacillus mojavensis. The invention further provides application of Trichoderma longibrachiatum or the compound microorganism microbial agent in prevention and treatment of cotton verticillium wilt. According to the technical scheme, the cotton verticillium wilt can be effectively prevented and controlled.

Description

technical field [0001] The invention relates to the field of microorganism applications, in particular to a Trichoderma longibrachiatum, microbial inoculants containing the Trichoderma and their application in the control of Verticillium wilt in cotton. Background technique [0002] Cotton verticillium wilt is one of the main diseases that harm cotton production. Verticillium wilt has a great impact on the yield and quality of cotton. Generally, the yield is reduced by 10% to 30%. In severe cases, the yield reduction can reach more than 80%, or even no harvest. Cotton verticillium wilt can infect and damage cotton throughout its growth period. Verticillium wilt usually appears in diseased plants one month after sowing. Due to the influence of cotton variety disease resistance, pathogenicity of pathogenic bacteria and environmental conditions, Verticillium wilt presents different symptom types. [0003] The causative agent of Verticillium wilt in cotton is Verticillium dahli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/20A01N63/04A01P3/00C12R1/885C12R1/085C12R1/07
CPCA01N63/10A01N63/30C12N1/145C12N1/205C12R2001/14C12R2001/20C12R2001/885
Inventor 李梅蒋细良刘政孙艳
Owner HAINAN JINYUFENG BIOLOGICAL ENG
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