Production method of endophytic bacillus mojavensis solid inoculant and application of production method

A technology of Bacillus virulica and solid microbial agent, applied in the field of microbiology, can solve the problems of difficult transportation and storage of liquid fermentation bacteria liquid, and achieve the effects of good environmental protection, not easy to produce drug resistance, safety, and convenient use

Inactive Publication Date: 2017-06-09
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a production method of endogenous Mojave bacillus solid bacterial agent, to solve the problems of storage difficulties, difficult transportation and secondary fermentation in the existing liquid fermentation liquid

Method used

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  • Production method of endophytic bacillus mojavensis solid inoculant and application of production method
  • Production method of endophytic bacillus mojavensis solid inoculant and application of production method
  • Production method of endophytic bacillus mojavensis solid inoculant and application of production method

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Effect test

Embodiment 1

[0019] Embodiment 1: microbial bacterial agent

[0020] Its active ingredient is Bacillus mojavensis (Bacillus mojavensis) QLY002, the thalline of CGMCC No.8905, wherein, contain the mojave bacillus 4% by weight percentage, matrix balance, matrix is ​​made up of following material: bran Skin accounted for 26.9%, corn stalk powder 20.9%, rice husk powder 20.9%, cornmeal 17.9%, soybean meal 13.4%, glucose 20g / kg, urea 10g / kg, ferrous sulfate 15g / kg. The number of live bacteria of Mojave bacillus was 3.61×10 12 CFU / mL.

Embodiment 2

[0021] Example 2: The preparation method of microbial bacterial agent, inserting Bacillus mojave into the substrate for fermentation, the material-to-water ratio is 1:1.2, the insertion amount is 4%, pH7.0, the fermentation time is 30h, and the fermentation temperature The temperature is 34°C, and the microbial inoculum is prepared for use as pesticide or fertilizer.

Embodiment 3

[0022] Embodiment 3: microbial inoculum, its active ingredient is the thalline of Mojave bacillus (Bacillus mojavensis) QLY002, CGMCC No.8905, wherein, contains the mojave bacillus 8% by weight percentage, the balance of matrix , the matrix is ​​composed of the following substances: bran accounts for 26.9%, corn stalk powder 20.9%, rice husk powder 20.9%, cornmeal 17.9%, soybean meal 13.4%, glucose 20g / kg, urea 10g / kg, ferrous sulfate 15g / kg. The number of live bacteria of Mojave bacillus was 3.43×10 13 CFU / mL.

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Abstract

The invention discloses a production method of an endophytic bacillus mojavensis solid inoculant and the application of the production method, belongs to the field of microorganism, and aims to solve the problems of difficulty in storage, uneasiness in transportation and secondary fermentation of the conventional liquid fermented bacteria solution. The method comprises the following steps: implanting bacillus mojavensis strain into a solid substrate, so as to be fermented, with the implanting amount of 4 to 12 percent, pH value of 7.0 to 8.0, the fermentation time of 30 to 42h, and the fermentation temperature of 34 to 42 DEG C. The microorganism inoculant fermented through agricultural waste solid is easy to store and transport and convenient to use, and has obvious advantages. The control effect of endophytic bacillus mojavensis to cucumber fusarium wilt and capsicum fusarium wilt is greater than 61 percent, so that the defects of poor environmental protection property, easiness in insecticide resistance production and poor safety, and the endophytic bacillus mojavensis has the advantages of good environmental protection property, uneasiness in insecticide resistance production, good safety and growth promotion (fertilizer) functions, meets the national requirements of pesticide reducing and chemical fertilizer reducing, and is also beneficial to environmental protection.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a production method and an application of an endogenous Mojave bacillus solid microbial agent. Background technique [0002] After entering the 21st century, based on the pollution of chemical pesticides to the environment and pesticide residues, with the proposal and practice of the concept of "public plant protection, green plant protection", the research and development of biological pesticides has become a hot spot in my country's biological industry, agricultural research and application, and has been listed As a major research field and direction of the national medium and long-term scientific and technological development plan, green plant protection has become an important demand and inevitable trend of national development. Among them, the research on plant endophytic bacteria as antagonistic bacteria has attracted the attention of many scholars. Plant endophyti...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
CPCA01N63/10C12N1/20
Inventor 杨成德陈秀蓉薛莉牛小丽
Owner GANSU AGRI UNIV
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