Bacillus mojavensis, microbial inoculant and application of bacillus mojavensis and microbial inoculant

A technology of Bacillus mogavvii and microbial inoculum, which is applied in the field of agricultural biology and can solve problems such as poor saline-alkali effect

Active Publication Date: 2014-04-23
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the defect that the existing microbial strains have poor salinity-alkali removal effect, the pr

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0019] Wherein, the preparation method of the microbial agent may include: respectively inoculating Bacillus mogaf, Bacillus subtilis, Bacillus licheniformis and Bacillus amyloliquefaciens in the culture medium for culturing. Wherein, Bacillus mogaf, Bacillus subtilis, Bacillus licheniformis and Bacillus amyloliquefaciens can be respectively cultivated in separate culture systems, and the microorganisms obtained by cultivating them can be mixed in proportion. Preferably, by cultivating in separate culture systems and mixing after culturing, the total number of live bacteria per gram of microbial agent obtained is 2-20×10 7 CFU.

[0020] According to the present invention, the cultivation method of Bacillus subtilis is not particularly limited and known to those skilled in the art, for example, Bacillus subtilis can be cultivated in seed medium (LB medium) to a bacterial density OD 600 The value is 0.6-0.8, to obtain the bacterial liquid, and then inoculate 2-5 parts by weigh...

Embodiment 1

[0032] This example is used to illustrate the Bacillus mogafii of the present invention and its properties.

[0033] Inoculate the Bacillus mojavensis with the preservation number CCTCC NO.M2013499 into LB medium (containing tryptone 10g / L, yeast extract 5g / L, NaCl10g / L) and cultivate to the bacterial density OD 600 The value is 0.6-0.8, and the bacterial liquid is obtained.

[0034] NaCl was added to the LB medium to adjust the concentration of NaCl to 220 g / L, and NaOH was added to adjust the pH value to 9.5 to obtain a saline-alkali stress medium.

[0035] Add 3mL of the above bacterial solution into the saline-alkali stress medium to make the initial OD 600 0.1, at 30°C, shake culture at 180 rpm, take samples every 4h to determine the bacterial density in the fermentation broth (OD 600 ). The results showed that the OD of the fermentation broth at 0, 4, 8, 12, 16, 20, and 24 hours 600 They are 0.1, 0.12, 0.16, 0.23, 0.38, 0.55 respectively. This shows that the Bacillu...

Embodiment 2

[0045] This embodiment is used to illustrate the microbial inoculant of the present invention and its performance.

[0046] (1) Inoculate 5 parts by weight of Bacillus subtilis bacteria liquid (purchased from China Agricultural Microorganism Culture Collection Center) in 100 parts by weight of culture medium (glucose 5g, soybean meal 30g, peptone 2g, distilled water 1000ml, pH7.0-7.2) And the number is ACCC03221, cultivated in the same LB medium as above to the bacterial density OD 600 value of 0.6-0.8 to obtain the bacterial liquid), cultivated at 37°C, took samples during the cultivation and observed them by direct counting under microscope until the number of viable Bacillus subtilis was 2×10 7 CFU / gram of medium.

[0047] (2) Inoculate 5 parts by weight of Bacillus licheniformis solution (purchased From the China Agricultural Microbiology Culture Collection Center and the number is ACCC02698, cultured in the same LB medium as above to a bacterial density of OD 600 The v...

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PUM

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Abstract

The invention provides bacillus mojavensis with the preservation number of CCTCC NO. M2013499. The invention also provides a microbial inoculant. The microbial inoculants comprises a medium and bacteria, wherein the bacteria comprise bacillus mojavensis with the preservation number of CCTCC NO. M2013499, and also comprises bacillus subtilis, bacillus lincheniformis and bacillus amyloliquefaciens. The invention also provides application of bacillus mojavensis as described above and the microbial inoculants as described above to saline land improvement. The invention also provides application of bacillus mojavensis as described above and the microbial inoculants as described above to increase plant drought tolerance. By employing the above technical scheme, salts and alkalis can be relatively effectively removed, for example, the soil desalting rate is improved by 55% or more.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a bacillus mogaf, a microbial agent and applications thereof. Background technique [0002] Land salinization is a serious problem affecting agricultural production and ecological environment. About 20% of the arable land and nearly 50% of the irrigated farmland in the world are seriously affected by the high concentration of salinity in the soil. In China, there are about 100 million mu of secondary salinized farmland, accounting for 10% of the total cultivated land. How to really improve and effectively utilize saline-alkali land and improve the economic, social and ecological benefits of agricultural and forestry production is a major concern of modern agriculture and forestry. [0003] In the field of saline-alkali land improvement research at home and abroad, the agricultural improvement measures of "planting rice to improve alkali", the engineering improvement me...

Claims

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Application Information

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IPC IPC(8): C12N1/20C09K17/00C12R1/07C12R1/125C12R1/10C09K101/00
Inventor 阮志勇王彦伟宋金龙孔德龙赵秉强李燕婷王慧敏陈小蓉刘艳伟刘小飞
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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