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Pear blight antagonistic bacterium, biological bactericide and application thereof

An antibacterial and dry disease technology, applied in the directions of biocide, application, biocide, etc., can solve problems such as destroying ecology, affecting food safety, environmental pollution, etc., and achieving the effect of increasing production, major economic benefits, and human and animal safety.

Active Publication Date: 2019-05-28
武威市林业科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, chemical control methods are mainly used for the prevention and treatment of pear blight, and the extensive use of chemical pesticides is likely to cause negative impacts such as environmental pollution, ecological damage, and food safety.
There is no research report on the control of Bacillus mojavensis in pear blight

Method used

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  • Pear blight antagonistic bacterium, biological bactericide and application thereof
  • Pear blight antagonistic bacterium, biological bactericide and application thereof
  • Pear blight antagonistic bacterium, biological bactericide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] This example mainly screens and identifies antagonistic bacteria. The specific screening and identification process is as follows:

[0021] 1. Test method

[0022] (1) Strain isolation

[0023] The dilution coating method was used to separate the antagonistic bacteria in the soil. The soil sample was collected from the pear orchard of the Wuwei Academy of Forestry Sciences. 10g of the soil sample was air-dried and sieved (2mm mesh), put into a 250mL Erlenmeyer flask, added 90mL sterile water in a water bath at 80℃ for 30min, and then fully shaken for 30min. Dilute by 10 times. Before the suspension settles and stand still, suck 10mL into another 90mL Erlenmeyer flask, shake well, and dilute to a concentration of 10 -1 g / mL, 10 -2 g / mL, 10 -3 g / mL, 10 -4 g / mL, 10 -5 g / mL, 10 -6 g / mL suspension. Finally take 10 -4 g / mL, 10 -5 Add 0.5 mL of the suspension with a concentration of g / mL to the surface of the prepared PDA medium, spread it on the plate, and cultivate at a constant ...

Embodiment 2

[0040] This example mainly tested the antagonistic mechanism of strain LKYHC-2.

[0041] 1. Test method

[0042] Spot the antagonistic strain LKYHC-2 cultivated for 1 d on casein medium, starch medium, and carboxymethyl cellulose medium, inoculate 3 strains per dish, culture at 30°C for 5 days, and observe whether there is a transparent circle around the colony , The starch medium should be dripped with a few drops of iodine solution before observation; the determination of cellulase activity should be stained with 1g / L Congo red for 10-15min, then the dye solution should be discarded, and then soaked with 1mol / L NaCl for 15min .

[0043] Wherein, the casein medium (1000 mL): 20 g of skimmed milk powder, 5 g of yeast extract, 5 g of glucose, 16 g of agar, 1000 mL of water, pH 7.0.

[0044] The starch medium (1000 mL): 20 g of soluble starch, 10 g of peptone, 5 g of glucose, 5 g of NaCl, 5 g of beef extract, 16 g of agar, 1000 mL of water, pH 7.0.

[0045] The carboxymethyl cellulose m...

Embodiment 3

[0049] In this example, a field test was performed on the strain LKYHC-2.

[0050] 1. Test method

[0051] In April 2017, the pathogen of pear dry blight was inoculated into the stems of young pear trees. In July, a field control experiment of strain LKYHC-2 was carried out. Mancozeb and water treatment were selected as controls. Thoroughly scrape the diseased tissues on the branches of the pear before applying the medicine. The scraped area exceeds the diseased spot by 0.5~1.0cm, and then apply LKYHC-2 fermentation broth, mancozeb and water, once every 10 days for a total of Three times, survey in April of the following year, statistics of cure rate and recurrence rate of disease spots on pear branches of each treatment.

[0052] The preparation of the LKYHC-2 fermentation broth: inoculate the antagonistic bacillus LKYHC-2 into the NB liquid medium, culture at 28°C and 150r / min shaking for 3 days to prepare the LKYHC-2 fermentation broth, dilute with sterile water to a concentratio...

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Abstract

The invention discloses a pear blight antagonistic bacterium LKYHC-2 and belongs to bacillus mojavensis. The length of 16S rDNA nucleotide sequence of the antagonistic bacterium is 1390bp, the antagonistic bacterium is preserved in China General Microbiological Culture Collection Center on November 29th 2018, and a preservation number of the bacterium is CGMCC No.16838. A bacterial colony of the antagonistic bacterium LKYHC-2 is milk white on an LB solid culture medium, the middle of the bacterial colony is concave, the concave is not obvious, the bacterial colony is not transparent, the bacterial colony has wrinkles, the bacterial colony can generate red pigment of gram positive bacterium, and the gram positive bacterium is in a rod shape and has spores. The antagonistic bacterium LKYHC-2can be applied to preventing and treating pear blight, and a relative inhibition rate of the antagonistic bacterium to hypha of pathogenic bacteria of the pear blight is 88.84%; after the antagonistic bacterium LKYHC-2 is applied to fields to prevent and treat the pear blight, a scab cure rate is 66.67%, and a scab relapsing rate is 13.33%. The antagonistic bacterium is safe to environment, humanand livestock and solves the problem of drug resistance generated by using chemical agents to prevent and treat the pear blight for a long time.

Description

Technical field [0001] The invention relates to the field of strains, in particular to an antagonistic antibacterial and biological agent for pear blight and its application. Background technique [0002] my country is one of the three origin centers of pear cultivation in the world (China Center, Central Asia Center and Near East Center). It has a cultivation history of more than 3000 years. It is the world's largest producer and consumer of pears, accounting for about 20% of the world's population. , Producing and consuming 70% of the world’s pears, it can be said that pears play a pivotal role in my country’s fruit industry system. However, with the development of the pear industry, the occurrence of various pear diseases has seriously affected the quality and yield of pears and the healthy development of my country's pear industry. The main diseases of pears that are more common in China are black spot, scab, ring pattern, pear rust, etc. The prevention and control are mainly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
Inventor 何彩张军刘伟王多文金娜王鑫史星雲李强张鹏曹虎郭艳兰张勤德牟德生赵连鑫段爱莉李栋董存元
Owner 武威市林业科学研究院
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