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Bacillus mojavensis for preventing and treating root rot of cotton in seedling stage and application of Bacillus mojavensis

A Bacillus, Mohaiwei technology, applied in the application, bacteria, fungicides and other directions, can solve the problem of not finding root rot diseases of Bacillus Mohaiwei, and achieve difficult drug resistance, high efficacy and long-lasting effect. long effect

Active Publication Date: 2020-05-05
新疆农业科学院核技术生物技术研究所(新疆维吾尔自治区生物技术研究中心) +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the bacillus that is used to prevent and treat crop diseases mainly contains Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus megaterium etc., after searching, do not find the Bacillus mohaiwei of the present invention aspect the root rot class disease of preventing and treating cotton seedling stage. to report

Method used

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  • Bacillus mojavensis for preventing and treating root rot of cotton in seedling stage and application of Bacillus mojavensis
  • Bacillus mojavensis for preventing and treating root rot of cotton in seedling stage and application of Bacillus mojavensis
  • Bacillus mojavensis for preventing and treating root rot of cotton in seedling stage and application of Bacillus mojavensis

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Example 1 Isolation and screening of Bacillus mohewei HWt34

[0030] (1) Isolation of strain HWt34

[0031] Strain HWt34 was isolated from the cotton field soil in Shihezi by the Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences. Soil samples from cotton fields in Shihezi were collected and brought back to the laboratory for separation and purification using the soil dilution method. Taking cotton blight as the target, the biocontrol bacteria were screened through plate confrontation method, pot test method and field plot test. A strain with obvious control effect on cotton blight was screened out and named as HWt34.

[0032] (2) Taxonomic identification of strain HWt34

[0033] ① Identification and classification by 16S rDNA sequence

[0034] Using the genomic DNA of HWt34 as a template, PCR amplification was carried out with bacterial universal primers 27F and 1492R to obtain PCR amplification products, wherein the primer sequences are:

[...

Embodiment 2

[0045] The preparation of embodiment 2HWt34 wettable powder

[0046] (1) Strain activation: put the preserved bacterial strain HWt34 on the LB plate medium (its composition and weight ratio are: yeast extract 5g, peptone 10g, NaCl 5g, agar powder 15g, water 1000ml, pH7.4~7.6 ), activated at 30°C, picked a single colony and cultured it on LB slant medium at 30°C for 24 hours to obtain the activated strain;

[0047] (2) Preparation of seed liquid: make LB liquid medium according to conventional methods (its composition and weight ratio are: tryptone 10g, yeast extract 5g, sodium chloride 5g, water 1000ml), and put it in a 250ml Erlenmeyer flask Add 100ml of LB culture solution, and sterilize with high-pressure damp heat. After the temperature drops to room temperature, insert an inoculation loop in each bottle into the above-mentioned activated strain in step (1), and oscillate at 30°C and shaker speed 150rpm Cultivate for 24 hours to obtain seed liquid;

[0048] (3) Preparati...

Embodiment 3

[0052] Embodiment 3 Mohaiwei bacillus HWt34 is to the inhibition of R. solani mycelia growth of cotton

[0053] In March 2019, the plant disease laboratory of the Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences conducted an inhibition test of Bacillus mohaiwei HWt34 on the mycelial growth of R. solani.

[0054] Rhizoctonia solani for testing: The strain of Rhizoctonia solani was collected from a cotton field in Xinjiang. It was isolated and purified in the laboratory and identified as Rhizoctoni solani (Rhizoctoni asolani). The pathogenicity test showed strong pathogenicity.

[0055] Tablet standoff test:

[0056] Rhizoctonia solani is activated in the PDA substratum, cultivates after 3 days in the colony edge area, punches with the hole puncher (0=6mm) and makes the bacterium cake, the bacterium cake adopts cross method to be inoculated to the PDA medium center, Then the Bacillus mohewei HWt34 activated in the step (1) of Example 2 was inoculated wit...

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Abstract

The invention discloses Bacillus mojavensis for preventing and treating root rot of cotton in a seedling stage and application of the Bacillus mojavensis, belonging to the technical field of agricultural biological prevention and treatment. The Bacillus mojavensis HWt34 disclosed by the invention provides a novel and efficient microorganism for preventing and treating root rot of cotton in the seedling stage; a Bacillus mojavensis wettable powder and a biological fertilizer disclosed by the invention are high in pesticide effect on cotton root rot, have an average control effect of 78.43% or above, and thus have broad-spectrum bactericidal effect; and the Bacillus mojavensis wettable powder and the biological fertilizer are long in lasting period during preventing and treating of the rootrot of the cotton in the seedling stage, do not easily generate drug resistance, and have an obvious promoting effect on cotton growth.

Description

technical field [0001] The invention relates to the technical field of agricultural biological control, in particular to a bacillus mohaiwei for preventing and controlling root rot of cotton seedlings and its application. Background technique [0002] Cotton seedling root rot diseases include blight (Rhizoctoni asolani Kuhn), damping-off [Pythium aphanidermatum (Eds.) Fitz], anthracnose [Colletotrichum gossypii Southw.], red rot [Fusarium moniliforme (Var.) intermedium Neish et Leggett] and black root rot [Thilaviopsis basicola (Berk.et Br.) Ferravis], due to continuous cropping all year round, frequent extreme low temperature weather in early spring and other factors, the incidence of cotton blight in Xinjiang is becoming more and more serious. [0003] The prevention and control of cotton root rot at the seedling stage is mainly through chemical pesticide spraying, coating and other measures. However, chemical pesticides have disadvantages such as polluting the environment...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N25/14A01N63/22A01P3/00C12R1/07
CPCC12N1/20A01N25/14A01N63/00C12R2001/07C12N1/205Y02W30/40
Inventor 刘海洋雷斌努尔孜亚·亚力买买提李进张仁福周小云杜鹏程姚举张军高熊黎黎王伟顾爱星隋建中王玮玮刘梦丽梁晶
Owner 新疆农业科学院核技术生物技术研究所(新疆维吾尔自治区生物技术研究中心)
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