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Microbial composition, microbial preparation and method for sandy soil improvement

A technology of microbial composition and microbial preparation, which is applied in the field of microorganisms, can solve the problems of unreported and stimulated co-cultivation of Bacillus bacteria, and achieve the effects of achieving sustainable development and improving soil physical and chemical properties.

Active Publication Date: 2019-09-10
NORTHWEST INST OF ECO ENVIRONMENT & RESOURCES CAS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, the research and development of microbial soil improvement in sandy areas is still in the exploratory stage. The existing research and development of related technologies aim at adding fertilizers to stimulate microbial growth and metabolism, which has initially confirmed the positive significance of microbial soil improvement technology in improving soil physical and chemical properties.
[0003] At present, there is no report on the application of co-cultivation of Bacillus bacteria in soil improvement in sandy areas

Method used

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  • Microbial composition, microbial preparation and method for sandy soil improvement
  • Microbial composition, microbial preparation and method for sandy soil improvement
  • Microbial composition, microbial preparation and method for sandy soil improvement

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Isolation, Screening and Identification of Bacillus Bacteria

[0044] (1) Sample collection:

[0045] On June 29, 2018, the biological soil crusts in the artificial sand-fixing vegetation area of ​​Shapotou, Tengger Desert were collected.

[0046] (2) Medium:

[0047] a) LB medium: tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, distilled water 1000mL, pH7.0-7.2 (add 2% agar powder for solid medium).

[0048] b) Amylase selective medium: starch 2g / L, beef extract 5g / L, glucose 5g / L, tryptone 10g / L, sodium chloride 5g / L, agar powder 20g / L, distilled water 1000mL, pH 7.0- 7.2.

[0049] c) Protease selective medium: casein 5g / L, glucose 10g / L, yeast powder 10 g / L, dipotassium hydrogen phosphate 1g / L, potassium dihydrogen phosphate 0.5g / L, magnesium sulfate 0.1g / L, Agar powder 20g / L, distilled water 1000mL, pH 7.0-7.2.

[0050] d) Cellulase selective medium: sodium carboxymethylcellulose 20g / L, glucose 5 g / L, tryptone 2.5g / L, disodium hydrogen phosphate 2.5g / L...

Embodiment 2

[0066] Determination of Polysaccharide Production Ability of Co-cultured Bacillus Bacteria

[0067] a) The above-mentioned Bacillus strains B3, B5 and B8 were inoculated into LB medium respectively, cultured on a shaker (230 rpm) at 30° C. for 12 hours, and set aside.

[0068] b) Joint culture: transfer the above seed liquid to the joint culture medium (containing 2% industrial yeast powder, 3% industrial glucose , MgSO 4 1%, pH 8.2-8.5), cultivated at 30°C and 230rpm for 18h as a fermentation broth, and set aside;

[0069] Separate culture of strain B3: transfer the seed solution of the above-mentioned strain B3 to the above-mentioned combined medium according to the inoculum amount of 13%, and cultivate it at 30°C and 230 rpm for 18 hours as a fermentation liquid, and set aside;

[0070] Separate culture of strain B5: transfer the seed solution of the above-mentioned strain B5 to the joint medium according to the inoculum amount of 13%, and cultivate it at 30°C and 230rpm...

Embodiment 3

[0076] Soil Improvement of Co-cultivation of Bacillus Bacteria

[0077] a) The above-mentioned Bacillus strains (B3, B5 and B8 strains) were inoculated into LB medium respectively, and cultured on a shaker (230 rpm) at 30° C. for 12 hours as a seed solution.

[0078] b) The above seed liquid is transferred to the joint culture medium (2% of industrial yeast powder, 3% of industrial glucose, MgSO 4 1%, pH 8.2-8.5), cultivated at 30° C. and 230 rpm for 18 hours as a fermentation broth, and set aside.

[0079] c) The number of effective viable bacteria measured by the dilution coating method is respectively Bacillus subtilis B3 2.16 × 10 9 CFU / mL, Bacillus mohewei B5 1.19×10 9 CFU / mL and Bacillus amyloliquefaciens B8 1.61×10 9 CFU / mL. Dilute the total effective number of viable bacteria in its fermentation broth to 1.0 × 10 6 CFU / mL, according to 1.0×10 9 CFU / m 2 The amount is evenly sprayed on the surface of the sand area.

[0080] d) The result is as follows Figure 6...

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Abstract

The invention discloses a microbial composition, microbial preparation and method for sandy soil improvement, and relates to the technical field of microbes. The microbial composition for sandy soil improvement contains bacillus subtilis, bacillus mojavensis and bacillus amyloliquefaciens. The microbial composition is applied to a sandy area, not only can make grains of sand agglomerated well andbe kept in a relatively stable state, but also can effectively improve the physicochemical properties of the soil, can be widely applied to sandy soil restoration, and is conductive to achieving the sustainable development of the sandy area.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a microbial composition, a microbial preparation and a method for soil improvement in sandy areas. Background technique [0002] How to improve soil fertility is one of the important technical problems in the current human struggle with soil improvement. Compared with soil fertilization, seedling planting and physical reconstruction technology, microbial soil improvement method, as a new soil improvement technology, has the advantages of less pollution, low price, short time-consuming, strong adaptability, and small amount of engineering, and has been accepted by the international community. It is generally recognized that it is a measure with the most development potential to fundamentally restore the soil ecosystem in the future. However, at present, the research and development of microbial soil improvement in sandy areas is still in the exploratory stage. The existing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K17/14C09K17/32C12R1/125C12R1/07C09K105/00
CPCC09K17/14C09K17/32C09K2105/00C12N1/20
Inventor 赵丽娜刘玉冰王增如黄文广张宇王蕾罗晓玲
Owner NORTHWEST INST OF ECO ENVIRONMENT & RESOURCES CAS
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