Lactobacillus acidophilus and screening method and application thereof and microbial inoculum

A technology for screening Lactobacillus acidophilus and its screening method, which is applied in the field of Lactobacillus acidophilus and its screening, bacterial agents, can solve the problem that the detoxification effect cannot meet the actual needs, etc., and achieve the purpose of inhibiting the reproduction of pathogenic bacteria in the gastrointestinal tract of animals and promoting intestinal The effects of microbial balance, strong acid production and ability to inhibit harmful bacteria

Active Publication Date: 2018-05-01
FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the detoxification of rapeseed meal has been achieved by adding microbial agents in the prior art, the detoxification effect of the existing microbial agents cannot meet the actual needs

Method used

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  • Lactobacillus acidophilus and screening method and application thereof and microbial inoculum
  • Lactobacillus acidophilus and screening method and application thereof and microbial inoculum
  • Lactobacillus acidophilus and screening method and application thereof and microbial inoculum

Examples

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Embodiment 1

[0049] Example 1 Screening and identification of Lactobacillus acidophilus

[0050] This implementation provides a screening method for Lactobacillus acidophilus, the specific steps are:

[0051] 1) Isolation of glucosinolate-degrading bacteria

[0052] Take 20g of fermented corn silage and inoculate it into a 250ml Erlenmeyer flask filled with 120ml of sterile water, fully shake it on a shaker at 37°C and 200r / min for 20min, then let it stand for 30min, and take 20ml of the supernatant and inoculate it into rapeseed meal In the fermentation medium, culture at a constant temperature of 37°C for 48 hours, stir evenly with a sterilized glass rod, take out 5g and shake and dilute with sterile water, and take the supernatant to make the stock solution of the strain;

[0053] 2) Primary screening of glucosinolate-degrading bacteria

[0054] Take the stock solution of the above strains and press 10 -1 、10 -2 、10 -3 、10 -4 Doubly diluted and spread on the MRS+glucosinolate soli...

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Abstract

The invention relates to the technical field of microbes, in particular to lactobacillus acidophilus and a screening method and application thereof and a microbial inoculum. The collection number of the lactobacillus acidophilus is CGMCC NO.14437; the lactobacillus acidophilus has a strong glucosinolate degrading function, and can be applied to detoxification of rapeseed meal by independently serving as a fermenting microbial inoculum; when the lactobacillus acidophilus is applied to a rapeseed meal fermenting process, the lactobacillus acidophilus can promote production of polypeptide and lactic acid and has strong abilities of producing acid and inhibiting harmful bacteria; as a broiler feed additive, the lactobacillus acidophilus can promote nutrient digestion and absorption of animals,inhibit proliferation of pathogenic bacteria in gastrointestinal tracts of the animals and promote the balance of intestinal microflora. The lactobacillus acidophilus is a facultative anaerobic microorganism, and is strong in environmental applicability, high in growth speed, tolerable to high-concentration glucosinolate and high in safety.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a lactobacillus acidophilus, a screening method and application thereof, and a microbial agent. Background technique [0002] Rapeseed meal is a kind of by-product with rapeseed oil aroma produced during the oil extraction process of rapeseed, and it is an important animal protein feed resource in my country. The crude protein content is between 35% and 45%, the amino acid content is rich and the composition is reasonable, and it is a high-quality and cheap feed protein raw material resource. However, rapeseed meal contains a series of anti-nutritional factors including glucosinolates, tannins, phytic acid, etc., and the molecular weight of protein is large, and the content of cellulose is high, which limits its addition ratio in animal diets. With the rapid development of my country's animal husbandry industry, the supply of conventional feed materials, especially protei...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C12P21/00A23K10/12A23K10/18A23K50/75A23L5/20C12R1/23
CPCA23K10/12A23K10/18A23K50/75A23L5/28C12N1/02C12N1/20C12P21/00C12R2001/23C12N1/205A23V2400/113
Inventor 刘国华吴正可常文环蔡辉益
Owner FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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