Lactobacillus acidophilus and screening method and application thereof and microbial inoculum
A technology for screening Lactobacillus acidophilus and its screening method, which is applied in the field of Lactobacillus acidophilus and its screening, bacterial agents, can solve the problem that the detoxification effect cannot meet the actual needs, etc., and achieve the purpose of inhibiting the reproduction of pathogenic bacteria in the gastrointestinal tract of animals and promoting intestinal The effects of microbial balance, strong acid production and ability to inhibit harmful bacteria
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[0049] Example 1 Screening and identification of Lactobacillus acidophilus
[0050] This implementation provides a screening method for Lactobacillus acidophilus, the specific steps are:
[0051] 1) Isolation of glucosinolate-degrading bacteria
[0052] Take 20g of fermented corn silage and inoculate it into a 250ml Erlenmeyer flask filled with 120ml of sterile water, fully shake it on a shaker at 37°C and 200r / min for 20min, then let it stand for 30min, and take 20ml of the supernatant and inoculate it into rapeseed meal In the fermentation medium, culture at a constant temperature of 37°C for 48 hours, stir evenly with a sterilized glass rod, take out 5g and shake and dilute with sterile water, and take the supernatant to make the stock solution of the strain;
[0053] 2) Primary screening of glucosinolate-degrading bacteria
[0054] Take the stock solution of the above strains and press 10 -1 、10 -2 、10 -3 、10 -4 Doubly diluted and spread on the MRS+glucosinolate soli...
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