Breeding method for pod dehiscence-resistant cytoplasmic sterile line and pod dehiscence-resistant cytoplasmic maintainer line of brassica napus

A technology of cytoplasmic sterile line and Brassica napus, which is applied in the field of breeding of anti-cleavage keratin male sterile line and maintainer line of rapeseed, and achieves the effects of excellent agronomic traits, good stability and reproducibility, and easy operation

Inactive Publication Date: 2012-02-08
HENAN ACAD OF AGRI SCI
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  • Breeding method for pod dehiscence-resistant cytoplasmic sterile line and pod dehiscence-resistant cytoplasmic maintainer line of brassica napus
  • Breeding method for pod dehiscence-resistant cytoplasmic sterile line and pod dehiscence-resistant cytoplasmic maintainer line of brassica napus
  • Breeding method for pod dehiscence-resistant cytoplasmic sterile line and pod dehiscence-resistant cytoplasmic maintainer line of brassica napus

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Embodiment Construction

[0014] 1. Improved the random collision method to identify the anti-crack angle ability of rapeseed with the anti-crack angle index

[0015] Pick 50 siliques in the middle of the main inflorescence of 10 typical individual plants at the yellow maturity stage of Brassica napus, place them in nylon mesh bags, hang them to dry, and place the siliques in an environment of 20°C and 50% relative humidity before testing. week. The siliques are then sealed and preserved. The random collision method is used for testing. The random collision instrument is a CSC-24 shaker, and the container for placing siliques is a polyethylene plastic cylinder with an inner diameter of 19 cm and a height of 14 cm, and 12 stainless steel balls with a diameter of 13 mm are placed in the container. The vibration amplitude of the shaker is 24mm. Take 20 siliques for each variety (line), shake the table at 400 times min -1 Shake for 1 min, record the number of broken siliques, and repeat 10 times. Each...

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Abstract

The present invention discloses a breeding method for pod dehiscence-resistant cytoplasmic sterile line and pod dehiscence-resistant cytoplasmic maintainer line of brassica napus, and belongs to a cultivation method for a new crop variety. With the method provided by the present invention, the detection method for pod dehiscence resistance of the rape is improved; the pod dehiscence-resistant germplasm resource is screened; the screened pod dehiscence-resistant line H155 and the cytoplasmic sterile line 077A are subjected to hybridization, and continuous 8 backcrosses to breed the cytoplasmic sterile line H155A, wherein the sterility rate of the H155A is 100%. According to the present invention, the H155A has characteristics of micro pollen at the low temperature, complete sterility at the high temperature, good agronomic characteristics, cold resistance, viral disease resistance and sclerotium disease resistance. The erucic acid content and the glucosinolate content in the H155A is consistent with the erucic acid content and the glucosinolate content in the cytoplasmic maintainer line of the H155A. The pod dehiscence-resistant cytoplasmic sterile line and the pod dehiscence-resistant cytoplasmic maintainer line of the brassica napus establish the foundation for breeding the domestic pod dehiscence-resistant cytoplasmic sterile line hybrids.

Description

Technical field: [0001] The invention relates to the breeding technology of anti-cleavage keratin male sterile line and maintainer line of rapeseed, which belongs to a new method for cultivating new varieties of crops. Background technique: [0002] Rapeseed is the main oil crop in my country, and its production and development are directly related to the market supply of edible oil. In recent years, the contradiction between the supply and demand of edible oil in our country is very prominent, so taking various measures to increase the output of rapeseed and improve the production efficiency of rapeseed is the central topic of rapeseed research. Among them, the cleavage problem of rape, which affects the yield of rapeseed and mechanical harvesting, is one of the main factors restricting the production of rapeseed at present. [0003] The cleavage angle is a biological characteristic formed in the long-term evolution of rape in order to adapt to the environment and reproduc...

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Application Information

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IPC IPC(8): A01H1/04A01H1/02
Inventor 文雁成张书芬王建平朱家成赵磊
Owner HENAN ACAD OF AGRI SCI
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