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Rape seed protein feedstuff and preparation method thereof

A technology of rapeseed protein and feed, applied in the field of feed, can solve the problems of not being able to make full use of microbial resources, destroy the original configuration of amino acids, and be unfavorable for animal feed production, and achieve environmental protection, promote digestion and absorption, and growth and development. Ease of promoting the effect of the application

Inactive Publication Date: 2009-07-29
NANJING UNIV OF FINANCE & ECONOMICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The acid or alkali added in the production of protein feed by the chemical detoxification method will hydrolyze the protein in the rapeseed meal and produce chloropropanol carcinogens, and the reaction conditions are severe, destroying the original configuration of the amino acid, and at the same time hydrolyzing the waste liquid Emissions will also cause certain environmental pollution, which is not conducive to the production of animal feed
Microbial fermentation detoxification to produce feed protein, the process is simple, and the amount of feed added is large, which can be used for large-scale production of rapeseed feed protein. role, can not make full use of microbial resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Screening of rapeseed protein feed bacteria rich in small peptides by solid-state fermentation of rapeseed meal

[0019] Fermentation strains: Aspergillus niger, Rhizopus niger, Geotrichum candidum, Aspergillus oryzae, Aspergillus usami, Mucor actinosa, Lactic acid bacteria, Candida utilis, Bacillus licheniformis and Bacillus subtilis.

[0020] Preparation of starter: After the activated strains are enlarged and cultivated according to conventional methods, the fermented seeds are diluted with distilled water to make the cell concentration reach 3×10 7 Each / mL is the starter.

[0021] The double-low rapeseed meal was pulverized to a particle size of 500 μm, and sterilized at 121° C. for 30 minutes with moist heat. In the fermenter, the starter is added according to the solid-liquid ratio of 1:1-1:2 (mass ratio), the fermentation temperature is 28°C-35°C, the ambient humidity is 80%-90%, stirring and ventilating. After 3 days of fermentation, it is dried and ...

Embodiment 2

[0023] Example 2 Preparation of Rapeseed Protein Feed Rich in Small Peptides by Solid State Fermentation of Bacillus subtilis

[0024] Incline medium: beef extract 3g, peptone 10g, NaCl 5g, agar 20g, tap water 1000mL, pH7.2-7.4.

[0025] Seed medium: beef extract 3g, peptone 10g, NaCl 5g, agar 20g, tap water 1000mL, pH7.2-7.4.

[0026] Nutrient solution composition: glucose 2.6g, KH 2 PO 4 2.6g, tap water 1000, pH 7.0.

[0027] Fermentation medium: rapeseed meal, nutrient solution.

[0028] Preparation of starter: Pick two rings of Bacillus subtilis from the activated strain culture slant and inoculate it in the seed medium, seal it with 8 layers of gauze, culture it on a shaking table at 35°C, 120r / min for 24 hours, and make the fermentation broth The bacteria concentration reaches 10 8 individual / mL. Dilute the fermentation broth with nutrient solution to make the cell concentration 3×10 7 individual / mL.

[0029] The canola meal was pulverized to a particle size of 5...

Embodiment 3

[0031] Example 3 Rapeseed protein feed rich in small peptides prepared by solid-state fermentation of Mucor radiata

[0032] Slant medium: 300g potato, 20g glucose, 20g agar, 1000mL tap water.

[0033] Plate expansion medium: 300g potato, 20g glucose, 20g agar, 1000mL tap water.

[0034] Nutrient solution composition: glucose 50g, KH 2 PO 4 2g, tap water 1000mL, pH 6.5.

[0035] Fermentation medium: rapeseed meal, nutrient solution.

[0036] Preparation of starter: inoculate Mucorra jazhi on slant culture medium and incubate at 28°C for 4 days, then inoculate on plate for expansion at 28°C, wash spores with sterile nutrient solution after 4 days, and adjust the concentration to 10 7 individual / mL.

[0037] Crush the common rapeseed meal to a particle size of 500 μm, sterilize, add a starter in a fermenter according to a solid-to-liquid ratio of 1:1.5 (mass ratio), adjust the fermentation temperature to 30°C, and the ambient humidity to 90%, stir ventilation. After 5 da...

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Abstract

The invention provides a rape seed protein feed, which can be solid powder or particles. The feed comprises the following compositions in percentage by weight: 35 to 50 percent of protein (N*6.25, butt), 25 to 33 percent of soluble nitrogen, and 10 to 20 percent of small peptide; and content of glucosinolate is 15 to 25 micromole / gram rape seed protein feed. The invention provides a method for preparing the rape seed protein feed, which comprises the following steps: fermenting crushed rape seed dregs in solid state by a food grade single strain with prolease producing capability; and sterilizing, drying and crushing a fermented culture medium substrate to obtain the rape seed protein feed. The method is friendly to environment in mild reaction condition, and can sufficiently play roles in hydrolyzing the prolease of microorganism and degrading the glucosinolate, so the method is applicable to industrial mass preparation of the rape seed protein feed with low cost.

Description

technical field [0001] The invention relates to a feed, in particular to a rapeseed protein feed obtained by using rapeseed meal as a raw material, and also to a preparation method thereof. Background technique [0002] Rapeseed is an oil crop produced in a large area in my country, and its output ranks first in the world. Rapeseed oil is also the main edible vegetable oil for Chinese residents. The by-products produced in the oil processing process are huge. The output of rapeseed meal alone exceeds 1,000 per year. tons. As the main by-product of the oil processing industry, rapeseed meal contains 35% to 50% of crude protein, and the content of lysine, cystine and methionine in rapeseed protein is relatively high, and the amino acid balance is better than that of soybean protein. It is a high-quality plant protein source, but the application of rapeseed meal is limited due to the presence of glucosinolates and some anti-nutritional factors such as phytic acid and polyphenol...

Claims

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Application Information

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IPC IPC(8): A23K1/00A23K1/14A23K10/12A23K20/147A23K40/00
Inventor 鞠兴荣袁建何荣王立峰顾建洪
Owner NANJING UNIV OF FINANCE & ECONOMICS
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