Method for extracting multifunctional sulforaphane from broccoli sprouting vegetable

A technology for sulforaphane and sprouts, which is applied in the directions of organic chemistry, drug combination, antitumor drugs, etc., can solve the problems of loss of sulforaphane, neglect, low extraction yield, etc., and achieves improved yield and easy biological Degradation effect

Inactive Publication Date: 2009-08-26
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Abstract
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Problems solved by technology

However, the above two methods are not aimed at extracting multifunctional sulforaphane from broccoli sprouts, and the extraction methods do not consider the influence of broccoli sprout growth cycle on sulforaphane, nor consider the broccoli sprout itself. The influence of mineral content on the extraction yield, therefore, the extraction yield is not high, and the loss of sulforaphane is large

Method used

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  • Method for extracting multifunctional sulforaphane from broccoli sprouting vegetable

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Embodiment 1

[0023] A method for extracting multifunctional sulforaphane from broccoli sprouts, comprising the following steps and process conditions:

[0024] (1) Raw material selection and pretreatment: take fresh broccoli sprouts grown for 7 days as raw material, crush through a 100-mesh sieve after freeze-drying;

[0025] (2) Two-phase hydrolysis: Weigh 100 g of the raw material treated in step (1), add 100 mL of deionized water, 100 mL of dichloromethane, 0.003 Vc, and 0.2 Na2S, and adjust the pH value to 5 with pH phosphate buffer , hydrolyzed for 8 hours at a temperature of 28°C and a magnetic stirring speed of 150 rpm; the pH phosphate buffer was a mixture of disodium hydrogen phosphate and sodium dihydrogen phosphate.

[0026] (3) Filtration, washing, purification: filter the solution obtained in step (2) with filter paper, after the filtrate is layered, get the dichloromethane layer for subsequent use, and wash the water layer with dichloromethane for 3 times to obtain a dichloro...

Embodiment 2

[0028] A method for extracting multifunctional sulforaphane from broccoli sprouts, comprising the following steps and process conditions:

[0029] (1) Raw material selection and pretreatment: take fresh broccoli sprouts grown for 6 days as raw material, crush through a 100-mesh sieve after freeze-drying;

[0030] (2) Two-phase hydrolysis: Weigh 100 g of the raw material treated in step (1), add 500 mL of deionized water, 200 mL of methylene chloride, 0.003 g of Vc, and 0.1 g of Na2S, and adjust the pH value with pH phosphate buffer 4, and extracted at 35°C for 6 hours under reflux.

[0031](3) Filtration, washing, purification: filter the solution obtained in step (2) with filter paper, after the filtrate is layered, get the dichloromethane layer for subsequent use, and the water layer is washed 4 times with dichloromethane, and the standby dichloromethane layer and the dichloromethane washing solution were combined to obtain the dichloromethane extract; after the dichloromet...

Embodiment 3

[0033] A method for extracting multifunctional sulforaphane from broccoli sprouts, comprising the following steps and process conditions:

[0034] (1) Raw material selection and pretreatment: take fresh broccoli sprouts grown for 10 days as raw material, crush through a 100-mesh sieve after freeze-drying;

[0035] (2) Two-phase hydrolysis: Weigh 100 g of the raw material treated in step (1), add 300 mL of deionized water, 150 mL of methylene chloride, 0.002 g of Vc, and 0.1 g of Na 2 S, use pH phosphate buffer solution (disodium hydrogen phosphate and sodium dihydrogen phosphate) to adjust the pH value to 6, and hydrolyze for 10 hours at a temperature of 15 ° C and a rotating speed of 300 rpm under magnetic stirring;

[0036] (3) Filtration, washing, purification: filter the solution obtained in step (2) with filter paper, after the filtrate is layered, get the dichloromethane layer for subsequent use, and the water layer is washed 2 times with dichloromethane, and the standby...

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Abstract

The invention discloses a method for extracting multifunctional sulforaphane from broccoli sprouting vegetable. The method comprises: fresh broccoli sprouting vegetable which grows for 6-10 days is taken as raw material, and is then crushed to be 100 meshes of sieve after being frozen and dried; after that, 100 parts by weight of the processed material is taken to be added with de-ionized water, methylene dichloride, 0.001-0.003 parts by weight of Vc and 0.1-0.3 parts by weight of Na2S, the pH value is adjusted to be 4-6, and the mixed solution is hydrolyzed for 6-10h at 15-35 DEG C; finally, the sulforaphane is obtained after being filtered, washed and purified. As the broccoli sprouting vegetable which grows for 6-10 days is taken as raw material, the method can effectively increase the production rate of the sulforaphane and plays the role of activation for using myrosase to hydrolyze sulpho-glucoside by adding the Vc; meanwhile, sodium sulfide is added for overcoming the interference of Fe in the material, thus improving the production rate of the sulforaphane. The obtained sulforaphane has multiple functions such as cancer resistance, oxidation resistance and the like as well as good application prospect.

Description

technical field [0001] The invention relates to a method for extracting sulforaphane, in particular to a method for extracting multifunctional sulforaphane from broccoli sprouts. Background technique [0002] Sulforaphane, also known as radionin, has the molecular formula C 6 h 11 NOS 2 , is an isothiocyanate derivative with a relative molecular weight of 177.29. It is a potent vegetable anti-cancer substance. It exists more in cruciferous vegetables, and broccoli is more abundant in cruciferous vegetables. In broccoli, the content of broccoli seeds and seedlings is higher than that of mature vegetables. Paul Talalay of Johns Hopkins Medical University in the United States published a paper proving that the content of anti-cancer component sulforaphane in broccoli sprouts is 20-30 times higher than that in mature broccoli. The results of an anti-cancer vegetable research conducted by British scientists in early 1997 showed that broccoli and Brussels sprouts are very rich...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C331/20A61P35/00
Inventor 裴世春钱丽丽左锋
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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