The invention discloses a method for detecting the content of total
glucosinolate in
broccoli sprouts. The detection method provided by the invention comprises the following steps: crushing a
bud seedling sample, adding an
ethanol solution, extracting and centrifuging to obtain an extracting solution; adding a PdCl2 solution and a
sodium carboxymethyl cellulose solution into the extracting solution, standing, and detecting by using a spectrophotometer; and substituting the detected data into a formula, and calculating to obtain the total
glucosinolate content in the
bud seedlings. According to the characteristics of broccoli
bud seedlings, high-concentration
ethanol is selected as an extraction
reagent, a
palladium chloride method is adopted for detection, the purpose of rapid and accurate detection is achieved by optimizing extraction conditions and detection conditions, and
technical support is provided for producing bud seedlings with high
glucosinolate content.