5 results about "Broccoli sprouts" patented technology

The invention relates to a method for cultivating buds and seedlings from broccoli seeds containing high-content sulforaphane, and belongs to the technical field of food. The method for cultivating bud seedlings from broccoli seeds containing high-content sulforaphane comprises the following steps: step 1, selecting seeds; step 2, live broadcast; step 3, irrigation; step 4, illumination; step 5, temperature control; 6, daily management of seedling raising; 7, disease and pest control; 8, the production environment is kept sanitary; step 9, harvesting. According to different illumination periods of plants, illumination starting time and corresponding temperature and humidity change setting are changed, the optimal environment is created according to plant growth requirements, the plants are completely in a natural growth state, and broccoli bud seedlings which are fresh and alive for 5-10 days are obtained.

The application discloses medical sulforaphane and a preparation method thereof, and belongs to the technical field of food. The preparation method of the medical sulforaphane comprises the following steps: step 1, pre-treatment of broccoli sprouts; step 2, high-temperature and low-temperature water soaking; step 3, wall breaking; step 4, filtration; step 5, cooling; step 6, filling; and step 7, ultra-fast cooling. The application further discloses medical sulforaphane prepared by the preparation method. The application can produce sulforaphane with high content and meeting the content requirement of medical research, and has high medical research value and wide market prospect.

The invention provides a method for increasing the content of glucosinolate and total flavonoids in broccoli, which comprises the following steps: performing germination treatment on broccoli seeds for 47.5-48.5 hours to obtain broccoli bud seedlings; the broccoli seedlings are transplanted into a culture medium to be subjected to seedling culture for 71.5-72.5 h, and broccoli seedlings are obtained; carrying out exogenous methyl jasmonate treatment; the treatment time of the exogenous methyl jasmonate is 0.5-48 hours; the concentration of jasmonic acid methyl ester in the exogenous jasmonic acid methyl ester treatment is 25-100 [mu] mol / L. On the premise of not influencing the growth of plants, such as dry weight, fresh weight, chlorophyll fluorescence parameters and the like, the content of glucoraphanin, brassica glucosinolate, 4-methoxy brassica glucosinolate, neoglucose brassin, total aliphatic glucosinolate, total indole glucosinolate, total glucosinolate and total flavone can be remarkably increased. And economic, efficient, convenient and accurate measures can be taken in production.

The present invention discloses a method for preparing broccoli sprout powder rich in glucoraphanin and sulforaphane, belonging to the field of food processing. The broccoli sprout powder preparation method comprises the following steps: screening broccoli seeds, washing, disinfecting, and soaking them, then placing them on a tray lined with filter paper. At an ambient temperature of 25-27°C, the seeds are first germinated with distilled water for 1-2 days, then sprayed with a sucrose solution every 8-12 hours, and allowed to germinate under natural light. The sprouts are harvested after germination for 4-7 days. The harvested sprouts are first steamed, immediately cooled to room temperature, and then soaked in a zinc sulfate solution. After removal and grinding, the pulp is placed in 2 volumes of distilled water and hydrolyzed with endogenous enzymes at 35-40°C. The hydrolyzed broccoli sprouts are freeze-dried and pulverized to obtain the broccoli sprout powder. This broccoli sprout powder is rich in glucoraphanin and sulforaphane and has high nutritional value.

The invention discloses a method for detecting the content of total glucosinolate in broccoli sprouts. The detection method provided by the invention comprises the following steps: crushing a bud seedling sample, adding an ethanol solution, extracting and centrifuging to obtain an extracting solution; adding a PdCl2 solution and a sodium carboxymethyl cellulose solution into the extracting solution, standing, and detecting by using a spectrophotometer; and substituting the detected data into a formula, and calculating to obtain the total glucosinolate content in the bud seedlings. According to the characteristics of broccoli bud seedlings, high-concentration ethanol is selected as an extraction reagent, a palladium chloride method is adopted for detection, the purpose of rapid and accurate detection is achieved by optimizing extraction conditions and detection conditions, and technical support is provided for producing bud seedlings with high glucosinolate content.