Quick testing method of rape thio-glycoside and its test board

A test board, glucosinolate technology, applied in color/spectral characteristic measurement, material analysis by observing the influence on chemical indicators, analysis by making materials undergo chemical reactions, etc., can solve the problem of separating double-low rapeseed from common rapeseed Acquisition, difficulty in achieving high-quality and high-quality double-low rapeseed, large measurement errors, etc., to achieve the effects of promoting industrialization development, fast testing speed, and low testing costs

Inactive Publication Date: 2002-03-27
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in order to develop double-low rapeseed, whether it can quickly and effectively measure the content of glucosinolate is a supporting measure that needs to be solved urgently. High Quality and Utilization of Rapeseed
As far as the determination of glucosinolates is concerned, there is currently a liquid chromatography method for the determination of glucosinolates, but this method is not only complicated, time-consuming, and expensive, but also requires expensive instruments and equipment in the laboratory, which is obviously inappropriate. It is used in planting and acquisition sites with wide distribution and large detection volume; there is also a palladium chloride method for determining glucosinolates. Although the test method is relatively simple, it is not practical due to large measurement errors and instability

Method used

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  • Quick testing method of rape thio-glycoside and its test board
  • Quick testing method of rape thio-glycoside and its test board

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Weigh 2g Huashuang No. 3 rapeseed sample and pulverize it, the fineness is 40 mesh, take 0.50g in a stoppered test tube, add 3.0ml distilled water and shake well, glucosinolate degradation reaction occurs, react at room temperature for 3-10 minutes and then filter, Take 50 μl of the filtrate and place it on the glucosinolate test plate, add 150 μl of potassium dihydrogen phosphate aqueous solution, and react for 5-15 minutes to form a colored product. According to the correlation between the glucosinolate content and the formation of the colored product, the glucosinolate content is measured by a measuring instrument to be 27.5 μmol / g.

Embodiment 2

[0014] Take 4g of Zhongshuang No. 5 rapeseed sample and pulverize it, the fineness is 50 mesh, take 0.60g into a stoppered test tube, add 3.6ml of distilled water and shake well, the glucosinolate degradation reaction occurs, react at room temperature for 8 minutes, filter, and take 60μl of filtrate Put it on the glucosinolate test plate, add 180 μl of potassium dihydrogen phosphate aqueous solution, and react for 9 minutes to form a colored product. According to the correlation between the glucosinolate content and the formation of the colored product, the glucosinolate content was determined to be 25.6 μmol / g by a measuring instrument.

Embodiment 3

[0016] It is an embodiment of the glucosinolate test plate of the present invention: take 6.2 mg of chromogenic agent (Chrom G) and dissolve it in absolute ethanol and dissolve it in 10 ml, put 10 μl in each well of the test plate, and dry it in vacuum for 1-2 hours before use , after weighing 22.8g of dipotassium hydrogen phosphate and dissolving it, fixedly dissolve 1000ml, adjust the pH to 6.0 with phosphoric acid for later use, dissolve the glucosinolate test enzyme (Gln MEN) in the above-mentioned dipotassium hydrogen phosphate buffer, and prepare a concentration of 100U / ml , put 20 μl in each well of the above test plate, freeze and vacuum dry for 2 hours.

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Abstract

The present invention relates to a quick determination method of rape-glucosinolate and its test plate, and its technical scheme includes the following steps: using 1-5g or rapeseed sample, pulverizing, using 0.20-0.80g of rapeseed powder and placing it into test tube with plug, adding 1.0-5.0 ml of distilled water, uniformly shaking to produce glucosinolate degradation reaction, after a few minuts filtering, taking 20-80 microlitres of filtrate and placing it on the test plate, adding 60-240 microlitres of potassium dihydrogen phosphate aqueous solution to make reaction, after a few minuts forming colour product, its colour is related to glucosinolate content in sample, adopting spectrophotography to measure glucosinolate content by using measurement instrument according to standard curve. Said invented method is simple and convenient, quick in test speed and low in cost.

Description

technical field [0001] The invention relates to a method for quickly measuring glucosinolate content in rapeseed and a test board used for the determination. The application of the method can effectively promote the popularization and development of double-low rapeseed, so as to improve the internal quality of rapeseed. Background technique [0002] Rapeseed is the largest oil crop in my country, rapeseed oil accounts for 60% of the total edible vegetable oil in my country, and rapeseed cake is a high-quality protein source for the feed industry. Double-low rapeseed is rapeseed with low erucic acid and low glucosinolates. It has clear limits on the two intrinsic chemical quality indicators of erucic acid and glucosinolate in rapeseed. Among them, the erucic acid content of rapeseed oil should be less than 2%. Such rapeseed The oil has high nutritional value and is beneficial to human health; the content of glucosinolate in rapeseed cake should be less than 30 μ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/27G01N21/78
Inventor 李培武张文周霞李光明杨湄吴渝汪雪芳吴宣诚仝乘风胡乐华甘东生孟子园陈小媚丁小霞陈洪谢立华
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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