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Method for extracting glucosinolates from broccoli stem and leaf wastes

A technology of glucosinolate and broccoli, which is applied in the direction of chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problem of high cost, achieve the effects of increasing added value, increasing farmland economic added value, and reducing costs

Inactive Publication Date: 2013-12-04
SHANGHAI ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Patent CN 102137678A discloses a method for extracting glucose isothiocyanate from broccoli seeds, but the raw material used in this method is mainly broccoli seeds, the cost is high, and cation exchange resin is used as the main elution method

Method used

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  • Method for extracting glucosinolates from broccoli stem and leaf wastes
  • Method for extracting glucosinolates from broccoli stem and leaf wastes

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Embodiment 1

[0025] The present invention has developed a new method for extracting glucosinolates using broccoli stem and leaf waste as the main raw material, and the implementation steps are as follows:

[0026] (1) Take the freeze-dried broccoli stem and leaf waste, crush it, pass through a 40-mesh sieve, accurately weigh the freeze-dried sample into a centrifuge tube, and keep it in a 75°C water bath for 1 minute to inactivate the endogenous myrosinase , add 2mL of 70% methanol and extract in a water bath at 75°C for 10min, then add 200μL of 0.6mol·L -1 Barium acetate to remove protein, at 8000r·min -1 , Centrifuge at 4°C for 10 min. Transfer the supernatant to a graduated tube and place on ice. The precipitate was then extracted with 2 mL of 70% methanol and centrifuged, and the extraction was repeated three times. The supernatants were combined and made up to 5 mL with 70% methanol.

[0027] (2) Weigh a certain amount of DEAE-Sephadex A-25 and soak it in 2mol L -1 In the acetic ...

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Abstract

The invention relates to physiological and biochemical technologies of vegetables, and concretely relates to a method for extracting glucosinolates from wastes comprising stems and leaves of cruciferous vegetables comprising broccoli and the like. The method is characterized in that the wastes of the stems and leaves of the vegetables which are rich in the glucosinolates and comprise broccoli, cauliflower and the like are used as raw materials, and the glucosinolates are extracted through utilizing an anion exchange column. The method has the advantages of changing of wastes into valuables, energy consumption saving, increase of the economic value added of farmlands, and agricultural income increase.

Description

technical field [0001] The invention relates to a method for extracting glucosinolates, in particular to a method for extracting glucosinolates by using an anion exchange column using broccoli stem and leaf waste as raw materials. Background technique [0002] Broccoli (Brassica oleracea var. italica), commonly known as broccoli, belongs to Brassicaceae Brassica Brassica species. It is a variety in the evolution of cabbage and cauliflower. Broccoli is tender and refreshing, with delicious flavor and rich nutrition. Regular consumption of broccoli can effectively reduce the incidence of various cancers such as lung cancer, rectal cancer, breast cancer and prostate cancer. Studies have confirmed that the anti-cancer properties of broccoli are mainly related to the glucosinolate in it. The glucosinolate in it is hydrolyzed by plant myrosinase (EC 3121311) or the radish produced by microorganisms in the human gastrointestinal tract. Sulfur (sulforapane, referred to as SF, 1-iso...

Claims

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Application Information

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IPC IPC(8): C07H15/14C07H1/08
Inventor 谢祝捷李光庆姚雪琴
Owner SHANGHAI ACAD OF AGRI SCI
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