Method for producing high-purity sulforaphene by using glucoraphanin

A technology of raffinin and rapin, which is applied in the field of raffinin production of high-purity rapin, can solve problems such as being unsuitable for industrial production, inconvenient for large-scale production, and reducing the yield of rapin, and achieves reduction of product inhibition effect and water content. , The effect of improving the purity of rapin

Inactive Publication Date: 2019-04-09
CHONGQING TECH & BUSINESS UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantages of this method are as follows: ① using radish seeds as raw materials for tissue production, the process route is long and complicated, and it is not convenient for large-scale production; A large amount of ether is used, the cost is high, and at the same time, it causes the loss of some radishin, which reduces the yield of radishin; ③In the process, a larger phase is used to extract

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for producing high-purity radhein with radish, the specific method steps are as follows:

[0028] (1) Preparation of radish reaction solution

[0029] Using commercially available radish as raw material, according to the ratio of the mass of radish (g) to the volume of purified water (mL) of 1:300, add radish to purified water, and then use 5% sulfuric acid to The pH of the solution was adjusted to 3.0, and after stirring evenly, the solution was collected for later use, which was the radish glucoside reaction solution, which was used for the next step of processing.

[0030] (2) Preparation of radishin-myrosinase-dialysis membrane tube reaction system

[0031]Using commercially available myrosinase as raw material, according to the mass ratio of myrosinase to radish in the ratio of 1:1000, slowly add myrosinase into the radish reaction liquid, and after stirring evenly, it is radish-radish Myrosinase reaction system, collected for future use. Using a commer...

Embodiment 2

[0039] A method for producing high-purity radhein with radish, the specific method steps are as follows:

[0040] (1) Preparation of radish reaction solution

[0041] Using commercially available radish as raw material, according to the ratio of the mass of radish (g) to the volume of purified water (mL) of 1:500, add radish to purified water, and then use 8% sulfuric acid to The pH of the solution was adjusted to 4.0, and after stirring evenly, the solution was collected for later use, which was the radish glucoside reaction solution, which was used for the next step of processing.

[0042] (2) Preparation of radishin-myrosinase-dialysis membrane tube reaction system

[0043] Using commercially available myrosinase as raw material, according to the mass ratio of myrosinase to radish in the ratio of 1:3000, slowly add myrosinase into the radish reaction solution, and after stirring evenly, it is radish-radish Myrosinase reaction system, collected for future use. Using a com...

Embodiment 3

[0051] A method for producing high-purity radhein with radish, the specific method steps are as follows:

[0052] (1) Preparation of radish reaction solution

[0053] Using commercially available radish as raw material, according to the ratio of the mass of radish (g) to the volume of purified water (mL) of 1:800, add radish to purified water, and then use 10% sulfuric acid to The pH of the solution was adjusted to 4.5, and after stirring evenly, the solution was collected for later use, that is, the reaction solution of radish glucoside, which was used for the next step of processing.

[0054] (2) Preparation of radishin-myrosinase-dialysis membrane tube reaction system

[0055] Using commercially available myrosinase as raw material, according to the mass ratio of myrosinase to radish in the ratio of 1:5000, slowly add myrosinase into the radish reaction liquid, and after stirring evenly, it is radish-radish Myrosinase reaction system, collected for future use. Using a co...

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Abstract

The invention belongs to the technical field of sulforaphene production, and particularly relates to a method for producing high-purity sulforaphene by using glucoraphanin. According to the method, the sulforaphene is prepared by taking glucoraphanin, myrosinase and a dialysis membrane tube as raw materials through the steps of glucoraphanin reaction solution preparation, reaction system preparation, dialysis reaction, reverse osmosis concentration, centrifugal extraction and the like. According to the method, the dialysis membrane tube is introduced for dialysis reaction, so that the in-situseparation of the product from the substrate and enzyme is realized, the degradation rate of the glucoraphanin is significantly improved, the complete retention of the myrosinase is realized, and thedialysis membrane tube can be repeatedly used for the production of the sulforaphene. The method has the characteristics of being simple in production process, stable, easy in organization amplification, high in product yield, low in equipment investment cost, low in production cost, high in comprehensive resource utilization rate and environmentally friendly. The sulforaphene prepared by the method has the characteristics of being high in purity, free of solvent residue and stable in performance, and can be widely used in the industries of medicine, food and daily chemical.

Description

technical field [0001] The invention belongs to the technical field of producing radishin, and in particular relates to a method for producing high-purity radishin by using radishin. Background technique [0002] 4-methylsulfinyl-3-butenyl glucosinolate, commonly known as radish glucoside (Glucoraphenin, C 12 h 21 NO 10 S 3 , 435.5Da), is the most abundant glucosinolate in radish seeds. Myrosinase (Myrosinase, EC 3.2.3.1), also known as β-glucosinosidase, is mainly distributed in the roots, stems, leaves, seedlings and seeds of cruciferous plants. Under the catalysis of myrosinase, the S-glycosidic bond of glucosinolate is broken to form D-glucose, hydrogen ion and unstable intermediate aglycone. After the aglycone loses the sulfate ion, it undergoes Hoffmann or Lossen rearrangement to generate Biologically active isothiocyanates or salts. Radiathin can be catalyzed by myrosinase to generate 4-isothiocyanato-1-(methylsulfinyl)-1-butene, commonly known as radishin (Sulf...

Claims

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Application Information

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IPC IPC(8): C12P13/00
CPCC12P13/002
Inventor 张杰徐萌萌刘学成张桂芝黄莉珠
Owner CHONGQING TECH & BUSINESS UNIV
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