Enteric-coated glucosinolates and beta-thioglucosidases

A technology of thioglucosidase and glucosinolate, which is applied in the field of composition of β-thioglucosidase enzyme granules, and can solve problems such as the loss of isothiocyanate

Inactive Publication Date: 2008-12-31
KRAFT FOODS GRP BRANDS LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the formation of isothiocyanates in the gastrointestinal tract may lead to the loss of isothiocyanates due to low pH favoring the formation of non-health-enhancing derivatives, especially nitrite

Method used

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  • Enteric-coated glucosinolates and beta-thioglucosidases
  • Enteric-coated glucosinolates and beta-thioglucosidases
  • Enteric-coated glucosinolates and beta-thioglucosidases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

Example 1: Enteric coated glucosinolate and myrosinase granules

A) Formation of glucosinolate and β-thioglucosidase granules

[0059]The preparation of glucosinolate and β-thioglucosidase granules can generally be performed using four sequential steps. The first of these steps is formulation and formation of a compressible mixture. After this step, the mixture is fed to a pelletizer / extruder to produce a compressed extrudate. The resulting extrudate is then fed to a spheronizer to form glucosinolate or β-thioglucosidase granules. The granules are recovered and then dried in a fluidized bed.

myrosinase granule

[0060] 35% refined lactose from Davisco Foods International, Inc. (Eden Prairie, Minnesota) and 45% microcrystalline cellulose GP-1030 from FMC BioPolymer (Philadelphia, PA) with 20% white mustard seed extract (Palmier et al., J. Aaric, Food Chern, 34:138-140 (1986)) mixing. The white mustard seed extract contains myrosinase with a specific activity of 300nmo...

Embodiment 2

[0070] Using ProCoater from Torpac (Fairfield, N, J.) with EUDRAGIT from Degussa Coating or Cellulose Acetate Phthalate ("CAP") from Eastman Chemical Co. (Kingston, T.N.) Gelatin capsules from Wonder Laboratories (White House, TN) were coated. EUDRAGIT And CAP coating can resist gastric acid. Typically, a coating is applied to provide about 8 to about 12% coating. Glucosinolates and β-thioglucosidase were stable during coating as determined by HPLC. The coated capsules were tested according to the Torpac method (Fairfield, NJ) and remained stable in 0.1N HCl at 37°C for more than 2 hours, but completely soluble in phosphate at pH 6.8 within 30 minutes at 37°C in the buffer.

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Abstract

The present invention relates to a particulate composition comprising enteric-coated glucosinolate and beta-thioglucosidase particles. The present invention further provides a method of converting glucosinolate to isothiocyanate in the small intestine comprising orally administering to a subject an enteric-coated chemoprotectant precursor composition comprising enteric-coated glucosinolate and beta-thioglucosinodase particles. In another aspect, uncoated glucosinolate and beta-thioglucosinodase particles may be provided in an enteric-coated capsule. Preferably, the glucosinolate is glucoraphanin and the beta-thioglucosidase is myrosinase. The enteric coating targets the compound for release in the small intestine where beta-thioglucosinodase enzyme converts glucosinolate to chemoprotectant isothiocyanate.

Description

technical field [0001] The present invention relates to a composition comprising enteric-coated glucosinolate and enteric-coated beta-thioglucosidase enzyme granules. The invention further provides a method of converting glucosinolates to isothiocyanates in the small intestine comprising orally administering to a subject an enteric-coated chemoprotectant precursor composition comprising an enteric-coated Coated glucosinolates and β-thioglucosidase granules. In another aspect, the uncoated glucosinolate and beta-thioglucosidase granules can be provided in enteric-coated capsules. The goal of the enteric coating is to release the chemoprotectant precursor composition in the small intestine, where β-thioglucosidase converts glucosinolates to the chemoprotectant isothiocyanate. More particularly, the chemoprotectant precursor composition comprises enteric-coated glucoraphanin and its converting enzyme myrosinase. Background technique [0002] Diet is generally believed to pla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/47A61K9/48A23L1/29A23L1/30A61P35/00A61P9/00A61P1/04A61P27/02A61K31/7028A23L29/00A23L33/00
CPCA61K9/1652A61K9/5063A61K31/375A61K31/7028A61K38/47A61P1/04A61P9/00A61P27/02A61P35/00A61P39/00A61K2300/00
Inventor L·G·维斯特N·L·温索尔A·G·高恩卡N·V·马图舍斯基N·-C·金C·J·路德维希L·L·劳伦斯
Owner KRAFT FOODS GRP BRANDS LLC
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