Application of PEI modified carbon dot-FITC compound as rate fluorescent pH probe and Cu2+ fluorescent probe

A technology of ratiometric fluorescence and fluorescent probes, applied in the intersection of biology and materials science, and in the field of chemistry, can solve the problems of cumbersome detection and achieve the effects of simple process, easy operation and control, and low production cost

Active Publication Date: 2016-11-09
QINGDAO UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, most of the above-mentioned detections only involve the detection of a single component, while practical applications often involve multi-component detection of the detection object, such as heavy metals and pH values ​​of sewage, which often require multiple detection probes or detection equipment, which makes the detection cumbersome , so exploring a multifunctional detection method has a good application prospect

Method used

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  • Application of PEI modified carbon dot-FITC compound as rate fluorescent pH probe and Cu2+ fluorescent probe
  • Application of PEI modified carbon dot-FITC compound as rate fluorescent pH probe and Cu2+ fluorescent probe
  • Application of PEI modified carbon dot-FITC compound as rate fluorescent pH probe and Cu2+ fluorescent probe

Examples

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Embodiment 1

[0041] Squeeze 30mL of fresh virgin fruit juice with a juicer and put it in a clean beaker; weigh 0.5g of β-cyclodextrin powder and add it to the virgin fruit juice, stir magnetically for 5min, transfer to an autoclave for hydrothermal reaction for 5h, and the reaction temperature is 120°C After the reaction, centrifuge (10000rpm, 15min), collect the upper layer dispersion, and filter to remove large particles; the upper layer dispersion is poured into a rotary evaporator, and rotary evaporated at a temperature of 40°C until there is no obvious Liquid, add 20mL of acetone and ethanol respectively, and centrifuge (12000rpm, 5min); repeat this centrifugation step three times, the lower sediment is CDs; prepare 1.0mg mL - 1 Add 0.1% PEI 0.2mL to 10mL CDs aqueous solution, and stir magnetically at room temperature for 0.5h; add 0.05mM FITC 10mL and continue magnetic stirring for 1h to prepare PEI-modified carbon dots-FITC complex.

[0042] The complex prepared above was divided i...

Embodiment 2

[0044] Squeeze 50mL of fresh virgin fruit juice with a juicer and put it in a clean beaker; weigh 0.7g of β-cyclodextrin powder and add it to the virgin fruit juice, stir magnetically for 7min, then transfer to an autoclave for hydrothermal reaction for 3h, and the reaction temperature is 140°C After the reaction, centrifuge (12000rpm, 10min), collect the upper layer dispersion, and filter to remove large particles; the upper layer dispersion is poured into a rotary evaporator, and rotary evaporated at a temperature of 60°C until there is no obvious Liquid, add 30mL of acetone and ethanol respectively, and centrifuge (12000rpm, 10min); repeat this centrifugation step three times, the lower sediment is CDs; prepare 0.5mgmL -1 Add 0.1% PEI 0.1mL to 10mL CDs aqueous solution, and magnetically stir for 1h at room temperature; add 0.05mM FITC 5mL and continue magnetically stirring for 2h to prepare PEI-modified carbon dots-FITC composite.

[0045] The complex prepared above was div...

Embodiment 3

[0047] Squeeze 80mL of fresh virgin fruit juice with a juicer and put it in a clean beaker; weigh 0.8g of β-cyclodextrin powder and add it to the virgin fruit juice, stir it magnetically for 10min, transfer it to the autoclave for 6h hydrothermal reaction, and the reaction temperature is 140℃ After the reaction, centrifuge (12000rpm, 10min), collect the upper layer dispersion, and filter to remove large particles; the upper layer dispersion is poured into a rotary evaporator, and rotary evaporated at a temperature of 60°C until there is no obvious Liquid, add 20mL of acetone and ethanol respectively, and centrifuge (12000rpm, 10min); repeat this centrifugation step three times, the lower sediment is CDs; prepare 0.5mgmL -1 Add 0.1% PEI 0.2mL to 10mL of CDs aqueous solution, and stir magnetically at room temperature for 1h; add 0.05mM FITC 5mL and continue magnetic stirring for 2h to prepare PEI-modified carbon dots-FITC complex.

[0048] The complex prepared above was divided ...

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Abstract

The invention discloses application of a PEI modified carbon dot-FITC compound as a rate fluorescent pH probe and a Cu2+ fluorescent probe. Cherry tomatoes are used as raw materials, carbon dots with fluorescence insensitive to pH changes are prepared through hydro-thermal synthesis, then CDs are modified with PEI, amidogen on PEI react with isothiocyanic acid radicals on FITC molecules, and the compound is formed. The responsivity of fluorescence of FITC in the compound to the pH changes is high, and a rate probe based on CDs and FITC fluorescence is used for pH detection; amidogen on PEI and Cu2+ are subjected to coordination, so that the fluorescence intensity of CDs and FITC is reduced along with increase of the concentration of Cu2+ in a solution, and Cu2+ detection is achieved. According to the constructed fluorescent probe, selected materials are novel, the advantages of being high in sensitivity, good in selectivity, easy to operate, wide in linear range, low in detecting limit and the like are achieved, and the constructed fluorescent probe can be used for efficient detection of the pH and Cu2+ in the aqueous solution and a biological sample.

Description

technical field [0001] The invention belongs to the interdisciplinary field of chemistry, biology and material science, relates to material preparation, biological and environmental ion detection and pH detection, specifically relates to the preparation of PEI modified carbon dot-FITC complex and its use as a ratiometric fluorescent pH probe and Cu 2+ Application of fluorescent probes. Background technique [0002] Quantum dots usually refer to semiconductor nanocrystals whose size is smaller than or close to its excitonic Bohr radius, and are a class of nanoparticles with unique optical and electrical properties. Compared with one-dimensional or two-dimensional carbon nanomaterials, carbon dots (CDs) prepared from non-toxic biological raw materials have higher specific surface area and more edge active sites, which makes CDs more effective in biotechnology than large-scale carbon materials. The field of sensing and catalysis has more extensive and efficient application pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06G01N21/64
CPCC09K11/06C09K2211/1466G01N21/6428
Inventor 桂日军金辉朱欣欣徐显朕王宗花
Owner QINGDAO UNIV
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