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Preparation method of magnetic particle with surface modified with isothiocyanato active group

A technology of surface modification and isothiocyanate, which is applied in the preparation method of peptides, biochemical equipment and methods, magnetic materials, etc., can solve the problems of no synthesis, etc., and achieve the effect of easy coupling and immobilization, and short activation time

Active Publication Date: 2010-12-01
XIAN GOLDMAG NANOBIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] As a homogeneous bifunctional cross-linking agent, PDC was first used to activate solid-phase substrates for micro-sequencing reactions of proteins [Machleidt, W.; Wachter, E.; Scheulen, M.; Otto J.Febs Lett.1973 , 37, 217-220. Walker, JE.; Fearnley, IM.; Blows, RA. Biochem. J. 1986, 237: 73-84. Aebersold, RH.; Pipes, G.; Hood, LE.; SBH.Electrophoresis.1988,9,520-530], but there is no report about synthesizing functionalized magnetic particles with colloidal nano or micron particles containing amino or sulfhydryl functional groups

Method used

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  • Preparation method of magnetic particle with surface modified with isothiocyanato active group
  • Preparation method of magnetic particle with surface modified with isothiocyanato active group
  • Preparation method of magnetic particle with surface modified with isothiocyanato active group

Examples

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preparation example Construction

[0042] (3) Preparation of functionalized magnetic composite particles: magnetically separate the nano- and micron-scale solid-phase magnetic particles placed in the liquid phase with amino or sulfhydryl functional groups on the surface, discard the supernatant; add the PDC solution prepared above , shaking and reacting at room temperature for 10 hours, magnetic separation, discarding the supernatant, and washing repeatedly with an organic solvent to obtain functionalized magnetic composite particles with isothiocyanate (-N=C=S) active groups on the surface.

[0043] The organic solvent used in the above-mentioned preparation method: 3-aminopropyltrimethoxysilane, 3-aminopropyltriethoxysilane, 3-amino or mercaptopropane, 3-amino or mercaptohexane, methanol, acetone, Ethanol, pyridine, N,N-dimethylformamide (DMF) and dimethylsulfoxide.

Embodiment 1

[0046] This example aims to illustrate the practicability of preparing the functionalized magnetic composite particles of the present invention.

[0047] 1. Preparation of PDC solution

[0048] Weigh 0.1 g of phenylenediisothiocyanate solid powder, and dissolve it in 50 mL of a mixed solution of DMF and pyridine (DMF:pyridine=9:1).

[0049] 2. Preparation of functionalized magnetic composite particles

[0050] The nano- and micron-sized solid-phase magnetic particles (the body of the solid-phase particles are Fe oxide powder) dispersed in the absolute ethanol phase with amino or mercapto functional groups on the surface are subjected to magnetic separation, and the supernatant is discarded. Take 105-200 mg of solid-phase magnetic particles modified with surface amino groups or sulfhydryl groups (the content of amino groups or sulfhydryl groups on the surface of the magnetic particles is 100-300 μmoL g -1 ), add 15-25mL of the above-prepared PDC solution therein, shake and re...

Embodiment 2

[0052] This example aims to illustrate the practicability of preparing the functionalized magnetic composite particles of the present invention.

[0053] 1. Preparation of PDC solution

[0054] Weigh 0.1 g of phenylenediisothiocyanate solid powder, and dissolve it in 50 mL of a mixed solution of DMF and pyridine (DMF:pyridine=9:1).

[0055] 2. Preparation of functionalized magnetic composite particles

[0056] The nano- and micron-sized solid-phase magnetic particles (the body of the solid-phase particles are Fe oxide powder) dispersed in the absolute ethanol phase with amino or mercapto functional groups on the surface are subjected to magnetic separation, and the supernatant is discarded. Take 300 mg of surface amino or sulfhydryl modified solid-phase magnetic particles (the content of amino or sulfhydryl on the surface of the magnetic particle is 300 μmoL g -1 ), add 50mL of the above-prepared PDC solution therein, and shake and react in a constant temperature shaker at 1...

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Abstract

The invention provides a preparation method of a magnetic particle with surface modified with an isothiocyanato active group and a special application mode thereof. The preparation method comprises the following steps of: dispersing processed nano and micron grade solid-phase particles with surface modified with amino or sulfydryl active groups into an organic solvent; and mixing the particles with phenylene diisothio cyanate (PDC) for reaction to obtain a functional magnetic complex particle with surface modified with an isothiocyanato (-N=C=S) active group as a functional group. The prepared magnetic particle with surface modified with the isothiocyanato active group realizes that the common functional magnetic particle is rapidly coupled into a biomolecule and avoids toxic and side effects by adopting glutaric dialdehyde as a cross-linking agent in routine operation.

Description

technical field [0001] The present invention relates to the practical application and preparation method of functionalized magnetic composite particles, in particular to the preparation method of magnetic particles modified with isothiocyanate active groups on the surface and the application of functionalized magnetic composite particles derived from the preparation method . Background technique [0002] Functionalized magnetic particles with amino or sulfhydryl groups on the surface have been widely used in the field of biomedicine. Glutaraldehyde is usually used as a coupling agent when coupling biomolecules to these particles [Liu, X.; Xing, J.; Guan, Y.; Shan, G.; Liu, H. Colloids Surf., A. 2004, 238, 127-131. Li, Y.; Xu, X.; Deng, C.; Yang, P. ; Zhang, X.J Proteome Res.2007, 6(9):3849-3855. Li, GY.; Huang, KL.; Jiang, YR.; , 405-412.49-54.], but using glutaraldehyde as a cross-linking agent not only has greater toxicity, but also requires a longer reaction time (2-10h...

Claims

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Application Information

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IPC IPC(8): H01F1/00B22F1/00C12Q1/68C07K16/00C07K1/22C12N15/10C12N11/00C12N5/00
Inventor 崔亚丽唐一通陈超耿婷婷
Owner XIAN GOLDMAG NANOBIOTECH
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