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Gardenia polysaccharide and preparation method and application thereof

A kind of gardenia polysaccharide, gardenia technology, applied in pharmaceutical formulations, medical preparations containing active ingredients, digestive system and other directions, can solve the problem of limited research and development, no liver protection ingredients, can not fully represent the gardenia jaundice and liver protection, etc. question

Active Publication Date: 2020-05-12
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have determined that geniposide is the main active substance, but geniposide has hepatotoxicity, which limits its further research and development, and also contradicts the widespread use of geniposide in clinical practice, suggesting that geniposide cannot fully represent geniposide. Anti-yellowing and liver-protecting effect substances, gardenia may also contain other anti-yellowing and liver-protecting ingredients
However, there are no relevant reports on other hepatoprotective ingredients in the prior art

Method used

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  • Gardenia polysaccharide and preparation method and application thereof
  • Gardenia polysaccharide and preparation method and application thereof
  • Gardenia polysaccharide and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The preparation method of gardenia polysaccharide, the step comprises:

[0037] Gardenia medicinal materials were sun-dried, crushed and passed through an 80-mesh sieve to obtain Gardenia powder; the Gardenia powder was soaked in ethanol for 24 hours to degrease, filtered, and the dregs were dried in the air, wherein the solid-to-liquid ratio of Gardenia powder and ethanol was 1g:8mL; Extract the dregs with 100°C hot water 20 times the mass of the dregs for 3 times, 3 hours each time, filter, combine the filtrate, concentrate the filtrate to 1 / 4 of the original filtrate, and obtain the concentrated solution A, which is 3500×g Centrifuge for 10 minutes, take the supernatant to obtain the water extract; add ethanol to the water extract to make the alcohol concentration reach 70%, let it stand overnight at room temperature, filter and collect the precipitate, further soak the precipitate twice with 95% absolute ethanol, and then place it at 40°C Oven for 20 minutes, then a...

Embodiment 2

[0040]Healthy male C57BL / 6J mice, weighing 20±2g, were provided by Shanghai Lingchang Animal Co., Ltd. After one week of adaptive feeding, the mice were randomly divided into normal control group, model control group, ursodeoxycholic acid positive control group, and gardenia polysaccharide group, with 10 mice in each group. The positive control group was given 115 mg / kg of ursodeoxycholic acid by intragastric administration, the normal control group and the model control group were given intragastric administration of equal volume of normal saline, and the gardenia polysaccharide group was given 400 mg / kg of gardenia polysaccharide (dissolved in normal saline) by intragastric administration. Once a day, continuous administration for 14 days. After 4 hours of administration on the 12th day of the test, except the normal control group, the other groups were intragastrically administered α-naphthalene isothiocyanate (ANIT) 50 mg / kg to replicate the acute cholestatic liver injury ...

Embodiment 3

[0052] Healthy male C57BL / 6J mice, weighing 22±2g, were provided by Beijing Weitong Lihua Experimental Animal Co., Ltd. After one week of adaptive feeding, the mice were randomly divided into normal control group, model control group and gardenia polysaccharide group. Except for the normal control group given normal feed, other groups were given 0.025% 3,5-diethoxycarbonyl-1,4-dihydro-2,4,6-collidine (3,5-diethoxycarbonyl- 1,4-dihydroxychollidine, DDC) toxic feed, to reproduce the chronic cholestasis liver injury model, 1 week after the model induction, the gardenia polysaccharide group was given 800mg / kg gardenia polysaccharide (dissolved in normal saline), and the normal group and the model The rats in the group were given the same amount of normal saline by intragastric administration, once a day. During the administration period, the general symptoms of the animals during the experiment were closely observed. After 4 weeks of drug intervention (5 weeks after DDC modeling)...

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Abstract

The invention discloses gardenia polysaccharide and an extraction method thereof. The gardenia polysaccharide is prepared from the following raw materials (by mass): 41 to 56 percent of total sugar, 34 to 45 percent of uronic acid and 7 to 10 percent of protein. The preparation method of the gardenia polysaccharide comprises the following steps: crushing a gardenia medicinal material, degreasing,adding water, extracting, precipitating with ethanol, repeatedly freeze-thawing to remove protein, dialyzing, and freeze-drying. The gardenia polysaccharide prepared by the method can improve cholestatic liver injury induced by alpha-naphthalene isothiocyanate (ANIT) and 3, 5-diethoxycarbonyl-1, 4-dihydro-2, 4, 6-trimethylpyridine (DDC), and reduce fibrosis induced by carbon tetrachloride (CCL4).And the liver protection effect of the gardenia polysaccharide is not reported in literatures, which indicates that the gardenia polysaccharide has obvious application prospects in the preparation ofliver protection drugs, especially in the preparation of anti-cholestatic liver injury and anti-hepatic fibrosis drugs.

Description

technical field [0001] The invention relates to a gardenia polysaccharide and its preparation method and application, belonging to the technical field of traditional Chinese medicine. Background technique [0002] A variety of chronic liver diseases such as liver cirrhosis of various causes, primary sclerosing cholangitis, primary biliary cirrhosis, alcoholic hepatitis, drug-induced liver disease, viral hepatitis, autoimmune hepatitis, liver tumors, etc., often Accompanied by intrahepatic cholestasis (IHC), that is, the formation, secretion and excretion of bile flow are impaired, and the bile flow cannot flow into the duodenum normally, but stays in the liver and enters the blood circulation. These liver diseases are also called Cholestatic liver disease / liver injury. As the disease progresses, intrahepatic cholestasis can aggravate liver damage and liver fibrosis / cirrhosis, which can lead to liver failure or even death in severe cases. For intrahepatic cholestatic liver ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P11/00A61P1/16
CPCC08B37/006C08B37/0003A61K31/715A61P11/00A61P1/16
Inventor 马越鸣吴家胜王天明方溯
Owner SHANGHAI UNIV OF T C M
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