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Preservative composition of blood DNA

A technology of preservative and composition, which is applied in the field of DNA preservation, and can solve problems affecting test results, etc.

Inactive Publication Date: 2017-06-30
上海迅伯生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The amount of blood DNA is a very important indicator during the detection process. If the white blood cells or nuclear red blood cells rupture, the DNA in the genome will degrade and enter the plasma to contaminate free DNA, which will seriously affect the test results.

Method used

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  • Preservative composition of blood DNA
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  • Preservative composition of blood DNA

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Calculated in parts by weight, the blood DNA preservative composition includes:

[0077]

[0078]

[0079] Wherein, the preservative is diazolidinyl urea, diazolidinyl urea, imidazolidinyl urea, and the weight ratio of the diazolidinyl urea to the diazolidinyl urea and the imidazolidinyl urea is 1:2.1:4.1; the anticoagulant is tripotassium edetate and disodium edetate, the weight ratio of tripotassium edetate to disodium edetate 2.2:1; the nuclease inhibitor is a vanadyl ribonucleoside complex and a 4-formylbenzoic acid Schiff base vanadium complex, and the vanadyl ribonucleoside complex and the 4-formylbenzene The weight ratio of formic acid Schiff base vanadium complex is 0.24:1; the metabolic inhibitor is gluconic acid; the cell membrane protecting agent is glycine; and the water is sterilized ultrapure water.

[0080] The preparation steps of the 4-formylbenzoic acid Schiff base vanadium complex are as follows:

[0081] (1) Add 4-formylbenzoic acid, sodium h...

Embodiment 2

[0086] Calculated in parts by weight, the blood DNA preservative composition includes:

[0087]

[0088] Wherein, the preservative is diazolidinyl urea; the anticoagulant is tripotassium edetate and disodium edetate, and the tripotassium edetate and the ethylenediamine The weight ratio of disodium tetraacetate is 2.2:1; The nuclease inhibitor is vanadyl ribonucleoside complex and 4-formylbenzoic acid Schiff base vanadium complex, and the vanadyl ribonucleoside complex and The weight ratio of the 4-formylbenzoic acid Schiff base vanadium complex is 0.24:1; the metabolic inhibitor is gluconic acid; the cell membrane protective agent is glycine, and the water is ultra-pure water after sterilization water.

[0089] The preparation steps of the 4-formylbenzoic acid Schiff base vanadium complex are as follows:

[0090] (1) Add 4-formylbenzoic acid, sodium hydroxymethylglycinate, 1,3-bis(3-hydroxypropyl)-1,1,3,3-tetramethyldisiloxane, Dicyclohexylcarbodiimide, 4-dimethylaminopy...

Embodiment 3

[0095] Calculated in parts by weight, the blood DNA preservative composition includes:

[0096]

[0097] Wherein, the preservative is diazolidinyl urea; the anticoagulant is tripotassium edetate and disodium edetate, and the tripotassium edetate and the ethylenediamine The weight ratio of disodium tetraacetate is 2.2:1; The nuclease inhibitor is vanadyl ribonucleoside complex and 4-formylbenzoic acid Schiff base vanadium complex, and the vanadyl ribonucleoside complex and The weight ratio of the 4-formylbenzoic acid Schiff base vanadium complex is 0.24:1; the metabolic inhibitor is gluconic acid; the cell membrane protective agent is glycine, and the water is ultra-pure water after sterilization water.

[0098] The preparation steps of the 4-formylbenzoic acid Schiff base vanadium complex are as follows:

[0099] (1) Add 4-formylbenzoic acid, sodium hydroxymethylglycinate, 1,3-bis(3-hydroxypropyl)-1,1,3,3-tetramethyldisiloxane, Dicyclohexylcarbodiimide, 4-dimethylaminopy...

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Abstract

The invention provides a preservative composition of blood DNA. The preservative composition of blood DNA comprises, by weight, 20 to 50 parts of a preservative, 5 to 15 parts of an anticoagulant, 0.5 to 5 parts of a nuclease inhibitor, 1 to 5 parts of a metabolic inhibitor and 40 to 70 parts of water, wherein the nuclease inhibitor comprises one or more of diatomite, bentonite, ethyl alcohol, formamide, vanadyl-ribonucleoside complex, dithioerythritol, diethyl pyrocarbonate, proteinase-K, heparin, beta-mercaptoethanol, hydroxylamine-O-copper ions, ammonium sulfate, dithiothreitol, cysteine, and 4-formylbenzoic acid and oxovanadium Schiff base complex.

Description

technical field [0001] The invention relates to the field of DNA preservation, in particular, the invention relates to a blood DNA preservation agent composition. Background technique [0002] Plasma preservation tube is a kind of blood collection tube that directly draws whole blood, can stably preserve blood DNA (cfDNA) and circulating tumor DNA (ctDNA) at room temperature, and is stable for storage and transportation. Although there is only a small amount of cell-free DNA in the plasma of healthy individuals, increased or decreased levels of cell-free DNA in blood circulation are associated with many clinical diseases. The non-invasive prenatal screening of pregnant women, which is accepted and recognized for its safety and accuracy, is to diagnose fetal genetic diseases through the detection and analysis of fetal blood DNA in maternal blood. The detection and analysis of circulating tumor DNA in human blood has created conditions for early screening of tumors. At the s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/00
CPCA01N1/00
Inventor 陈金丝陈睿博唐慧菁陆熙平
Owner 上海迅伯生物科技有限公司
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