The invention discloses a
peripheral blood TCR marker of acute
myeloid leukemia and a detection kit and application of the
peripheral blood TCR marker. The marker comprises at least one of proteins of which the sequences are as shown in SEQ ID NO.1-100. On the basis of a high-
throughput sequencing method, only a small amount of
peripheral blood needs to be adopted,
RNA is extracted, an immune map
library is established by treating a sample, then through high-
throughput sequencing and TCR
data analysis, firstly, a characteristic TCR sequence in the
peripheral blood of acute
myeloid leukemia is determined, then a test result of a to-be-tested sample is compared with the characteristic TCR sequence. Therefore, whether the patient suffers from acute
myeloid leukemia is determined. According to the kit, a huge number of acute myeloid
leukemia specific TCR sequences can be compared at the same time, and compared with single detection of one or more markers, the kit has higher specificity and accuracy, and the diagnosis efficiency is improved.