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Application of embellin in preparation of medicament for inhibiting angiogenesis

An angiogenesis and signaling quinone technology, applied in the field of application of signaling quinones in the preparation of anti-angiogenesis drugs, can solve the problems of lack of signaling quinones, etc.

Inactive Publication Date: 2008-10-08
EAST CHINA NORMAL UNIVERSITY
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  • Claims
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AI Technical Summary

Problems solved by technology

[0005] Embelin is a benzoquinone compound purified from the vine fruit of alba quinquefolium. Although it has been reported in the literature that embelin can directly act on tumor cells to have anti-tumor effects, as well as antibacterial and anti-inflammatory effects. , anti-fertility, etc., but there is no report that sinoquinone can inhibit angiogenesis, or inhibit tumors by inhibiting angiogenesis, and there is no report that sinoquinone can be used as an angiogenesis-inhibiting drug

Method used

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  • Application of embellin in preparation of medicament for inhibiting angiogenesis
  • Application of embellin in preparation of medicament for inhibiting angiogenesis
  • Application of embellin in preparation of medicament for inhibiting angiogenesis

Examples

Experimental program
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Effect test

experiment example 1

[0027] Experimental Example 1: Signal quinone inhibits the proliferation of human microvascular endothelial cells (HMEC-1) cells

[0028] Purpose and principle: MTS assay was used in the cell proliferation experiment. The MTS assay is an assay that uses a colorimetric method to determine the number of cell proliferation in living cells. NADPH dehydrogenase converts MTS tetrazolium salt compound into a colored substance soluble in tissue culture fluid, and its color depth is highly correlated with the number of living cells of the cell line within a certain range. This model can be used to evaluate the effect of drugs on cell proliferation.

[0029] Method: Inoculate the HMEC-1 cell suspension in a 96-well plate (5000 cells per well), add medium containing different concentrations of signaloquinone for 48-72 hours, then add 20 μl of chromogen to each well, and incubate After 1-4 hours, read the light absorbance value in each well at 490 nm with a microplate reader.

[0030] ...

Embodiment 2

[0031] Implementation example 2: Signal quinone inhibits the scratch migration experiment of HMEC-1 cells in vitro

[0032] Purpose and principle: Cell migration test is also called in vitro scratch test. In this experiment, scratches were made on the overgrown monolayer endothelial cells in the culture plate, observed under a microscope, and the migration of cells at the edge of the scratches to the scratched site was recorded to evaluate the effect of drugs on the migration ability of endothelial cells.

[0033] Method: Divide 8×10 4 Cells per ml were seeded in a 6-well plate, and when the cells grew to over 90%, draw a line in each well with a 1 ml tip of a micropipette. Wash the cell debris with PBS, then add VEGF-containing 165 and medium containing different drug concentrations in 5% CO 2 Incubate in an incubator at 37°C for about 24 hours until VEGF 165 The group is full.

[0034] Results and evaluation: Observing and taking pictures under a microscope, the results...

Embodiment 3

[0035] Implementation Example 3: Signal quinone inhibits migration of HMEC-1 cell modified Boyden chamber

[0036] Purpose and principle: The Boyden chamber migration experiment modified by endothelial cells adopts Millicell (Millopore) chamber, which is a type of petri dish that can be inserted into a cell culture plate. The bottom layer has a polycarbonate filter membrane with a pore size of 8 μm. Under the induction, cells can permeate one side of the membrane and adhere to the other side containing high concentrations of growth factors. This experiment can detect the effect of drugs on the ability of endothelial cells to resist migration.

[0037] Method: Add 1% gelatin to the 24-well plate and incubate at 37°C for 30 minutes, discard the gelatin, and wash three times with PBS. Add 600 μl containing VEGF to the 24-well plate outside the chamber 165 endothelial cell culture medium. Insert 20,000 cells into the small chamber, then add 50 μL of different concentrations of s...

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Abstract

The invention relates to a novel use of Chinese herbal medicine monomer embelin, in particular to an application of the embelin in a preparation of a drug for inhibiting angiogenesis. The embelin is applied in the inhibition of angiogenesis dependent disease such as mammary cancer, colon cancer, lymphoma, acute myelocytic leukemia, rheumatoidarthritis, psoriasis, diabetic syndrome, hemangioma, etc. Experiments show that the embelin has remarkable inhibitory action in proliferation experiment, migration experiment, invasion experiment, cell tabulation experiment, the angiogenesis of chick embryo allantois, etc. in the human microvascular endothelial cells (HMEC-1), thereby being used for preparing the drug for inhibiting the angiogenesis.

Description

technical field [0001] The invention relates to a new application of a Chinese herbal medicine monomer Embelin, in particular to the application of Embelin in the preparation of drugs for inhibiting angiogenesis. Background technique [0002] Angiogenesis is the process of generating new blood vessels on the basis of existing blood vessels, including five basic processes: ① degradation of vascular basement membrane; ② migration of endothelial cells through the basement membrane to the perivascular matrix; ③ endothelial cell proliferation, mutual adhesion and connection; ④Formation of a lumen-like structure; ⑤Matrix remodeling and smooth muscle cell envelopment and blood vessel anastomosis to form a vascular network. [0003] Angiogenesis occurs under both physiological and pathological conditions. Physiological angiogenesis mainly occurs in embryonic development and female physiological cycle, which is critical to normal development; pathological angiogenesis is an important...

Claims

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Application Information

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IPC IPC(8): A61K31/122A61P17/06A61P19/02A61P25/02A61P27/02A61P29/00A61P35/00A61P35/02
Inventor 易正芳李双喜张京赖力张晓丽卢彬彬易庭芳刘明耀
Owner EAST CHINA NORMAL UNIVERSITY
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